Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,297,049 C→G 8.9% T40S (ACC→AGC)  PP_3767 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,297,0490CG 8.9% 137.3 / 4.5 45T40S (ACC→AGC) PP_3767hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/19);  new base G (2/2);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

CTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCTGTACCCCCCT  >  NC_002947/4296903‑4297196
                                                                                                                                                  |                                                                                                                                                   
cTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCacac                                                                                                                                                     <  7:87148/147‑1 (MQ=255)
cTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACAcc                                                                                                                                                    <  8:244396/148‑1 (MQ=255)
    acaTACCACAGCGAGGTAAGGAAGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGc                                                                                                                                                 >  7:53399/1‑147 (MQ=255)
                   gTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGcc                                                                                                                                >  4:144997/1‑149 (MQ=255)
                                       tCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGAcc                                                                                                            >  3:300052/1‑149 (MQ=255)
                                        cACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGAcc                                                                                                            <  4:300052/148‑1 (MQ=255)
                                         aCTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCtt                                                                                                          >  7:232821/1‑149 (MQ=255)
                                                aTACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATg                                                                                                                            >  1:81529/1‑124 (MQ=255)
                                                aTACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATg                                                                                                                            <  2:81529/124‑1 (MQ=255)
                                                          cacaCCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCgtgt                                                                                         <  1:148975/149‑1 (MQ=255)
                                                             acCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCtt                                                                                                          >  6:110773/1‑129 (MQ=255)
                                                             acCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCtt                                                                                                          <  5:110773/129‑1 (MQ=255)
                                                               cATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGCCTGCCTGATCACGCCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTATTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGc                                                                                    >  7:179177/1‑149 (MQ=255)
                                                                 tCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTg                                                                                  <  8:232821/149‑1 (MQ=255)
                                                                 tCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTg                                                                                  >  2:3942/1‑149 (MQ=255)
                                                                     tGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGcc                                                                               >  4:236190/1‑148 (MQ=255)
                                                                        cAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGAc                                                                                                             <  6:237510/115‑1 (MQ=255)
                                                                        cAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGAc                                                                                                             >  5:237510/1‑115 (MQ=255)
                                                                                tGCCCCATCCCGACCCACCCCAGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCa                                                                   <  8:179177/149‑1 (MQ=255)
                                                                                   cccATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACAGCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTg                                                                >  6:252643/1‑149 (MQ=255)
                                                                                   cccATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACAGCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTg                                                                >  6:248226/1‑149 (MQ=255)
                                                                                      aTCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTTAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTc                                                             >  5:43016/1‑149 (MQ=255)
                                                                                              tCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACaaa                                                       >  5:232671/1‑147 (MQ=255)
                                                                                                aCCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAAc                                                      <  1:110211/146‑1 (MQ=255)
                                                                                                aCCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAAc                                                      >  2:110211/1‑146 (MQ=255)
                                                                                                  cccTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTACGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTg                                                 >  1:342256/1‑149 (MQ=255)
                                                                                                   ccTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTgg                                                <  3:236190/149‑1 (MQ=255)
                                                                                                         cTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACAGCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACg                                          <  5:248226/149‑1 (MQ=255)
                                                                                                         cTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACAGCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACg                                          <  5:252643/149‑1 (MQ=255)
                                                                                                                        ccTGATCACGGCACCTGACGGGCACACCAGCAAGCGTACGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTc                            <  2:342256/148‑1 (MQ=255)
                                                                                                                         cTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCt                           >  5:122256/1‑148 (MQ=255)
                                                                                                                          tGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTg                                                                                  >  6:107736/1‑92 (MQ=255)
                                                                                                                          tGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTg                                                                                  <  5:107736/92‑1 (MQ=255)
                                                                                                                           gATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGAt                        >  7:313650/1‑149 (MQ=255)
                                                                                                                            aTCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATc                       <  6:232671/149‑1 (MQ=255)
                                                                                                                             tCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATcc                      <  6:122256/149‑1 (MQ=255)
                                                                                                                               aCGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACg                                          <  5:357725/127‑1 (MQ=255)
                                                                                                                               aCGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACg                                          >  6:357725/1‑127 (MQ=255)
                                                                                                                                    aCCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCg               >  8:333125/1‑149 (MQ=255)
                                                                                                                                     ccTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGc              <  7:333125/149‑1 (MQ=255)
                                                                                                                                     ccTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGc              <  1:3942/149‑1 (MQ=255)
                                                                                                                                        gACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCt                                                  <  4:311586/110‑1 (MQ=255)
                                                                                                                                        gACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCt                                                  >  2:257761/1‑110 (MQ=255)
                                                                                                                                        gACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCt                                                  <  1:257761/110‑1 (MQ=255)
                                                                                                                                         aCGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCt            <  8:313650/147‑1 (MQ=255)
                                                                                                                                         aCGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCTg           >  5:48207/1‑148 (MQ=255)
                                                                                                                                             gCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCTGTAccc      >  2:315476/1‑149 (MQ=255)
                                                                                                                                                 acCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCTGTACCCCCCt  >  4:103675/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTTCACATACCACAGCGAGGTAAGGACGATGCAATCTCTTCACTGTGAATACCGCGAGCACACCATCACTGCCAGCGTGATGCCCCATCCCGACTCACCCCTGCCCTATGCCGCCGGCTGCCTGATCACGGCACCTGACGGGCACACCAGCAAGCGTATGTCGATGCCCATGCAATTCTTCTCCGACCTTGAAAATGCGCAGCGTGTGTCGCTGGCCCATGGCCGCGCACTGGTCGACAAACAGCTGGATAACGGGCACGAGGTGTTCTGATCCGGTTTCGCCTGTACCCCCCT  >  NC_002947/4296903‑4297196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑