| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,370,345 | T→C | 7.5% | A13A (GCT→GCC) | PP_3847 → | LuxR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,370,345 | 0 | T | C | 7.5% | 146.5 / 2.8 | 53 | A13A (GCT→GCC) | PP_3847 | LuxR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (25/24); new base C (2/2); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01 | |||||||||||
CACGTGTATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTCAGCAGTGGTCGCCCGCAGGTGCTCGCTGAAT > NC_002947/4370199‑4370493 | cACGTGTATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCtt < 7:29496/148‑1 (MQ=255) gtgtATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCtt < 8:197537/145‑1 (MQ=255) gtgtATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCtt > 7:197537/1‑145 (MQ=255) gtgtATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCa > 4:301679/1‑149 (MQ=255) ggCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCAt > 6:284826/1‑149 (MQ=255) aaTCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCa < 3:301679/149‑1 (MQ=255) gCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGGAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGAc > 2:181362/1‑148 (MQ=255) gCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGAc > 3:243819/1‑148 (MQ=255) ccAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACt > 6:295101/1‑148 (MQ=255) aaaCTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCCTGGTGCCAAGCCAtgtc > 7:47911/1‑137 (MQ=255) aaaCTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCCTGGTGCCAAGCCAtgtc < 8:47911/140‑4 (MQ=255) aaCTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATc > 7:139334/1‑149 (MQ=255) cTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGa < 5:284826/149‑1 (MQ=255) tGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGACAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGACCAT‑‑TCCACTGCAGGA‑‑‑CATGACTTGGCACCAGGCCATCGGCACACTAATCTAg > 6:211936/1‑149 (MQ=255) ccGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGAcc > 8:176558/1‑149 (MQ=255) ccGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATAACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGAcc > 2:108595/1‑149 (MQ=255) gCCAGGTCTGAAACTAAGTATGTAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGTAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑GATGACTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGc < 5:211936/149‑1 (MQ=255) tGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTgg < 8:59181/102‑1 (MQ=255) aaCTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTct < 7:130764/148‑1 (MQ=255) cTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCCTGGTGCCAAGCCAtgtc > 2:311365/1‑108 (MQ=255) cTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCCTGGTGCCAAGCCAtgtc < 1:311365/111‑4 (MQ=255) aGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCg > 3:216286/1‑119 (MQ=255) aGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCg < 4:216286/119‑1 (MQ=255) aGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTctggctg < 6:181341/148‑1 (MQ=255) gagaCCCGTAACACGGAAAGTAATTACTTCGATGGGTTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTgg < 3:110235/149‑1 (MQ=255) agagCCGTAAAACGGTAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTgg < 1:181362/148‑1 (MQ=255) ccGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTa < 5:131149/104‑1 (MQ=255) ccGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTa > 6:131149/1‑104 (MQ=255) ccGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGt > 8:70545/1‑149 (MQ=255) tAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATTGGTAGAAACGGGAGCATTCTACACTGCAGGATCCCCCTGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGc < 1:108595/149‑1 (MQ=255) cGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGga < 6:187973/149‑1 (MQ=255) ggAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGgat < 8:139334/149‑1 (MQ=255) gTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGgatagata < 5:295101/149‑1 (MQ=255) tAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGga > 4:39298/1‑142 (MQ=255) tAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGga < 3:39298/142‑1 (MQ=255) ttCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATc < 5:317473/85‑1 (MQ=255) ttCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATc > 6:317473/1‑85 (MQ=255) gATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGgataga > 6:10101/1‑136 (MQ=255) gATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTg < 7:176558/148‑1 (MQ=255) tGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTctgg < 8:289260/107‑1 (MQ=255) tGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTctgg > 7:289260/1‑107 (MQ=255) cccGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGTACTTGACCGCCCTAATTTct > 6:345072/1‑96 (MQ=255) cccGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGTACTTGACCGCCCTAATTTct < 5:345072/96‑1 (MQ=255) gTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGata > 3:195375/1‑149 (MQ=255) tAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGa < 2:315326/55‑1 (MQ=255) tAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGa > 1:315326/1‑55 (MQ=255) aCGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTcag > 1:55800/1‑148 (MQ=255) gggAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGTCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGTGTCGTGCTGATATTcagaag > 4:59604/1‑149 (MQ=255) aGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTcagcag < 1:29737/146‑1 (MQ=255) aGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTcagcag > 2:29737/1‑146 (MQ=255) t‑‑tACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTCAGCAGTGGTCGc > 3:141288/1‑149 (MQ=255) tGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTCAGCAGTGGTCGCCCGCAGGTGCTCGCt < 4:195375/149‑1 (MQ=255) gCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTca > 5:192761/1‑121 (MQ=255) gCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTca > 3:151912/1‑121 (MQ=255) gCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTca < 4:151912/121‑1 (MQ=255) gCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTca < 6:192761/121‑1 (MQ=255) ttGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTCAGCAGTGGTCGCCCGCACGTGCTCGCTGAAt > 8:223354/1‑149 (MQ=255) | CACGTGTATGTTGGCTCAATCGCCAAACTGAACAGCACCCGCCAGGGCTGAAACTAAGTATGGAGAGCCGTAAAACGGAAAGTAATTACTTCGATGGGGTAACCCCGTATGGGTAGAAACGGGAGCAT‑‑TACACTGCAGGA‑‑‑CATGGCTTGGCACCAGGCCATCGGCAGACTAATCGAGGCACTTGACCGCCCTAATTTCTGGCTGGCCTTGGTGCGTCAACTGGATAGATACATTCCGGTGGACAGTTGGGTCGTGCTGATATTCAGCAGTGGTCGCCCGCAGGTGCTCGCTGAAT > NC_002947/4370199‑4370493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |