Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 415,319 C→T 7.7% P29P (CCC→CCT)  waaC → ADP‑heptose‑‑LPS heptosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947415,3190CT 7.7% 150.3 / 2.2 52P29P (CCC→CCT) waaCADP‑heptose‑‑LPS heptosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base T (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCGCCCTGCACAGCCTCAGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTGGCGAGTGGAAGG  >  NC_002947/415172‑415461
                                                                                                                                                   |                                                                                                                                              
cgccgcCCTGCACAGCCTCTCCGGGCCGGACCTGATCGATACCGTGGCCGAGGGCGACTAAGTGCGGGTACTGATGATCAAGACCTCGTCGCTGGGGGATGTAATCGACATCATGCCGGCGCTTACCGATGCCGCCCATGCCATTccc                                                                                                                                                <  8:227947/148‑1 (MQ=255)
    gccCTGCACAGCCTCAGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCAt                                                                                                                                           <  3:239773/149‑1 (MQ=255)
     cccTGCACAGCCTCAGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATc                                                                                                                                          >  7:237937/1‑149 (MQ=255)
          cacaGCCTCAGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGttt                                                                                                                                     <  4:327310/149‑1 (MQ=255)
               ccTCAGAGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGAtt                                                                                                                                >  8:175751/1‑149 (MQ=255)
                   aGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTggg                                                                                                                             <  3:1472/148‑1 (MQ=255)
                      gggCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGggtggt                                                                                                                         <  7:55437/149‑1 (MQ=255)
                             aCCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGgaagaa                                                                                                                  <  7:175751/149‑1 (MQ=255)
                                  aTCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGc                                                                                                               <  8:106207/147‑1 (MQ=255)
                                      aTACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGcc                                                                                                         >  3:162132/1‑149 (MQ=255)
                                               ccGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATcccc                                                                                                <  2:206628/149‑1 (MQ=255)
                                                             gTGCGGGTACTGATCATCAAGACCTCGTAGCTGGGGGATGTAATCCACCCCCTGCCGGAGCTTACCGATGCCGCCCATGCCATTCCCGGCAGACGTTTCGCTTGGGTGGTGGAAGACGGCTTCGCCGAAATCCCCAGTTGGCACCCcgc                                                                                  >  6:240153/1‑149 (MQ=255)
                                                              tGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCcgcg                                                                                 >  6:191070/1‑149 (MQ=255)
                                                                cGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCgg                                                                                <  4:162132/148‑1 (MQ=255)
                                                                 gggTACTGAGCATCAAGCCCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCCCCCCGCGGTc                                                                              <  5:240153/149‑1 (MQ=255)
                                                                    tACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGAc                                                                           <  8:237937/149‑1 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCTGGCATCCGTTTCGATTGGGTGGTGGaagaag                                                                                                                 >  4:152888/1‑108 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCTGGCATCCGTTTCGATTGGGTGGTGGaagaag                                                                                                                 >  4:158090/1‑108 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCTGGCATCCGTTTCGATTGGGTGGTGGaagaag                                                                                                                 <  3:158090/108‑1 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCTGGCATCCGTTTCGATTGGGTGGTGGaagaag                                                                                                                 <  3:152888/108‑1 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGtt                                                                                                                                      >  6:13041/1‑87 (MQ=255)
                                                                       tGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGtt                                                                                                                                      <  5:13041/87‑1 (MQ=255)
                                                                        gATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGgtggtg                                                                                                                        >  7:261188/1‑100 (MQ=255)
                                                                        gATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGgtggtg                                                                                                                        <  8:261188/100‑1 (MQ=255)
                                                                        gATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGt                                                                               >  5:269052/1‑141 (MQ=255)
                                                                        gATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGt                                                                               <  6:269052/141‑1 (MQ=255)
                                                                         atcatcAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGt                                                                      >  4:221257/1‑149 (MQ=255)
                                                                         atcatcAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGt                                                                      >  3:97075/1‑149 (MQ=255)
                                                                          tcatcaAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTc                                                                     <  3:221257/149‑1 (MQ=255)
                                                                            atcaAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCAt                                                                   >  6:307958/1‑149 (MQ=255)
                                                                             tcaAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATc                                                                  >  7:120917/1‑149 (MQ=255)
                                                                              caAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATcc                                                                 >  4:345430/1‑149 (MQ=255)
                                                                                    cTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTgg                                                           >  3:262414/1‑149 (MQ=255)
                                                                                     tcgtcgCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGc                                                                                                          <  8:241887/101‑1 (MQ=255)
                                                                                     tcgtcgCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGc                                                                                                          >  7:241887/1‑101 (MQ=255)
                                                                                        tcgCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGAt                                                       <  3:345430/149‑1 (MQ=255)
                                                                                              ggggATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCg                                                 <  8:120917/149‑1 (MQ=255)
                                                                                                   tGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGgc                                            <  4:97075/149‑1 (MQ=255)
                                                                                                          cacaCCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGaa                                     <  7:205427/149‑1 (MQ=255)
                                                                                                           acacCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGgcg                                           >  7:328051/1‑142 (MQ=255)
                                                                                                           acacCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGgcg                                           <  8:328051/142‑1 (MQ=255)
                                                                                                            cacCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAAcc                                   >  4:99207/1‑149 (MQ=255)
                                                                                                                    cGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCa                            >  2:257485/1‑148 (MQ=255)
                                                                                                                          ttACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCaa                                        >  7:316086/1‑130 (MQ=255)
                                                                                                                          ttACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCaa                                        <  8:316086/130‑1 (MQ=255)
                                                                                                                                 tGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTg              <  4:262414/149‑1 (MQ=255)
                                                                                                                                 tGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTg              >  2:44298/1‑149 (MQ=255)
                                                                                                                                            ccATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTGGCGAGTGGaa    <  8:27162/148‑1 (MQ=255)
                                                                                                                                             cATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCaaa                          >  6:283700/1‑125 (MQ=255)
                                                                                                                                             cATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCaaa                          <  5:283700/125‑1 (MQ=255)
                                                                                                                                             cATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAaga                 >  7:265048/1‑134 (MQ=255)
                                                                                                                                             cATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAaga                 <  8:265048/134‑1 (MQ=255)
                                                                                                                                             cATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTGGCGAGTGGAAgg  >  4:211081/1‑149 (MQ=255)
                                                                                                                                                   cGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATc                                                      >  7:16564/1‑91 (MQ=255)
                                                                                                                                                   cGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATc                                                      <  8:16564/91‑1 (MQ=255)
                                                                                                                                                   cGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATc                     <  6:63661/124‑1 (MQ=255)
                                                                                                                                                   cGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATc                     >  5:63661/1‑124 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CGCCGCCCTGCACAGCCTCAGCGGGCCGGACCTGATCGATACCGTGGCCGAGGTCGACTAAGTGCGGGTACTGATCATCAAGACCTCGTCGCTGGGGGATGTAATCCACACCCTGCCGGCGCTTACCGATGCCGCCCATGCCATTCCCGGCATCCGTTTCGATTGGGTGGTGGAAGAAGGCTTCGCCGAAATCCCCAGTTGGCACCCCGCGGTCGACCAGGTCATCCCGGTGGCGATCCGTCGCTGGCGCAAGAACCTCTGGCAAACCATCAAGAGTGGCGAGTGGAAGG  >  NC_002947/415172‑415461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑