Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 414,921 G→A 8.9% A247T (GCC→ACC)  waaF → ADP‑heptose:LPS heptosyltransferase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947414,9210GA 8.9% 125.2 / 2.3 45A247T (GCC→ACC) waaFADP‑heptose:LPS heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

CGCTGTGGCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAACCGTCCGTTGGTGGCGGTGTACGGCTCGACTTCACCCGGCTTCACCCCGCCGCTGGCCGAACAG  >  NC_002947/414773‑415067
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cGCTGTGGCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTgg                                                                                                                                                    >  4:175641/1‑149 (MQ=255)
  cTGTGGCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGcc                                                                                                                                                  <  6:64973/149‑1 (MQ=255)
     tgGCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAg                                                                                                                                               <  3:204192/149‑1 (MQ=255)
       gCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCAGCGAGGTCTCACCGGccaggt                                                                                                                                              <  2:237348/148‑5 (MQ=255)
              cgatgATTCGCCAAGGCTGGAAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGAc                                                                                                                                      <  3:244637/149‑1 (MQ=255)
               gatgatTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGAc                                                                                                                                      <  6:153631/148‑1 (MQ=255)
               gatgatTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGAc                                                                                                                                      >  5:153631/1‑148 (MQ=255)
                        ccAAGGCTGGCAAGTGTGGCTGTTTGGTTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGAc                                                                                                                                      <  7:104934/139‑1 (MQ=255)
                        ccAAGGCTGGCAAGTGTGGCTGTTTGGTTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGAc                                                                                                                                      >  8:104934/1‑139 (MQ=255)
                        ccAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCt                                                                                                                            >  7:35980/1‑149 (MQ=255)
                        ccAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCt                                                                                                                            >  5:254359/1‑149 (MQ=255)
                        ccAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCt                                                                                                                            <  4:299554/149‑1 (MQ=255)
                                      tgtgGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACGTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCgtg                                                                                                              <  1:234063/149‑1 (MQ=255)
                                        tgGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGc                                                                                                                                             >  6:105225/1‑116 (MQ=255)
                                         ggCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCgtggt                                                                                                            <  4:179973/148‑1 (MQ=255)
                                                    tCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGa                                                                                                                                  <  2:284011/115‑1 (MQ=255)
                                                    tCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGa                                                                                                                                  >  1:284011/1‑115 (MQ=255)
                                                    tCGAAGAACGAGCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTc                                                                                                <  5:249397/149‑1 (MQ=255)
                                                     cGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCAGCGAGGTCTCACCGGccaggtt                                                                                                                                             >  2:52420/1‑98 (MQ=37)
                                                     cGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCAGCGAGGTCTCACCGGccaggtt                                                                                                                                             <  1:52420/103‑6 (MQ=37)
                                                      gaagaaCGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCg                                                                                              <  3:175641/149‑1 (MQ=255)
                                                         gaaCGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGcc                                                                                           <  1:139170/149‑1 (MQ=255)
                                                          aaCGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCgtgg                                                                                                             <  6:66059/130‑1 (MQ=255)
                                                          aaCGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCt                                                                                          >  6:130541/1‑149 (MQ=255)
                                                          aaCGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTCGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCgtgg                                                                                                             >  5:66059/1‑130 (MQ=255)
                                                            cGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGa                                                                                        >  2:100120/1‑149 (MQ=255)
                                                                 aCCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCAc                                                                                   <  6:254359/149‑1 (MQ=255)
                                                                  cccGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGCCTCCGGCCTGATGCACg                                                                                  >  5:200283/1‑149 (MQ=255)
                                                                  cccGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACg                                                                                  >  3:148358/1‑149 (MQ=255)
                                                                          gtGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCcgct                                                                          >  4:41346/1‑149 (MQ=255)
                                                                             aGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATg                                                                                                                                >  2:54588/1‑92 (MQ=255)
                                                                             aGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATg                                                                                                                                <  1:54588/92‑1 (MQ=255)
                                                                              gCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCcgctgcgc                                                                      <  4:148358/149‑1 (MQ=255)
                                                                                 gATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGACCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCACCGACTCCGGc                                                                                            >  6:211105/1‑124 (MQ=255)
                                                                                 gATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGACCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGc                                                                                            <  5:211105/124‑1 (MQ=255)
                                                                                 gATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGACCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGc                                                                                            <  5:206047/124‑1 (MQ=255)
                                                                                 gATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGACCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGc                                                                                            >  6:206047/1‑124 (MQ=255)
                                                                                      gcgACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCTCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAAccgt                                                              <  1:100120/149‑1 (MQ=255)
                                                                                      gcgACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGaa                                                                  >  6:176680/1‑145 (MQ=255)
                                                                                      gcgACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGaa                                                                  <  5:176680/145‑1 (MQ=255)
                                                                                        gACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAAccgtcc                                                            <  3:41346/149‑1 (MQ=255)
                                                                                               tGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAACCGTCCGTtggtg                                                     >  7:268/1‑149 (MQ=255)
                                                                                                                                           aCCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCACTGCGCTGAACCGTCCGTTGGTGGCGGTGTACGGCTCGACTTCAccc                             <  5:322629/129‑1 (MQ=255)
                                                                                                                                           aCCTCGCTGGACGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGAAGCACCTGGCCACTGCGCTGAACCGACCGTTGGTGCCGGTGTACGGCTCGACTTCcccc                             >  6:322629/1‑129 (MQ=255)
                                                                                                                                             cTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAACCGTCCGTTGGTGGCGGTGTACGGCTCGACTTCACCCGGCTTCACCCCGCCGCTGGcc        >  7:189024/1‑148 (MQ=255)
                                                                                                                                                  tGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAACCGTCCGTTGGTGGCGGTGTACGGCTCGACTTCACCCGGCTTCACCCCGCCGCTGGCCGAACAg  >  4:61532/1‑149 (MQ=255)
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CGCTGTGGCCGATGCGATGATTCGCCAAGGCTGGCAAGTGTGGCTGTTTGGCTCGAAGAACGATCACCCGGTCGGTGAGCAGATCCGCGACCGGCTGATTCCGGGGCTCCGTGAAGAATCGTCCAACCTGGCCGGTGAGACCTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCCATGCCGTGGTGTCCAACGACTCCGGCCTGATGCACGTGGCCGCTGCGCTGAACCGTCCGTTGGTGGCGGTGTACGGCTCGACTTCACCCGGCTTCACCCCGCCGCTGGCCGAACAG  >  NC_002947/414773‑415067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑