Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,274,413 G→T 9.6% V124L (GTG→TTG)  glcD → glycolate oxidase FAD‑linked subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,274,4130GT 9.6% 155.3 / 5.2 51V124L (GTG→TTG) glcDglycolate oxidase FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base T (3/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACG  >  NC_002947/4274267‑4274560
                                                                                                                                                  |                                                                                                                                                   
gCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGt                                                                                                                                                    <  2:96735/148‑1 (MQ=255)
 ccaccaGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTg                                                                                                                                                   >  7:187268/1‑148 (MQ=255)
       gcgcGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCgg                                                                                                                                            <  4:225632/149‑1 (MQ=255)
             cgTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGGTTGCACGCGGGCGTaa                                                                                                                                      <  8:181389/149‑2 (MQ=255)
              gTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACg                                                                                                                                     <  8:187268/149‑1 (MQ=255)
                        ttGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                           <  1:309820/149‑1 (MQ=255)
                        ttGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                           <  6:51739/149‑1 (MQ=255)
                              gTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTcc                                                                                                                     <  7:287181/149‑1 (MQ=255)
                                 gcgcCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGGTTGCACGCGGGCGTaa                                                                                                                                      <  1:114999/129‑2 (MQ=255)
                                 gcgcCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGGTTGCACGCGGGCGTaa                                                                                                                                      >  2:114999/1‑128 (MQ=255)
                                      ggTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                           <  6:159184/135‑1 (MQ=255)
                                      ggTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGcc                                                                                                                           >  5:159184/1‑135 (MQ=255)
                                                 gTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATg                                                                                                  >  7:290930/1‑149 (MQ=255)
                                                  tCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCAGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATg                                                                                                  <  5:69251/148‑1 (MQ=255)
                                                      gtggtgCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGt                                                                                                <  2:318888/146‑1 (MQ=255)
                                                         gtgCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGta                                                                                           >  4:14424/1‑148 (MQ=255)
                                                            cccTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGtactac                                                                                       >  6:20200/1‑149 (MQ=255)
                                                             ccTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCATCACCCGTATCCTCGAGGTCAACCCGCACGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCAATCACCCAGGCCGCCGCACCCCATGGAATGTCCTACg                                                                                      >  6:207091/1‑149 (MQ=255)
                                                                    cTGGGCAAGGGCCTCCTGCTGGTGTTGGCGCGCGTGAACCGGATGCGCGAGGTCAACCCGCAGGGGCGTTAAGCCCGCGTGGAACCGGGCGGAAGCAACCGGGCCATCTCCCAGGCCGCCGCCCCCCCGGGGATGTTCTACGCCCCCgg                                                                               >  2:212780/1‑148 (MQ=255)
                                                                     tGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGAc                                                                              >  2:196909/1‑149 (MQ=255)
                                                                       gCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGAccc                                                                            <  8:290930/149‑1 (MQ=255)
                                                                       gCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGAcc                                                                             <  3:14424/148‑1 (MQ=255)
                                                                        ccAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGAATCCTCGAGGTCACCCCGCAGGGGCGTTACGCCCGCGTGCAACAGGGCGTACGCTACCTGGCCAGATCCCAGGCCGCCGCCCCCCATCGAATGTACTCAGCACCCGAccca                                                                           >  6:287544/1‑148 (MQ=255)
                                                                         cAAGGGCATGCTTCGGGTGATGGCGCGGTTGAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGCCGTACGCAACCTg                                                                                                                              <  7:203830/97‑1 (MQ=255)
                                                                         cAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATg                                                                                                  <  4:255028/125‑1 (MQ=255)
                                                                                          tGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGc                                                         >  5:330901/1‑149 (MQ=255)
                                                                                                 gcgcTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCg                                                  <  6:330901/149‑1 (MQ=255)
                                                                                                 gcgcTTCAACAGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCAGATCgga                                                    >  5:200604/1‑145 (MQ=255)
                                                                                                 gcgcTTCAAACGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGc                                                             <  6:200604/138‑1 (MQ=255)
                                                                                                   gcTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGt                                                <  1:196909/149‑1 (MQ=255)
                                                                                                    cTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTg                                               <  5:20200/149‑1 (MQ=255)
                                                                                                          ccGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTg                                               <  1:239040/143‑1 (MQ=255)
                                                                                                          ccGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTg                                               >  2:239040/1‑143 (MQ=255)
                                                                                                          ccGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACg                                         >  6:312111/1‑149 (MQ=255)
                                                                                                           cGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGt                                        >  5:159245/1‑149 (MQ=255)
                                                                                                                ccTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATg                                                                                                  <  3:195065/86‑1 (MQ=255)
                                                                                                                ccTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATg                                                                                                  >  4:195065/1‑86 (MQ=255)
                                                                                                                 cTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCAt                                                                                                   <  8:151710/84‑1 (MQ=255)
                                                                                                                 cTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCAt                                                                                                   >  7:151710/1‑84 (MQ=255)
                                                                                                                  tCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTg                                                          >  5:250038/1‑124 (MQ=255)
                                                                                                                  tCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTg                                                          <  6:250038/124‑1 (MQ=255)
                                                                                                                             ccGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCCTCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTgg                      >  8:242125/1‑149 (MQ=255)
                                                                                                                                  ggggCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCt                                                        >  7:236179/1‑110 (MQ=255)
                                                                                                                                  ggggCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCt                                                        <  8:236179/110‑1 (MQ=255)
                                                                                                                                     gCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACt              >  4:313896/1‑149 (MQ=255)
                                                                                                                                       gTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCt                                                                       >  6:72382/1‑90 (MQ=255)
                                                                                                                                       gTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCt                                                                       <  5:72382/90‑1 (MQ=255)
                                                                                                                                        ttACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCAc                                                                                   <  2:62170/77‑1 (MQ=255)
                                                                                                                                        ttACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCAc                                                                                   >  1:62170/1‑77 (MQ=255)
                                                                                                                                        gtACGCCCGGTTGCACGCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGcc           >  4:252056/2‑149 (MQ=255)
                                                                                                                                        gtACGCCCGGTTGCACGCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGcc           >  8:318312/2‑149 (MQ=255)
                                                                                                                                                 cgTGGAACCGGGCGTACGCAACCTGGCCATCTCGCAGGCCGCCGCACCCCATGGTATGTACTAGGGACCCTACCCTTCCTCGCAAATTGCCTGCTCGATGGGTGGCAACGTCGCCTAAAACGCCGGTGGCGTGCACTGCCTCAAGTACg  <  5:207091/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCACCAGCGCGGCGTACCGGTGGTTGCGCGTGGCGCCGGTACCGGCCTGTCTGGTGGTGCCCTGCCGCTGGCCAAGGGCATCCTGCTGGTGATGGCGCGCTTCAACCGTATCCTCGAGGTCAACCCGCAGGGGCGTTACGCCCGCGTGCAACCGGGCGTACGCAACCTGGCCATCTCCCAGGCCGCCGCACCCCATGGTATGTACTACGCACCCGACCCTTCCTCGCAAATTGCCTGCTCGATCGGTGGCAACGTCGCCGAAAACGCCGGTGGCGTGCACTGCCTCAAGTACG  >  NC_002947/4274267‑4274560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑