Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,911,965 T→G 7.0% L104R (CTG→CGG) ‡ PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,911,9650TG 7.0% 212.7 / 2.8 69L104R (CTG→CGG) ‡PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (27/37);  new base G (2/3);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAT  >  NC_002947/3911818‑3912107
                                                                                                                                                   |                                                                                                                                              
tCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTg                                                                                                                                               >  7:276142/1‑149 (MQ=255)
   gCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGt                                                                                                                                             >  3:77229/1‑148 (MQ=255)
      aCCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         >  6:140204/1‑149 (MQ=255)
        cAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGa                                                                                                                                       <  4:302070/149‑1 (MQ=255)
         aaGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  4:31859/149‑1 (MQ=255)
          aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  1:310350/149‑1 (MQ=255)
          aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  1:243552/149‑1 (MQ=255)
          aGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAg                                                                                                                                     <  5:301151/149‑1 (MQ=255)
              ttCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCAt                                                                                                                                 >  3:246851/1‑149 (MQ=255)
                cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  7:232841/148‑1 (MQ=255)
                cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  7:228483/148‑1 (MQ=255)
                cACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATc                                                                                                                                <  3:197740/148‑1 (MQ=255)
                  ccTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  6:34322/140‑1 (MQ=255)
                  ccTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      >  5:34322/1‑140 (MQ=255)
                         gTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACACTGGGCATCCCCCTGGTGATCGAAGGCATCCCGATAGcac                                                                                                                      >  8:266997/1‑147 (MQ=255)
                          tccttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         <  2:170320/129‑1 (MQ=255)
                          tccttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATc                                                                                                                                         >  1:170320/1‑129 (MQ=255)
                             ttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAgg                                                                                                                                    <  7:50639/131‑1 (MQ=255)
                             ttcctGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAgg                                                                                                                                    >  8:50639/1‑131 (MQ=255)
                                  gTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCgg                                                                                                             >  6:166770/1‑149 (MQ=255)
                                   ttCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      >  4:301589/1‑123 (MQ=255)
                                   ttCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGaa                                                                                                                                      <  3:301589/123‑1 (MQ=255)
                                      aTCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCTTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGtgtg                                                                                                         >  5:361277/1‑149 (MQ=255)
                                      aTCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGtgtg                                                                                                         >  8:89776/1‑149 (MQ=255)
                                         gCCCTGCTCGGGAGGCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACGCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGTTAGCCCTGCGCCCGGTGtggtt                                                                                                      <  7:266997/149‑1 (MQ=255)
                                          cccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttg                                                                                                     <  4:77229/149‑1 (MQ=255)
                                           ccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttgg                                                                                                    >  7:209284/1‑149 (MQ=255)
                                           ccTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGtggttgg                                                                                                    >  4:68033/1‑149 (MQ=255)
                                                  ggCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGttgttg                                                                                                        <  3:168216/138‑3 (MQ=255)
                                                  ggCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGATGtgg                                                                                                        >  4:168216/1‑138 (MQ=255)
                                                   gCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCGTCACCCTGGGCATGCGCCGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGGTGGTGCTCggg                                                                                            <  5:155748/149‑1 (MQ=255)
                                                   gCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            <  7:89776/149‑1 (MQ=255)
                                                     aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  1:215854/149‑1 (MQ=255)
                                                     aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  2:9027/149‑1 (MQ=255)
                                                     aGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGgt                                                                                          <  3:259832/149‑1 (MQ=255)
                                                              gTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGt                                                                                 <  4:246851/149‑1 (MQ=255)
                                                                 gTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCt                                                                              <  6:308166/149‑1 (MQ=255)
                                                                    ctgcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGt                                                                            <  8:276142/148‑1 (MQ=255)
                                                                    ctgcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTAATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCAACCGTGCTGTa                                                                           >  1:275015/1‑149 (MQ=255)
                                                                      gcATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATg                                                                         >  1:75789/1‑149 (MQ=255)
                                                                        aTTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGcc                                                                       <  5:131344/149‑1 (MQ=255)
                                                                                cgccgcAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGt                                                               <  2:75789/149‑1 (MQ=255)
                                                                                  ccgcAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGc                                                             <  5:140204/149‑1 (MQ=255)
                                                                                         cATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            <  5:45439/111‑1 (MQ=255)
                                                                                         cATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCggg                                                                                            >  6:45439/1‑111 (MQ=255)
                                                                                          aTCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTg                                                     >  2:259270/1‑149 (MQ=255)
                                                                                               ttCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          <  1:316917/139‑1 (MQ=255)
                                                                                               ttCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          >  2:316917/1‑139 (MQ=255)
                                                                                                  gACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTACTACCGGTGCGGCGCTg                                             <  6:361277/149‑1 (MQ=255)
                                                                                                        cTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCAt                                        <  8:209284/148‑1 (MQ=255)
                                                                                                             ccTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATtcttct                                  <  1:259270/149‑1 (MQ=255)
                                                                                                               tgctgTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                >  2:278200/1‑149 (MQ=255)
                                                                                                                    ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                <  3:223097/144‑1 (MQ=255)
                                                                                                                    ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTgc                                >  4:223097/1‑144 (MQ=255)
                                                                                                                    ttCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCa                            <  5:166770/148‑1 (MQ=255)
                                                                                                                       aTTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          <  8:306861/115‑1 (MQ=255)
                                                                                                                       aTTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTAc                                                          >  7:306861/1‑115 (MQ=255)
                                                                                                                            ttcgatcaCCAGGCGCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatca                   >  3:786/5‑149 (MQ=255)
                                                                                                                            ttcgatcaCCAGGCGCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatca                   >  7:322870/5‑149 (MQ=255)
                                                                                                                                 tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatc                    >  1:41600/1‑143 (MQ=255)
                                                                                                                                 tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGtcatc                    <  2:41600/143‑1 (MQ=255)
                                                                                                                                 tcaCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTgg              <  2:275015/149‑1 (MQ=255)
                                                                                                                                   aCCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCAcccaggt            >  6:324543/1‑144 (MQ=255)
                                                                                                                                    cccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGt            >  7:30779/1‑148 (MQ=255)
                                                                                                                                     ccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATg                          >  2:268807/1‑133 (MQ=255)
                                                                                                                                     ccTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATg                          <  1:268807/133‑1 (MQ=255)
                                                                                                                                     caggcgCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGa          <  4:786/144‑1 (MQ=255)
                                                                                                                                        gcgCATGCCCAGGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGc       <  8:322870/147‑1 (MQ=255)
                                                                                                                                             tGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAt  <  8:30779/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TCGGCGACCAAGGCTTCACCTGGCTGTCCTTCCTGTTCATCGCCCTGCTCGGCAGCCTGATCGTAGTGCTGCATTTCACCCGCCGCAGCCATCACTTCGACGTGCTCAACCTGCTGTTCATTACCGTCATCACCCTGGGCATGCGCCTGGTGATCGAAGGCATCCCGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAAT  >  NC_002947/3911818‑3912107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑