Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,356,298 G→C 8.9% A272A (GCG→GCC)  modC → molybdate ABC transporter ATP‑binding protein ModC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,356,2980GC 8.9% 131.4 / 4.0 45A272A (GCG→GCC) modCmolybdate ABC transporter ATP‑binding protein ModC
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAT  >  NC_002947/4356155‑4356442
                                                                                                                                               |                                                                                                                                                
gCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACgcggcg                                                                                                                                               >  4:56589/1‑147 (MQ=255)
gCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCa                                                                                                                                             <  4:39775/149‑1 (MQ=255)
 cTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                                                                                                                                            >  1:14439/1‑149 (MQ=255)
 cTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAg                                                                                                                                            <  1:272609/149‑1 (MQ=255)
  tCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAgg                                                                                                                                           >  3:147551/1‑149 (MQ=255)
   cGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGt                                                                                                                                          <  1:14677/149‑1 (MQ=255)
            cTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATg                                                                                                                                      <  6:66449/144‑1 (MQ=255)
            cTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATg                                                                                                                                      >  5:66449/1‑144 (MQ=255)
                 gCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCAccc                                                                                                                            >  2:193047/1‑149 (MQ=255)
                       ccAGGGCGACGCCGCCGGCGTCGTGTTAGAAGGTAGGGTGGTTGGGCACGCCCCGCATTAAGGCCTGCTGGACCTGCGCCTGCCCGGCAGCACCGGGCCGCGGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCCGCACCCAGcgcg                                                                                                                      >  8:113701/1‑149 (MQ=255)
                       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGcgcg                                                                                                                      >  7:125161/1‑149 (MQ=255)
                       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCCCATCACCTGCGCCGCGTGGGTGATGCgcag                                                                                                                                 <  4:265798/138‑1 (MQ=38)
                       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCCCATCACCTGCGCCGCGTGGGTGATGCgcag                                                                                                                                 >  3:265798/1‑138 (MQ=38)
                                 gacgCCGGCGTCGTGTTCGAAGGTAGGGGGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCa                                                                                                            <  4:35929/149‑1 (MQ=255)
                                   cgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgc                                                                                                                                   <  3:236234/124‑1 (MQ=255)
                                   cgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgc                                                                                                                                   >  4:236234/1‑124 (MQ=255)
                                    gCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGc                                                                                                         >  3:244640/1‑149 (MQ=255)
                                      cGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGccc                                                                                                       >  8:269514/1‑149 (MQ=255)
                                       ggCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCg                                                                                                      <  4:244640/149‑1 (MQ=255)
                                             gtgtTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCTGCGCCGCGTGGGTGATGCgcagca                                                                                                                               <  1:334015/118‑1 (MQ=38)
                                             gtgtTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCTGCGCCGCGTGGGTGATGCgcagca                                                                                                                               >  2:334015/1‑118 (MQ=38)
                                                    aaGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTg                                                                                         >  5:79690/1‑149 (MQ=255)
                                                     aGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTgg                                                                                        <  4:147551/149‑1 (MQ=255)
                                                                     cACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAg                                                                        >  6:8122/1‑149 (MQ=255)
                                                                           ccGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACt                                                                                    <  1:259164/131‑1 (MQ=255)
                                                                           ccGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACt                                                                                    >  2:259164/1‑131 (MQ=255)
                                                                             gCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                <  5:8122/149‑1 (MQ=255)
                                                                               aTTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGc                                                              <  7:269514/149‑1 (MQ=255)
                                                                                   cGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATc                                                           <  3:56589/148‑1 (MQ=255)
                                                                                          ctgGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAAcc                                                    <  5:100636/148‑1 (MQ=255)
                                                                                                     ccTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGc                                        <  6:79690/149‑1 (MQ=255)
                                                                                                     ccTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGc                                        <  7:113701/149‑1 (MQ=255)
                                                                                                        gCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCgtg                                     >  8:213805/1‑149 (MQ=255)
                                                                                                          ccGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATGGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGtgtg                                   >  1:47389/1‑149 (MQ=255)
                                                                                                             gcagcaGCGGGCCGCTGCTGCGCATCAGCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATATCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTgcgc                                <  8:125161/149‑1 (MQ=255)
                                                                                                             gcagcaGCGGCCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGTATCCTCAACCGCCTGCCGGTGCGTGTgcgc                                >  7:254366/1‑149 (MQ=255)
                                                                                                                          gctgctGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCaa                                                      <  4:261512/114‑1 (MQ=255)
                                                                                                                          gctgctGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCaa                                                      >  3:261512/1‑114 (MQ=255)
                                                                                                                                 cgcATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATGGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGcc              <  2:47389/147‑1 (MQ=255)
                                                                                                                                       aCCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGgc      >  1:59641/1‑149 (MQ=255)
                                                                                                                                        cccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCAGGCGGCCAACCCGgcg     >  5:156504/1‑149 (MQ=255)
                                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                >  2:74189/1‑89 (MQ=255)
                                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                <  1:74189/89‑1 (MQ=255)
                                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGgcg     >  3:257744/1‑148 (MQ=255)
                                                                                                                                           aCGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGTATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAt  <  8:254366/149‑1 (MQ=255)
                                                                                                                                           aCGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAt  <  4:257744/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCTCGCCTTGACCTGTCGCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAT  >  NC_002947/4356155‑4356442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑