Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,021,365	C→G	14.0%	*314S (TGA→TCA)	PP_3547 ←	NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,021,365	0	C	G	14.0%	86.8 / 8.0	36	*314S (TGA→TCA)	PP_3547	NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (19/10);  new base G (3/2);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

AAGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCGGGT  >  NC_002947/4021219‑4021512                                                                                                                                                   |                                                                                                                                                    aaGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAg                                                                                                                                                   >  5:92702/1‑149 (MQ=255) aaGGTGCTGTTGATCACCGGCTACGATAAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAg                                                                                                                                                   >  1:9099/1‑149 (MQ=255)    gTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAgcgc                                                                                                                                                <  2:176512/149‑1 (MQ=255)         gTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGcgcccgcc                                                                                                                                           >  3:252649/1‑149 (MQ=255)            gATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgtcaag                                                                                                                                        >  5:146205/1‑144 (MQ=255)             aTCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgtcaagg                                                                                                                                       <  6:92702/149‑7 (MQ=255)             aTCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgtcaagg                                                                                                                                       <  6:146205/149‑7 (MQ=255)                         gATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAgg                                                                                                                           >  6:271956/1‑149 (MQ=255)                                  gcggcgTTCCGCGACGGCCAACCGCTGCAAGGTAGGTTGGTGTGGACCAAGCCGTTCGAACTGCACCCACGGGCAGCGCGGGTTCGCTTGCTGCTGCAACCTTGCGGGGCGCCCACCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTg                                                                                                                  <  2:60616/149‑1 (MQ=255)                                                   ccAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTc                                                                                                 >  3:138692/1‑149 (MQ=255)                                                    cAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATagga                                                                                                          <  3:264174/139‑1 (MQ=255)                                                    cAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACATTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATagga                                                                                                          >  4:264174/1‑139 (MQ=255)                                                              aaGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAAc                                                                                       >  3:30453/1‑148 (MQ=255)                                                                 gTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCaga                                                                                   <  5:271956/149‑1 (MQ=255)                                                                      ttGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATg                                                                              >  1:320750/1‑149 (MQ=255)                                                                            gtcaCCAAGCCGTTCGAACTGCAAGCACGGGCAGCGGGGGGCCGCGAGGTGCGGTAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCg                                                                        <  4:236064/146‑1 (MQ=255)                                                                              gACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCGACCAGAGGATGGCCTCGGc                                                                      >  7:305546/1‑149 (MQ=255)                                                                              gACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGc                                                                      >  7:274912/1‑149 (MQ=255)                                                                                ccAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTc                                                                                                 <  5:338457/120‑1 (MQ=255)                                                                                ccAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTc                                                                                                 >  6:338457/1‑120 (MQ=255)                                                                                 cAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATAAGCCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCAc                                                                   >  1:109217/1‑149 (MQ=255)                                                                                    gCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTc                                                                >  4:82409/1‑149 (MQ=255)                                                                                                  aaGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGTTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGccccc                                                  >  6:93471/1‑149 (MQ=255)                                                                                                        tGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCa                                                                                     >  5:202090/1‑108 (MQ=255)                                                                                                             gtgggggTTGTCGAGCTGCTGGAACTTTGCGGGGCGCTATGGGCCCGCCGCCCAGGTCGATGAGGCTGCCTGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATg                                       <  2:109217/145‑1 (MQ=255)                                                                                                                  ggTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCaa                                  <  7:270413/149‑1 (MQ=255)                                                                                                                              ggtgGAACTTTTAGGGGCGCTCTGCGCCGGCCGCCCAGGTCTATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCGACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGa                       <  8:305546/146‑1 (MQ=255)                                                                                                                               ctgGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCACCCCTCGGCCCATGGGCCAGCCAGGTTCGacc                     >  6:248694/1‑147 (MQ=255)                                                                                                                                       ttGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATAAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGAc             >  1:51791/1‑149 (MQ=255)                                                                                                                                         ggcGGGCGCTGAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGAcgc           >  3:176939/3‑149 (MQ=255)                                                                                                                                         gccGGGCGCTACGCTCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATTTCCTCGGCCAGTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGAc             <  5:248694/145‑1 (MQ=255)                                                                                                                                             ggCGCTCCGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGTTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTc       <  5:93471/149‑1 (MQ=255)                                                                                                                                             ggCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTc       >  6:197537/1‑149 (MQ=255)                                                                                                                                                  tCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGa                       <  6:18072/128‑1 (MQ=255)                                                                                                                                                  tCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGa                       >  5:18072/1‑128 (MQ=255)                                                                                                                                                  tCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCGGGt  >  6:158208/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AAGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCGGGT  >  NC_002947/4021219‑4021512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑