Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,280,048 C→G 6.1% P416P (CCC→CCG)  PP_3750 → GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,280,0480CG 6.1% 209.5 / 3.7 66P416P (CCC→CCG) PP_3750GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base G (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

ACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGA  >  NC_002947/4279903‑4280188
                                                                                                                                                 |                                                                                                                                            
aCCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCggg                                                                                                                                           <  6:260090/149‑1 (MQ=255)
  cTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCgg                                                                                                                                            >  1:185715/1‑146 (MQ=255)
  cTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCgg                                                                                                                                            <  2:185715/146‑1 (MQ=255)
      aCAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAt                                                                                                                                     <  1:301849/149‑1 (MQ=255)
        aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGTGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                   >  1:98838/1‑149 (MQ=255)
        aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGTGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGAtgt                                                                                                                                   <  2:98838/149‑1 (MQ=255)
        aaGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCGGGGGTggccaa                                                                                                                                   <  3:43692/149‑5 (MQ=255)
               tCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTg                                                                                                                            >  5:93653/1‑149 (MQ=255)
               tCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTg                                                                                                                            >  4:305133/1‑149 (MQ=255)
                          cACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCt                                                                                                                 >  7:255934/1‑149 (MQ=255)
                           aCGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                        <  5:283120/141‑1 (MQ=255)
                           aCGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTgg                                                                                                                        >  6:283120/1‑141 (MQ=255)
                           aCGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTc                                                                                                                >  8:168270/1‑149 (MQ=255)
                           aCGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTc                                                                                                                >  3:83330/1‑149 (MQ=255)
                            cGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCt                                                                                                                             >  4:54533/1‑135 (MQ=255)
                            cGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCt                                                                                                                             <  3:54533/135‑1 (MQ=255)
                                gTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTcgcgcg                                                                                                           <  4:83330/149‑1 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCt                                                                                                                             >  7:158230/1‑128 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCt                                                                                                                             <  8:158230/128‑1 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGt                                                                                                          >  4:2713/1‑147 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGt                                                                                                          >  5:337596/1‑147 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGt                                                                                                          >  7:199021/1‑147 (MQ=255)
                                   tACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCA‑CCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAag                                                                                                       >  6:137042/1‑149 (MQ=255)
                                     cAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAagg                                                                                                      <  4:210930/149‑1 (MQ=255)
                                       gcgcACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggta                                                                                                    <  5:195871/149‑1 (MQ=255)
                                        cgcACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtag                                                                                                   <  6:93653/149‑1 (MQ=255)
                                                  gAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCt                                                                                         <  2:120556/149‑1 (MQ=255)
                                                    gcgcATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGt                                                                                       <  6:337596/149‑1 (MQ=255)
                                                        aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggtag                                                                                             >  5:286542/1‑139 (MQ=255)
                                                        aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggtag                                                                                             <  6:286542/139‑1 (MQ=255)
                                                        aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt                                                                                               <  7:151611/137‑1 (MQ=255)
                                                        aTCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAaggtagaggt                                                                                               >  8:151611/1‑137 (MQ=255)
                                                            tGGCGCGCCTGGGCGGCGATCTGGCAGCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGc                                                                                                                              >  1:9227/1‑102 (MQ=255)
                                                            tGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGc                                                                                                                              <  2:9227/102‑1 (MQ=255)
                                                              gcgcgcCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGc                                                                             <  3:2713/149‑1 (MQ=255)
                                                                       ggcggcGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCTGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCcttctt                                                                    <  7:168270/149‑1 (MQ=255)
                                                                          ggcgATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAg                                                                 >  6:105532/1‑149 (MQ=255)
                                                                             gATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGc                                                                >  3:151024/1‑147 (MQ=255)
                                                                                  ggCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGc                                                                <  7:199055/142‑1 (MQ=255)
                                                                                  ggCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGc                                                                >  8:199055/1‑142 (MQ=255)
                                                                                    cACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCg                                                       <  5:105532/149‑1 (MQ=255)
                                                                                         tGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACg                                                  >  8:176255/1‑149 (MQ=255)
                                                                                                 cgcCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGc                                          >  7:70547/1‑149 (MQ=255)
                                                                                                    cATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAt                                       >  6:312661/1‑149 (MQ=255)
                                                                                                     aTCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAtt                                      >  4:171954/1‑149 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCCCCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAtt                                      >  6:317373/1‑146 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACTGGTACGGCCGGGGCTATTGAt                                   >  3:185939/1‑149 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTAtt                                      <  5:317373/146‑1 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                   >  1:196304/1‑149 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                   >  2:328115/1‑149 (MQ=255)
                                                                                                        cccGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCA‑CCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                   <  5:137042/148‑1 (MQ=255)
                                                                                                                      cTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCa                     <  7:176255/149‑1 (MQ=255)
                                                                                                                       ttCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCa                     >  5:168853/1‑148 (MQ=255)
                                                                                                                       ttCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCa                     >  5:163804/1‑148 (MQ=255)
                                                                                                                         ccACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCa                     <  6:168853/146‑1 (MQ=255)
                                                                                                                         ccACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCa                     <  6:163804/146‑1 (MQ=255)
                                                                                                                           aCCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGaa                <  1:328115/149‑1 (MQ=255)
                                                                                                                             cTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGa                                                              >  6:176905/1‑101 (MQ=255)
                                                                                                                                  tGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTgg         <  2:196304/149‑1 (MQ=255)
                                                                                                                                    ccTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCAt                    <  2:276273/136‑1 (MQ=255)
                                                                                                                                      ttGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                   >  1:127976/1‑119 (MQ=255)
                                                                                                                                      ttGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGAt                                   <  2:127976/119‑1 (MQ=255)
                                                                                                                                      acatccACCCCGGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGatat     >  2:346899/5‑149 (MQ=255)
                                                                                                                                         gCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGa  <  5:312661/149‑1 (MQ=255)
                                                                                                                                          ccACCCCGGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGatat     >  6:273234/1‑145 (MQ=255)
                                                                                                                                          ccACCCCGGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGatat     <  5:273234/145‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ACCTGAACAAGCACATCCGCCGCTGCCACGAGGTCTACAGCGCACGCCGCGAGCGCATCCTGGCGCGCCTGGGCGGCGATCTGGCACCGTGGTTCAGCGCCATCCCCGCCAGCGCAGGCTTCCACCTCAGTGCCTTGGCCACCCCCGGGGTGGATGTGGAGCTGTTGGCCAACCTCGCGCGTAAGGTAGAGGTAGGCCTGTATTCACTGGCACCCTTCTTCAGCGAGGCACCGGTACGGCCGGGGCTATTGATGGGCTTCGGCGCCATCGAACTGCTGGATATCGA  >  NC_002947/4279903‑4280188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑