Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,419,577 T→G 6.2% I115S (ATC→AGC)  PP_3910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,419,5770TG 6.2% 192.2 / 2.5 62I115S (ATC→AGC) PP_3910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base G (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGACGGCCTGATTGA  >  NC_002947/4419429‑4419721
                                                                                                                                                    |                                                                                                                                                  
cACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTAt                                                                                                                                                    <  7:98971/149‑1 (MQ=255)
      tGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCg                                                                                                                                             <  8:238789/149‑1 (MQ=255)
      tGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCg                                                                                                                                             <  8:311969/149‑1 (MQ=255)
          gtgcTGACGACTACGGCTACCGCTCGCTGCGCCGGGCGGCCTAGCATGCCAAGGGCACAGACTAAGAGGGGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGTCGATAGCGT‑CCgggca                                                                                                                                         <  1:69714/146‑3 (MQ=255)
           gCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGAc                                                                                                                                         >  3:121318/1‑148 (MQ=255)
              tGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGaaa                                                                                                                                     >  7:324028/1‑149 (MQ=255)
                   aaTACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTgg                                                                                                                                <  1:116995/149‑1 (MQ=255)
                   aaTACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTgg                                                                                                                                <  7:309885/149‑1 (MQ=255)
                           tACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCAGAGAAGGCTGAGCACCAGCGCCGGGACGACATCTGGTGAGCACACTGCCCGGACGCTAGCGA‑CCTGGACGAAATGTGGGAGG‑CCAt                                                                                                                       >  5:294740/1‑149 (MQ=255)
                                ctcgctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCg                                                                                                                  >  7:326466/1‑149 (MQ=255)
                                           gggCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTggc                                                                                                       >  4:23799/1‑149 (MQ=255)
                                           gggCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTggc                                                                                                       <  7:215714/149‑1 (MQ=255)
                                            ggcggcCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAg                                                                                                                             >  8:98873/1‑127 (MQ=255)
                                            ggcggcCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAg                                                                                                                             <  7:98873/127‑1 (MQ=255)
                                             gcggcCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTggcgg                                                                                                     >  6:145134/1‑149 (MQ=255)
                                                gcCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCAt                                                                                                  >  1:316827/1‑149 (MQ=255)
                                                 ccAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATc                                                                                                 >  7:188476/1‑149 (MQ=255)
                                                   aaGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTg                                                                                                         <  1:232866/140‑1 (MQ=255)
                                                   aaGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTg                                                                                                         >  2:232866/1‑139 (MQ=255)
                                                        tGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCa                                                                                          <  5:145134/149‑1 (MQ=255)
                                                          ccAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCGCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGt                                                                                        >  5:169568/1‑149 (MQ=255)
                                                           cAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGCCCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTg                                                                                       >  3:65546/1‑149 (MQ=255)
                                                              gggCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCg                                                                                    <  4:121318/149‑1 (MQ=255)
                                                                        aaaGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGc                                                                            >  5:50669/1‑147 (MQ=255)
                                                                        aaaGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGc                                                                            <  6:50669/147‑1 (MQ=255)
                                                                           gagGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAg                                                                       >  8:126067/1‑149 (MQ=255)
                                                                                 cGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACg                                                                  <  8:326466/148‑1 (MQ=255)
                                                                                        ccGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATAcc                                                          >  1:23176/1‑149 (MQ=255)
                                                                                          gagaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGa                                                              >  2:42200/1‑143 (MQ=255)
                                                                                          gagaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGa                                                              <  1:42200/143‑1 (MQ=255)
                                                                                           agaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGta                                                       >  2:147096/1‑149 (MQ=255)
                                                                                            gaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGtat                                                      <  8:188476/149‑1 (MQ=255)
                                                                                                  tGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGa                                                <  6:169568/149‑1 (MQ=255)
                                                                                                    aGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAAc                                              >  5:186066/1‑149 (MQ=255)
                                                                                                       aCCAGCGCCTGGACGACCTGTGGATCGCACACTGCGCGGACGCTATCGA‑CCGGTACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGTCGGCATCTGGCTGAGTGGCGTTATGTGCTTCAGCCACGGCTATACCTTATACCTGAACCTg                                           <  6:294740/149‑1 (MQ=255)
                                                                                                       aCCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTg                                           >  4:189172/1‑149 (MQ=255)
                                                                                                             gcCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGgcagc                                     <  2:23176/149‑1 (MQ=255)
                                                                                                                  gacgacCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATAc                                                    <  1:138596/129‑1 (MQ=255)
                                                                                                                  gacgacCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAg                                  <  6:186066/147‑1 (MQ=255)
                                                                                                                  gacgacCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGc                                >  8:247183/1‑149 (MQ=255)
                                                                                                                    cgacCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCg                              <  7:126067/149‑1 (MQ=255)
                                                                                                                       ccTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCt                            >  5:114302/1‑148 (MQ=255)
                                                                                                                        ctctGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTggg                                         >  1:291489/1‑134 (MQ=255)
                                                                                                                        ctctGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTggg                                         <  2:291489/134‑1 (MQ=255)
                                                                                                                         tctGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGAc                         >  8:330046/1‑149 (MQ=255)
                                                                                                                             gAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACg                        <  7:247183/146‑1 (MQ=255)
                                                                                                                                  cacaCTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTc                <  3:189172/149‑1 (MQ=255)
                                                                                                                                  cacaCTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGA‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCaa                                   <  7:90711/130‑1 (MQ=255)
                                                                                                                                  cacaCTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGA‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCaa                                   >  8:90711/1‑130 (MQ=255)
                                                                                                                                    cacTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCAGCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGa              <  3:23799/149‑1 (MQ=255)
                                                                                                                                     acTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGt                                                                                  >  8:133494/1‑80 (MQ=255)
                                                                                                                                     acTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGt                                                                                  <  7:133494/80‑1 (MQ=255)
                                                                                                                                     acTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGTGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGAc             <  2:316827/149‑1 (MQ=255)
                                                                                                                                        tcccGGTCGATAGCGT‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTggg                                         >  5:88541/2‑118 (MQ=255)
                                                                                                                                        tcccGGTCGATAGCGT‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTggg                                         <  6:88541/117‑1 (MQ=255)
                                                                                                                                             gACGCTATCGA‑CCGGGACGAAATGTGGGAGGTCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCt                            >  1:37611/1‑127 (MQ=255)
                                                                                                                                             gACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCt                            <  2:37611/126‑1 (MQ=255)
                                                                                                                                                gCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGtat                                                      >  5:358112/1‑97 (MQ=255)
                                                                                                                                                gCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGtat                                                      <  6:358112/97‑1 (MQ=255)
                                                                                                                                                gCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGACGGTCTGATTga  <  4:65546/149‑1 (MQ=255)
                                                                                                                                                  tATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCt                            >  3:76738/1‑121 (MQ=255)
                                                                                                                                                  tATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCt                            <  4:76738/121‑1 (MQ=255)
                                                                                                                                                  tATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGACGGCCTGAtt    >  6:289150/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGA‑CCGGGACGAAATGTGGGAGG‑CCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTATACCTGAACCTGGGCAGCAAGGCCGCTGACGGCGATGTCGACGGCCTGATTGA  >  NC_002947/4419429‑4419721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑