Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,911,962	G→C	6.7%	R103P (CGC→CCC)	PP_3452 →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,911,962	0	G	C	6.7%	219.7 / 2.5	69	R103P (CGC→CCC)	PP_3452	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (26/38);  new base C (3/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAT  >  NC_002947/3911814‑3912107                                                                                                                                                     |                                                                                                                                                  ttCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATgcg                                                                                                                                                   >  4:197740/1‑149 (MQ=255) ttCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATgcg                                                                                                                                                   >  8:228483/1‑149 (MQ=255) ttCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATgcg                                                                                                                                                   >  8:232841/1‑149 (MQ=255)  tCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCAGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGAGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATgcgc                                                                                                                                                  >  2:310350/1‑149 (MQ=255)     tCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTg                                                                                                                                               >  7:276142/1‑149 (MQ=255)        gCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGt                                                                                                                                             >  3:77229/1‑148 (MQ=255)           aCCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         >  6:140204/1‑149 (MQ=255)             cAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGa                                                                                                                                       <  4:302070/149‑1 (MQ=255)              aaGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  4:31859/149‑1 (MQ=255)               aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  1:310350/149‑1 (MQ=255)               aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  1:243552/149‑1 (MQ=255)               aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  5:301151/149‑1 (MQ=255)                   ttCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCAt                                                                                                                                 >  3:246851/1‑149 (MQ=255)                     cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  7:228483/148‑1 (MQ=255)                     cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  3:197740/148‑1 (MQ=255)                     cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  7:232841/148‑1 (MQ=255)                       ccTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  6:34322/140‑1 (MQ=255)                       ccTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      >  5:34322/1‑140 (MQ=255)                              gTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACACTGGGCATCCCCCTGGTGATCGAAGGCATCCCGATAGcac                                                                                                                      >  8:266997/1‑147 (MQ=255)                               tccttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         >  1:170320/1‑129 (MQ=255)                               tccttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         <  2:170320/129‑1 (MQ=255)                                  ttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAgg                                                                                                                                    >  8:50639/1‑131 (MQ=255)                                  ttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAgg                                                                                                                                    <  7:50639/131‑1 (MQ=255)                                       gTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCgg                                                                                                             >  6:166770/1‑149 (MQ=255)                                        ttCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  3:301589/123‑1 (MQ=255)                                        ttCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      >  4:301589/1‑123 (MQ=255)                                           aTCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCTTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGtgtg                                                                                                         >  5:361277/1‑149 (MQ=255)                                           aTCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGtgtg                                                                                                         >  8:89776/1‑149 (MQ=255)                                              gCCCTGCTCGGGAGGCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACGCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGTTAGCCCTGCGCCCGGTGtggtt                                                                                                      <  7:266997/149‑1 (MQ=255)                                               cccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttg                                                                                                     <  4:77229/149‑1 (MQ=255)                                                ccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttgg                                                                                                    >  4:68033/1‑149 (MQ=255)                                                ccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttgg                                                                                                    >  7:209284/1‑149 (MQ=255)                                                       ggCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGttgttg                                                                                                        <  3:168216/138‑3 (MQ=255)                                                       ggCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGATGtgg                                                                                                        >  4:168216/1‑138 (MQ=255)                                                        gCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCGTCACCCTGGGCATGCGCCGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGGTGGTGCTCggg                                                                                            <  5:155748/149‑1 (MQ=255)                                                        gCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            <  7:89776/149‑1 (MQ=255)                                                          aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  1:215854/149‑1 (MQ=255)                                                          aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  3:259832/149‑1 (MQ=255)                                                          aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  2:9027/149‑1 (MQ=255)                                                                   gTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGt                                                                                 <  4:246851/149‑1 (MQ=255)                                                                      gTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCt                                                                              <  6:308166/149‑1 (MQ=255)                                                                         ctgcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGt                                                                            <  8:276142/148‑1 (MQ=255)                                                                         ctgcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTAATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCAACCGTGCTGTa                                                                           >  1:275015/1‑149 (MQ=255)                                                                           gcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATg                                                                         >  1:75789/1‑149 (MQ=255)                                                                             aTTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGcc                                                                       <  5:131344/149‑1 (MQ=255)                                                                                     cgccgcAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGt                                                               <  2:75789/149‑1 (MQ=255)                                                                                       ccgcAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGc                                                             <  5:140204/149‑1 (MQ=255)                                                                                              cATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            <  5:45439/111‑1 (MQ=255)                                                                                              cATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            >  6:45439/1‑111 (MQ=255)                                                                                               aTCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTg                                                     >  2:259270/1‑149 (MQ=255)                                                                                                    ttCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          >  2:316917/1‑139 (MQ=255)                                                                                                    ttCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          <  1:316917/139‑1 (MQ=255)                                                                                                       gACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTACTACCGGTGCGGCGCTg                                             <  6:361277/149‑1 (MQ=255)                                                                                                             cTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCAt                                        <  8:209284/148‑1 (MQ=255)                                                                                                                  ccTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATtcttct                                  <  1:259270/149‑1 (MQ=255)                                                                                                                    tgctgTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                >  2:278200/1‑149 (MQ=255)                                                                                                                         ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                >  4:223097/1‑144 (MQ=255)                                                                                                                         ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                <  3:223097/144‑1 (MQ=255)                                                                                                                         ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCa                            <  5:166770/148‑1 (MQ=255)                                                                                                                            aTTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          >  7:306861/1‑115 (MQ=255)                                                                                                                            aTTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          <  8:306861/115‑1 (MQ=255)                                                                                                                                 ttcgatcaCCAGGCGCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatca                   >  7:322870/5‑149 (MQ=255)                                                                                                                                 ttcgatcaCCAGGCGCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatca                   >  3:786/5‑149 (MQ=255)                                                                                                                                      tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatc                    >  1:41600/1‑143 (MQ=255)                                                                                                                                      tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatc                    <  2:41600/143‑1 (MQ=255)                                                                                                                                      tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTgg              <  2:275015/149‑1 (MQ=255)                                                                                                                                        aCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCAcccaggt            >  6:324543/1‑144 (MQ=255)                                                                                                                                         cccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGt            >  7:30779/1‑148 (MQ=255)                                                                                                                                          ccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATg                          >  2:268807/1‑133 (MQ=255)                                                                                                                                          ccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATg                          <  1:268807/133‑1 (MQ=255)                                                                                                                                          caggcgCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGa          <  4:786/144‑1 (MQ=255)                                                                                                                                             gcgCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGc       <  8:322870/147‑1 (MQ=255)                                                                                                                                                  tGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAt  <  8:30779/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTCCTCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAT  >  NC_002947/3911814‑3912107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑