Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,096,649	T→G	6.5%	S86A (TCG→GCG) ‡	PP_5608 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,096,649	0	T	G	6.5%	186.7 / 3.1	61	S86A (TCG→GCG) ‡	PP_5608	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/33);  new base G (2/2);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

CAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTCCAGC  >  NC_002947/4096505‑4096797                                                                                                                                                 |                                                                                                                                                     cAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTcgcg                                                                                                                                                  <  3:187630/149‑1 (MQ=255)  aGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCgg                                                                                                                                                 >  5:351886/1‑149 (MQ=255)   gATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGc                                                                                                                                                <  3:100437/149‑1 (MQ=255)       gccgccGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgt                                                                                                                                             <  3:40894/148‑3 (MQ=255)          gccgAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAATTTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGcc                                                                                                                                         <  1:109947/149‑1 (MQ=255)                gCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCagcagc                                                                                                                                   <  6:272405/149‑1 (MQ=255)                 cccAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCg                                                                                                                                  <  6:351886/149‑1 (MQ=255)                   cAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGAt                                                                                                                                <  2:62023/149‑1 (MQ=255)                      cAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACCCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCaa                                                                                                                             <  4:187632/149‑1 (MQ=255)                       aGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            >  2:57311/1‑149 (MQ=255)                        ggCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            >  3:59209/1‑148 (MQ=255)                         gCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCa                                                                                                                          <  1:256666/149‑1 (MQ=255)                           cAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGa                                                                                                                        >  6:37527/1‑149 (MQ=255)                            aaGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGAc                                                                                                                       <  4:76719/149‑1 (MQ=255)                             aGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACg                                                                                                                      <  4:141745/149‑1 (MQ=255)                               cTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGt                                                                                                                     >  3:322559/1‑148 (MQ=255)                               cTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgt                                                                                                                                          >  5:354154/1‑122 (MQ=255)                                    cGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTc                                                                                                                >  8:189994/1‑148 (MQ=255)                                     ggggTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGa                                                                                                              <  4:59209/149‑1 (MQ=255)                                           aCCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGt                                                                                                         <  1:57311/148‑1 (MQ=255)                                               tGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAGTTCCCCAGAGCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAAGCTACAACAGCGGCTCGCGGCTCTAGCCAGCAGCGAGCAAGCAGGCGTACTTCGAGCAGTGCGtt                                                                                                    >  4:217578/1‑149 (MQ=255)                                                agatctcACGCTCAATAAGCTGCAGGTACGCAAATTCCCCAAACCCTTGATGCTCATGACTGCCTTCGTCATTACCTAGCAAACCTACAACAGCCGCTCTCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGtt                                                                                                    <  7:316833/142‑1 (MQ=255)                                                   cGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAg                                                                                                 <  7:91095/148‑1 (MQ=255)                                                      cACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATg                                                                                             >  5:311485/1‑149 (MQ=255)                                                       aCGCGCAAGAAGCTGCAGGTACGCAAATTCCACAAACACTGGATGCTCATGACTGCCTTCGGCATTACCGAGCAAAAATACAACAGACGCTCAATGCTCAAGCAAGCAGCTATCAATCAGAAGAACTTCGATAAGTGCGTTCAgatag                                                                                             >  8:316833/1‑144 (MQ=255)                                                           gcAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGc                                                                                                                                          <  1:103204/99‑1 (MQ=255)                                                           gcAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGc                                                                                                                                          >  2:103204/1‑99 (MQ=255)                                                           gcAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGtt                                                                                        <  3:9459/149‑1 (MQ=255)                                                               gaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCaa                                                                                    <  6:311485/149‑1 (MQ=255)                                                                aagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCaa                                                                                                                             <  3:80687/107‑1 (MQ=255)                                                                aagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCaa                                                                                                                             >  4:80687/1‑107 (MQ=255)                                                                        ggTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGa                                                                                  <  4:153614/142‑1 (MQ=255)                                                                        ggTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGa                                                                                  >  3:153614/1‑142 (MQ=255)                                                                         gTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCg                                                                                                      >  4:175629/1‑121 (MQ=255)                                                                         gTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCg                                                                                                      <  3:175629/121‑1 (MQ=255)                                                                             gCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTg                                                                                       >  1:210919/1‑132 (MQ=255)                                                                             gCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTg                                                                                       <  2:210919/132‑1 (MQ=255)                                                                              cAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTa                                                                     <  8:310574/149‑1 (MQ=255)                                                                                 aTTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACtt                                                                  <  3:217578/149‑1 (MQ=255)                                                                                      ccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGATGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTAc                                                                    <  1:262437/142‑1 (MQ=255)                                                                                      ccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTAc                                                                    >  2:262437/1‑142 (MQ=255)                                                                                      ccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCAc                                                             >  5:281526/1‑149 (MQ=255)                                                                                       cAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACg                                                            >  3:136062/1‑149 (MQ=255)                                                                                          aCCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCt                                                         <  7:189994/149‑1 (MQ=255)                                                                                             cTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCa                                                      <  4:322559/149‑1 (MQ=255)                                                                                                     gcATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCg                                               >  4:267275/1‑148 (MQ=255)                                                                                                          aCTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAActct                                                                        <  7:8205/118‑1 (MQ=255)                                                                                                          aCTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAActct                                                                        >  8:8205/1‑118 (MQ=255)                                                                                                            tGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCg                                       >  6:45857/1‑149 (MQ=255)                                                                                                            tGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCACATCAGCg                                       >  5:224298/1‑149 (MQ=255)                                                                                                              ccTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGtc                                     <  5:37527/149‑1 (MQ=255)                                                                                                              ccTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGt                                      <  3:267275/148‑1 (MQ=255)                                                                                                                      aTTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCAt                                <  4:293100/146‑1 (MQ=255)                                                                                                                      aTTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCAt                                >  3:293100/1‑146 (MQ=255)                                                                                                                       ttACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAg                            <  6:281526/149‑1 (MQ=255)                                                                                                                                    cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGaa               <  5:45857/149‑1 (MQ=255)                                                                                                                                       caacaGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCACATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCg            <  6:224298/149‑1 (MQ=255)                                                                                                                                        ttgaGCCGCGAGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGaa               <  6:210456/142‑1 (MQ=255)                                                                                                                                        ttgaGCCGCGAGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGaa               >  5:210456/4‑145 (MQ=255)                                                                                                                                           aGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCt        >  6:18030/1‑149 (MQ=255)                                                                                                                                               gCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTc      >  5:20050/1‑147 (MQ=255)                                                                                                                                                 tCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTccagc  >  1:200082/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     CAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTCCAGC  >  NC_002947/4096505‑4096797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑