Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,356,311	G→C	10.0%	G277R (GGC→CGC)	modC →	molybdate ABC transporter ATP‑binding protein ModC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,356,311	0	G	C	10.0%	115.4 / 3.4	40	G277R (GGC→CGC)	modC	molybdate ABC transporter ATP‑binding protein ModC
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base C (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

GCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCATGTGCTGGTCAGCCTG  >  NC_002947/4356172‑4356457                                                                                                                                            |                                                                                                                                                   gCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCAccc                                                                                                                                           >  2:193047/1‑149 (MQ=255)       ccAGGGCGACGCCGCCGGCGTCGTGTTAGAAGGTAGGGTGGTTGGGCACGCCCCGCATTAAGGCCTGCTGGACCTGCGCCTGCCCGGCAGCACCGGGCCGCGGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCCGCACCCAGcgcg                                                                                                                                     >  8:113701/1‑149 (MQ=255)       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGcgcg                                                                                                                                     >  7:125161/1‑149 (MQ=255)       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCCCATCACCTGCGCCGCGTGGGTGATGCgcag                                                                                                                                                <  4:265798/138‑1 (MQ=38)       ccAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCCCATCACCTGCGCCGCGTGGGTGATGCgcag                                                                                                                                                >  3:265798/1‑138 (MQ=38)                 gacgCCGGCGTCGTGTTCGAAGGTAGGGGGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCa                                                                                                                           <  4:35929/149‑1 (MQ=255)                   cgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgc                                                                                                                                                  >  4:236234/1‑124 (MQ=255)                   cgCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGgc                                                                                                                                                  <  3:236234/124‑1 (MQ=255)                    gCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGc                                                                                                                        >  3:244640/1‑149 (MQ=255)                      cGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGccc                                                                                                                      >  8:269514/1‑149 (MQ=255)                       ggCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCg                                                                                                                     <  4:244640/149‑1 (MQ=255)                             gtgtTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCTGCGCCGCGTGGGTGATGCgcagca                                                                                                                                              <  1:334015/118‑1 (MQ=38)                             gtgtTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCTGCGCCGCGTGGGTGATGCgcagca                                                                                                                                              >  2:334015/1‑118 (MQ=38)                                    aaGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTg                                                                                                        >  5:79690/1‑149 (MQ=255)                                     aGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTgg                                                                                                       <  4:147551/149‑1 (MQ=255)                                                     cACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAg                                                                                       >  6:8122/1‑149 (MQ=255)                                                           ccGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACt                                                                                                   >  2:259164/1‑131 (MQ=255)                                                           ccGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACt                                                                                                   <  1:259164/131‑1 (MQ=255)                                                             gCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                               <  5:8122/149‑1 (MQ=255)                                                               aTTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGc                                                                             <  7:269514/149‑1 (MQ=255)                                                                   cGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATc                                                                          <  3:56589/148‑1 (MQ=255)                                                                          ctgGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAAcc                                                                   <  5:100636/148‑1 (MQ=255)                                                                                     ccTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGc                                                       <  7:113701/149‑1 (MQ=255)                                                                                     ccTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGc                                                       <  6:79690/149‑1 (MQ=255)                                                                                        gCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCgtg                                                    >  8:213805/1‑149 (MQ=255)                                                                                          ccGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATGGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGtgtg                                                  >  1:47389/1‑149 (MQ=255)                                                                                             gcagcaGCGGGCCGCTGCTGCGCATCAGCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATATCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTgcgc                                               <  8:125161/149‑1 (MQ=255)                                                                                             gcagcaGCGGCCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGTATCCTCAACCGCCTGCCGGTGCGTGTgcgc                                               >  7:254366/1‑149 (MQ=255)                                                                                                          gctgctGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCaa                                                                     <  4:261512/114‑1 (MQ=255)                                                                                                          gctgctGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCaa                                                                     >  3:261512/1‑114 (MQ=255)                                                                                                                 cgcATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATGGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGcc                             <  2:47389/147‑1 (MQ=255)                                                                                                                       aCCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGgc                     >  1:59641/1‑149 (MQ=255)                                                                                                                        cccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCAGGCGGCCAACCCGgcg                    >  5:156504/1‑149 (MQ=255)                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                               <  1:74189/89‑1 (MQ=255)                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGa                                                                               >  2:74189/1‑89 (MQ=255)                                                                                                                         ccACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGgcg                    >  3:257744/1‑148 (MQ=255)                                                                                                                           aCGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGTATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAt                 <  8:254366/149‑1 (MQ=255)                                                                                                                           aCGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCAt                 <  4:257744/149‑1 (MQ=255)                                                                                                                                        gATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCATGTGCTGGTCAGcc    >  3:318747/1‑149 (MQ=255)                                                                                                                                         aTGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCATGTGCTGGTCAGCCt   <  4:318747/149‑1 (MQ=255)                                                                                                                                           gggCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCATGTGCTGGTCAGCCTg  >  8:58020/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                                   GCTGGCCCAGGGCGACGACGCCGGCGTCGTGTTCGAAGGTAGGGTGGTTGGGCACGACCCGCATTACGGCCTGCTGGACCTGCGCCTGCCCGGCAGCAGCGGGCCGCTGCTGCGCATCACCCACGCGGCGCAGGTGATGGGCAGCACCCTGCGCGTCAAGGTGCAGGCCCGGGATGTCAGCCTGGCACTGGCGGCGGATAGCGCATCGAGCATCCTCAACCGCCTGCCGGTGCGTGTGCGCGAGAGCTGCCCGGCGGCCAACCCGGCGCATGTGCTGGTCAGCCTG  >  NC_002947/4356172‑4356457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑