Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,379,142	G→A	9.1%	A114A (GCG→GCA)  A66V (GCG→GTG) ‡	PP_5639 → PP_3855 ←	hypothetical protein hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,379,142	0	G	A	9.1%	184.6 / 6.0	66	A114A (GCG→GCA)  A66V (GCG→GTG) ‡	PP_5639 PP_3855	hypothetical protein hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base A (3/3);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCTGTT  >  NC_002947/4379008‑4379290                                                                                                                                       |                                                                                                                                                     tACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCg                                                                                                                                          >  1:70603/1‑147 (MQ=255)   cGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGc                                                                                                                                      <  3:208493/149‑1 (MQ=255)         tCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCg                                                                                                                                 >  7:148394/1‑148 (MQ=255)           tGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCaa                                                                                                                              >  4:239616/1‑149 (MQ=255)             aTCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGc                                                                                                                                      <  3:93157/139‑1 (MQ=255)             aTCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAAt                                                                                                                             >  8:34655/1‑148 (MQ=255)             aTCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTACCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGc                                                                                                                                      >  1:323666/1‑139 (MQ=255)             aTCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTACCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGc                                                                                                                                      <  2:323666/139‑1 (MQ=255)              tCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGa                                                                                                                                        <  6:75275/136‑1 (MQ=255)              tCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGa                                                                                                                                        >  5:75275/1‑136 (MQ=255)                gACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGAt                                                                                                                          <  7:26137/148‑1 (MQ=255)                    tGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGaca                                                                                                                     <  3:260551/149‑1 (MQ=255)                          cGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTa                                                                                                                <  3:239616/148‑1 (MQ=255)                          cGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTa                                                                                                                <  8:148394/148‑1 (MQ=255)                          cGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTa                                                                                                                <  5:253014/148‑1 (MQ=255)                           gTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAgc                                                                                                              >  1:39149/1‑149 (MQ=255)                              tGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCa                                                                                                                                                    >  5:179061/1‑108 (MQ=255)                              tGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCa                                                                                                                                                    <  6:179061/108‑1 (MQ=255)                                         acacAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  <  6:297379/147‑1 (MQ=255)                                         acacAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  <  2:70603/147‑1 (MQ=255)                                         acacAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTc                                                                                                 <  2:39149/148‑1 (MQ=255)                                             aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCGGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTa                                                                                                                <  4:89924/129‑1 (MQ=255)                                             aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTa                                                                                                                >  3:89924/1‑129 (MQ=255)                                             aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCa                                                                                                >  7:271321/1‑145 (MQ=255)                                             aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCa                                                                                                <  8:271321/145‑1 (MQ=255)                                             aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTAc                                                                                            >  2:122208/1‑149 (MQ=255)                                                          tACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  >  8:24287/1‑130 (MQ=255)                                                          tACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  <  7:24287/130‑1 (MQ=255)                                                             acCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATAcc                                                                            >  2:14537/1‑149 (MQ=255)                                                               cAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTg                                                                          >  5:338282/1‑149 (MQ=255)                                                                       aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCg                                                                  >  3:246496/1‑149 (MQ=255)                                                                        gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 <  6:338282/149‑1 (MQ=255)                                                                        gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 <  1:14537/149‑1 (MQ=255)                                                                        gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGAGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  4:2784/1‑149 (MQ=255)                                                                               aaGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         <  6:249763/134‑1 (MQ=255)                                                                               aaGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         >  5:249763/1‑134 (MQ=255)                                                                                 gCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACt                                                        >  4:335282/1‑149 (MQ=255)                                                                                    aGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCt                                                                                                                  >  1:240196/1‑88 (MQ=255)                                                                                    aGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCt                                                                                                                  <  2:240196/88‑1 (MQ=255)                                                                                          tatGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATcgc                                               <  1:122208/149‑1 (MQ=255)                                                                                               tGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCg                                                                                     <  4:149232/106‑1 (MQ=255)                                                                                               tGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCg                                                                                     >  3:149232/1‑106 (MQ=255)                                                                                                   aCCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGtt                                                                                  >  4:116857/1‑105 (MQ=255)                                                                                                   aCCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGtt                                                                                  <  3:116857/105‑1 (MQ=255)                                                                                                    ccAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACgg                                     >  2:172421/1‑149 (MQ=255)                                                                                                      aaGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATACTTCAGTACCTGGTCGGTTAATACCTACGAAGTCGGGAACTGACTCAGGATCGCGCGGGCACgcg                                    >  8:312747/1‑146 (MQ=255)                                                                                                         cTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATAc                                                                             >  2:324583/1‑104 (MQ=255)                                                                                                         cTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATAc                                                                             <  1:324583/104‑1 (MQ=255)                                                                                                         cTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATc                                <  3:2784/149‑1 (MQ=255)                                                                                                            cAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCa                               <  7:34655/147‑1 (MQ=255)                                                                                                             aGCCCGCAGATGCATCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTAGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATAAGcc                            >  2:140955/1‑149 (MQ=255)                                                                                                              gCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGccc                           >  2:266485/1‑149 (MQ=255)                                                                                                               cccGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGcc                            <  4:128602/147‑1 (MQ=255)                                                                                                               cccGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGcc                            >  3:128602/1‑147 (MQ=255)                                                                                                                     gATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCg                                                                  >  5:312923/1‑103 (MQ=255)                                                                                                                     gATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCg                                                                  <  6:312923/103‑1 (MQ=255)                                                                                                                     gATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCtg                    <  3:196200/149‑1 (MQ=255)                                                                                                                      aTGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGt                                                                                   >  4:236539/1‑85 (MQ=255)                                                                                                                      aTGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGt                                                                                   <  3:236539/85‑1 (MQ=255)                                                                                                                         ccTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTgga                <  1:172421/149‑1 (MQ=255)                                                                                                                          cgaaGGCTCCGGCAGAGCCTGCGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggag               >  6:51179/4‑149 (MQ=255)                                                                                                                           gaaGGCTCCGGCAGAGCCTGCGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCtgt                   <  4:198333/142‑1 (MQ=255)                                                                                                                           gaaGGCTCCGGCAGAGCCTGCGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCtgt                   >  3:198333/3‑144 (MQ=255)                                                                                                                                 tCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACg                                      <  7:180030/119‑1 (MQ=255)                                                                                                                                 tCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACg                                      >  8:180030/1‑119 (MQ=255)                                                                                                                                   cGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGc      >  8:223269/1‑149 (MQ=255)                                                                                                                                       gcAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCtgtt  >  1:221917/1‑149 (MQ=255)                                                                                                                                       |                                                                                                                                                     TACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCTGTT  >  NC_002947/4379008‑4379290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑