Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,459,754 T→A 9.8% L260Q (CTG→CAG)  trkH‑I → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,459,7540TA 9.8% 138.5 / 5.5 51L260Q (CTG→CAG) trkH‑Ipotassium transporter
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base A (3/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

ATCAATCATTCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATCGTTGCG  >  NC_002947/4459606‑4459896
                                                                                                                                                    |                                                                                                                                               
atcaatcaTTCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCt                                                                                                                                                 >  4:172222/1‑149 (MQ=255)
    atcaTTCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGTGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGt                                                                                                                                             <  5:87462/149‑1 (MQ=255)
        ttCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGc                                                                                                                                          >  3:330545/1‑148 (MQ=255)
        ttCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCAGCGTGGc                                                                                                                                          >  5:263309/1‑148 (MQ=255)
        ttCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCAGCGTGGc                                                                                                                                          >  8:65041/1‑148 (MQ=255)
           gATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCAGCGTGGcgac                                                                                                                                       <  6:263309/148‑4 (MQ=255)
                         cAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCAGCGTGGcgac                                                                                                                                       >  4:55949/1‑131 (MQ=255)
                         cAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCAGCGTGGcgac                                                                                                                                       <  3:55949/134‑4 (MQ=255)
                             cGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGc                                                                                                                                 >  2:98001/1‑136 (MQ=255)
                             cGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGc                                                                                                                                 <  1:98001/136‑1 (MQ=255)
                             cGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAg                                                                                                                     >  4:104793/1‑148 (MQ=255)
                                    ttCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCACCACGCTGCGTGGCCAGCGAGGCCTCCTGCTCAAGGCCCATc                                                                                                              >  6:221367/1‑148 (MQ=255)
                                         tACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATc                                                                                                                                      >  2:33719/1‑119 (MQ=255)
                                         tACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATc                                                                                                                                      <  1:33719/119‑1 (MQ=255)
                                            cTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑cgcg                                                                                                    >  8:275854/1‑149 (MQ=255)
                                                     cTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAg                                                                                                                                   <  4:346070/110‑1 (MQ=255)
                                                     cTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAg                                                                                                                                   >  3:346070/1‑110 (MQ=255)
                                                                        cagccagcGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTg                                                                        <  4:330545/149‑1 (MQ=255)
                                                                             agcGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCAt                                                                                                                                       >  5:174319/1‑82 (MQ=255)
                                                                             agcGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCAt                                                                                                                                       <  6:174319/82‑1 (MQ=255)
                                                                                                 tgGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCTATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTctgc                                               <  3:172222/149‑1 (MQ=255)
                                                                                                    tcatcaTGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTctgcctg                                            >  7:16394/1‑149 (MQ=255)
                                                                                                       tcatGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGca                                          >  1:146885/1‑148 (MQ=255)
                                                                                                        catGATACTCGGCAGCCTCCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTACATTGGTTATCTCGTCATCGTCTGGCTCACCGTCGGAACCTGGCTctgcctgga                                          >  2:85584/1‑145 (MQ=255)
                                                                                                         atGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAg                                       >  2:168548/1‑149 (MQ=255)
                                                                                                           gATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGc                                      >  6:183928/1‑148 (MQ=255)
                                                                                                           gATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGc                                      >  1:196470/1‑148 (MQ=255)
                                                                                                             tACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAAt                                   <  8:16394/149‑1 (MQ=255)
                                                                                                               cTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTc                                                                    >  8:257894/1‑114 (MQ=255)
                                                                                                               cTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTc                                                                    <  7:257894/114‑1 (MQ=255)
                                                                                                               cTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCt                                 <  1:168548/149‑1 (MQ=255)
                                                                                                                tCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTg                                >  2:43896/1‑149 (MQ=255)
                                                                                                                tCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTATGCCTGCACAGCAATCTg                                >  8:284613/1‑149 (MQ=255)
                                                                                                                  ggCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGTTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGc                              <  2:146885/149‑1 (MQ=255)
                                                                                                                  ggCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTg                                <  5:183928/147‑1 (MQ=255)
                                                                                                                        cTGCCCTTCACATTGTATGTCACCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCtggtg                        <  5:221367/149‑1 (MQ=255)
                                                                                                                            ccTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGAt                    <  7:284613/149‑1 (MQ=255)
                                                                                                                              ttCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTTCCGCGGTTTCATTGGTTTTCTCGTCTTCGTCTg                                                                        <  1:36479/96‑1 (MQ=255)
                                                                                                                              ttCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTg                                                                        >  2:36479/1‑95 (MQ=255)
                                                                                                                                   aTTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTc             <  7:275854/149‑1 (MQ=255)
                                                                                                                                      gTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGc                              <  6:358934/129‑1 (MQ=255)
                                                                                                                                      gTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGc                              >  5:358934/1‑129 (MQ=255)
                                                                                                                                      gTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGt           <  3:104793/148‑1 (MQ=255)
                                                                                                                                       tATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTAt         >  1:57650/1‑149 (MQ=255)
                                                                                                                                        aTGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATc        >  5:297949/1‑149 (MQ=255)
                                                                                                                                         tGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATc        <  2:57650/148‑1 (MQ=255)
                                                                                                                                          gTCGCCACGCTGCGTGGCCATCGAGGCAACCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGACCCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTGCGTATCGt      >  5:229461/1‑149 (MQ=255)
                                                                                                                                             gCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATCGTTg    <  1:43896/148‑1 (MQ=255)
                                                                                                                                             gCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATCGTTGc   >  5:135720/1‑149 (MQ=255)
                                                                                                                                              ccACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTGATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACCGCGATCTGGGCTGGTGGGATGCGTTTGGTCTGGTTGCg  >  4:212803/1‑149 (MQ=255)
                                                                                                                                              ccACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTctgcct                                             >  8:87597/1‑106 (MQ=255)
                                                                                                                                              ccACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATCGTTGCg  <  6:135720/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ATCAATCATTCGATGTCGCTGGTTTCAACCGGTGGTTTCTCTACCTCGGATGCCTCGCTGGGCCATTGGGAGCAGCCAGCGGTGCACTGGGTGGCTGTGGTCATCATGATACTCGGCAGCCTGCCCTTCACATTGTATGTCGCCACGCTGCGTGGCCATCGAGGCATCCTGCTCAAGGACCATCAAGTT‑CGCGGTTTCATTGGTTTTCTCGTCTTCGTCTGGCTCACCGTCGGAACCTGGCTCTGCCTGCACAGCAATCTGGCCTGGTGGGATGCGTTTCGTATCGTTGCG  >  NC_002947/4459606‑4459896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑