Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,479,901 A→T 7.5% V12V (GTA→GTT) ‡ PP_3971 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,479,9010AT 7.5% 155.2 / 3.5 53V12V (GTA→GTT) ‡PP_3971hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/23);  new base T (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCTGGCCCGCGAG  >  NC_002947/4479755‑4480049
                                                                                                                                                  |                                                                                                                                                    
tGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGc                                                                                                                                                    <  1:236211/149‑1 (MQ=255)
  ggCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCgg                                                                                                                                                  <  6:235294/149‑1 (MQ=255)
                 gATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTg                                                                                                                                       <  5:118540/145‑1 (MQ=255)
                 gATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTg                                                                                                                                       >  6:118540/1‑145 (MQ=255)
                      aaaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGAc                                                                                                                              >  7:214271/1‑149 (MQ=255)
                        aGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGCTACGGCAAtccagaa                                                                                                                                        <  5:109392/137‑7 (MQ=255)
                        aGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGCTACGGCAAtccagaa                                                                                                                                        >  6:109392/1‑131 (MQ=255)
                          cAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCAt                                                                                                                                  >  8:5604/1‑141 (MQ=255)
                          cAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCAt                                                                                                                                  <  7:5604/141‑1 (MQ=255)
                           aGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCtgtg                                                                                                                         <  5:159260/149‑1 (MQ=255)
                                   tGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGc                                                                                                                                                    <  6:85708/114‑1 (MQ=255)
                                   tGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGc                                                                                                                                                    >  5:85708/1‑114 (MQ=255)
                                     ccGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATGATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGAGGCAATTCTTCTGCTCATCGACCGGTGGGCCATCGt                                                                                                                >  5:77327/1‑148 (MQ=255)
                                        cAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGc                                                                                                            <  5:245094/149‑1 (MQ=255)
                                        cAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGc                                                                                                            <  8:110601/149‑1 (MQ=255)
                                             tCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGtt                                                                                                       >  2:329857/1‑149 (MQ=255)
                                               aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTcc                                                                                                     >  4:237512/1‑149 (MQ=255)
                                                   aggTGCCGGGTGCAGCACCTGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCg                                                                                                    >  6:335954/3‑146 (MQ=255)
                                                   aggTGCCGGGTGCAGCACCTGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGa                                                                                                  <  5:335954/146‑1 (MQ=255)
                                                        ccAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTgg                                                                                                                        >  4:137613/1‑121 (MQ=255)
                                                        ccAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTgg                                                                                                                        <  3:137613/121‑1 (MQ=255)
                                                         cAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCg                                                                                                                 <  4:104852/127‑1 (MQ=255)
                                                         cAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCg                                                                                                                 >  3:104852/1‑127 (MQ=255)
                                                             tgctgcACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGAcc                                                                                       <  8:214271/149‑1 (MQ=255)
                                                                    cccGGCACCTTGGGCCCTGACCACCTCTACAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCg                                                                                >  8:211881/1‑149 (MQ=255)
                                                                              tGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                                                                                                                  >  8:62485/1‑105 (MQ=255)
                                                                              tGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATc                                                                                                                  <  7:62485/105‑1 (MQ=255)
                                                                                  cccTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGc                                                                      <  5:216780/145‑1 (MQ=255)
                                                                                  cccTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGc                                                                      >  6:216780/1‑145 (MQ=255)
                                                                                              ctAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGcc                                                                                 >  4:201396/1‑122 (MQ=255)
                                                                                              ctAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGcc                                                                                 <  3:201396/122‑1 (MQ=255)
                                                                                                      ggaATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGccc                                               >  7:22022/1‑148 (MQ=255)
                                                                                                                tGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCttgtt                                    <  3:237512/149‑1 (MQ=255)
                                                                                                                     ggTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCt                               >  4:327489/1‑149 (MQ=255)
                                                                                                                     ggTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCt                               >  6:4319/1‑149 (MQ=255)
                                                                                                                           gCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGa                          >  5:5460/1‑148 (MQ=255)
                                                                                                                            ccAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGata                        >  2:179696/1‑149 (MQ=255)
                                                                                                                            ccAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTATGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGata                        >  7:85225/1‑149 (MQ=255)
                                                                                                                                    ttCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCttgtt                                    <  3:3403/129‑1 (MQ=255)
                                                                                                                                    ttCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCttgtt                                    >  4:3403/1‑129 (MQ=255)
                                                                                                                                    ttCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGt                >  3:184976/1‑149 (MQ=255)
                                                                                                                                     tCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTgg                             >  7:164365/1‑135 (MQ=255)
                                                                                                                                     tCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTgg                             <  8:164365/135‑1 (MQ=255)
                                                                                                                                     gaagaaTTGCCGCTACGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGc                                                 <  4:94055/110‑1 (MQ=255)
                                                                                                                                     gaagaaTTGCCGCTACGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGc                                                 >  3:94055/6‑115 (MQ=255)
                                                                                                                                         gagtGCCGTATCTGCAATTCTTCTGCTCCTCGACCTGTGGGCCATCGTCAGCGTGGTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCt            <  6:5460/145‑1 (MQ=255)
                                                                                                                                         gATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCTg           <  4:184976/149‑1 (MQ=255)
                                                                                                                                              ccGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGCTGCCGTGAAAGCGGCCTGGGCTCTCCTGATAGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCTGGCCcg      >  7:178643/1‑149 (MQ=255)
                                                                                                                                              ccGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCt                               >  5:181064/1‑124 (MQ=255)
                                                                                                                                              ccGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCt                               <  6:181064/124‑1 (MQ=255)
                                                                                                                                               cGTAGCGGCATTGCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGAAATGACCGATGCCGTTGTAGGGGCCTGGGCTCTCCTGATGGTGGCCCTACCGCTTGTTGGGCTGGGGATTTGGGGCGTTg              <  3:130427/140‑1 (MQ=255)
                                                                                                                                               cGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTggg                            >  7:179670/1‑126 (MQ=255)
                                                                                                                                               cGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTggg                            <  8:179670/126‑1 (MQ=255)
                                                                                                                                                  aGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCTGGCCCGCGAg  >  4:151660/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGACAAGACCGATGCCGTGAAAGCGGCCTGGGCTCTCCTGATCGTGGCCCTACCGCTTGTTGGCCTGGGGATATGGGGCGTTGCTGGCCCGCGAG  >  NC_002947/4479755‑4480049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑