Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,513,847 G→C 7.0% S382W (TCG→TGG) ‡ ftsK ← DNA translocase FtsK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,513,8470GC 7.0% 176.2 / 3.5 57S382W (TCG→TGG) ‡ftsKDNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TCTTGCCAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAGGGCAAGGTGCCTTCCACGG  >  NC_002947/4513701‑4513991
                                                                                                                                                  |                                                                                                                                                
tCTTGCCAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCg                                                                                                                                                  >  4:284046/1‑147 (MQ=255)
tCTTGCCAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCg                                                                                                                                                  <  3:284046/147‑1 (MQ=255)
     ccAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATTCGATCCAc                                                                                                                                           >  6:342502/1‑149 (MQ=255)
     ccAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATcc                                                                                                                                             >  7:209696/1‑147 (MQ=255)
     ccAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATcc                                                                                                                                             <  8:209696/147‑1 (MQ=255)
        gggATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATTCGATCCAcccc                                                                                                                                        <  5:342502/149‑3 (MQ=255)
           aTGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCAc                                                                                                                                     <  2:113377/149‑1 (MQ=255)
                cTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTcaa                                                                                                                                >  7:216654/1‑147 (MQ=255)
                cTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCca                                                                                                                                <  1:152137/149‑1 (MQ=255)
                 tCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCca                                                                                                                                >  5:255991/1‑148 (MQ=255)
                         tCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGACCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACt                                                                                                                       >  7:162729/1‑149 (MQ=255)
                           gCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCGGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTcc                                                                                                                     >  2:285325/1‑149 (MQ=255)
                               acTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGa                                                                                                                 <  7:165344/149‑1 (MQ=255)
                               acTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGACCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGa                                                                                                                 <  8:162729/149‑1 (MQ=255)
                                 tGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGc                                                                                                               >  7:34503/1‑149 (MQ=255)
                                    tCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCg                                                                                                                                         <  7:39947/120‑1 (MQ=255)
                                    tCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCg                                                                                                                                         >  8:39947/1‑120 (MQ=255)
                                             gCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCcag                                                                                                    >  1:65716/1‑148 (MQ=255)
                                               gAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCcagcag                                                                                                 >  4:263764/1‑149 (MQ=255)
                                                   gcgcCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTGCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTgg                                                                                             >  6:215797/1‑149 (MQ=255)
                                                        aGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGAc                                                                                        >  5:234162/1‑149 (MQ=255)
                                                                 gCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCaa                                                                               <  6:255991/149‑1 (MQ=255)
                                                                  ccAGGTTGGCGATGCGGCTGACCTTCACGCCGGCGGCCGGCTGGATTTCGTAAGGGGTAATGACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAg                                                                              <  1:285325/149‑1 (MQ=255)
                                                                  ccAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATc                                                                                                                                              >  4:30304/1‑85 (MQ=255)
                                                                  ccAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATc                                                                                                                                              <  3:30304/85‑1 (MQ=255)
                                                                    aGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGa                                                                            >  1:189842/1‑149 (MQ=255)
                                                                           cGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGc                                                                     <  8:139694/149‑1 (MQ=255)
                                                                           cGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGc                                                                     <  8:34503/149‑1 (MQ=255)
                                                                           cGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGc                                                                     <  8:144404/149‑1 (MQ=255)
                                                                           cGAGGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGc                                                                     <  4:69947/149‑1 (MQ=255)
                                                                             aTGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGa                                                                   <  3:263764/149‑1 (MQ=255)
                                                                              tGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGaa                                                                  <  2:65716/149‑1 (MQ=255)
                                                                                 ggCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATAc                                                               >  3:5480/1‑149 (MQ=255)
                                                                                        cTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTgg                                                                                             >  2:333609/1‑112 (MQ=255)
                                                                                        cTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTgg                                                                                             <  1:333609/112‑1 (MQ=255)
                                                                                         ttCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCAc                                                                                                                                           >  2:2083/1‑65 (MQ=255)
                                                                                         ttCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAtct                                                        >  1:180219/1‑148 (MQ=255)
                                                                                           cACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAtcttc                                                      <  3:252630/148‑1 (MQ=255)
                                                                                           cACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAtct                                                        <  2:180219/146‑1 (MQ=255)
                                                                                              gCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAtcttcttct                                                  >  1:200603/1‑149 (MQ=255)
                                                                                              gCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAtcttcttct                                                  >  3:147220/1‑149 (MQ=255)
                                                                                                cAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTg                                                                       >  2:192963/1‑126 (MQ=255)
                                                                                                cAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTg                                                                       <  1:192963/126‑1 (MQ=255)
                                                                                                      ccggcTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGcc                                          <  4:123197/149‑1 (MQ=255)
                                                                                                        ggcTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCtt                                                                                                                   >  4:13648/1‑74 (MQ=255)
                                                                                                        ggcTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCtt                                                                                                                   <  3:13648/74‑1 (MQ=255)
                                                                                                         gcTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCggg                                       >  3:30450/1‑149 (MQ=255)
                                                                                                           tGGAGTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTc                                     <  6:234162/149‑1 (MQ=255)
                                                                                                             gATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGa                                   <  2:200603/149‑1 (MQ=255)
                                                                                                                        gggTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATc                        >  4:123770/1‑149 (MQ=255)
                                                                                                                         ggTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGAGCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATc                        >  6:82678/1‑148 (MQ=255)
                                                                                                                           tAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAg                     <  4:30450/149‑1 (MQ=255)
                                                                                                                             aTCCCCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAgg                    <  5:82678/148‑1 (MQ=255)
                                                                                                                                   gggccgggGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAGGGCAAGGt             <  3:123770/149‑1 (MQ=255)
                                                                                                                                         cggTGGATTCGATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGa                                   >  2:155605/2‑121 (MQ=255)
                                                                                                                                         cggTGGATTCGATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGa                                   <  1:155605/120‑1 (MQ=255)
                                                                                                                                              gATCGAATCCACCTCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAGGGCAAGGTGCCTTCCACgg  <  4:5480/149‑1 (MQ=255)
                                                                                                                                               aTCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCgg                                        >  7:266471/1‑110 (MQ=255)
                                                                                                                                               aTCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCgg                                        <  8:266471/110‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCTTGCCAGGGATGACCTCGACCACTCGCACACTGGTCACGGCCAGCGAACGCGCCAGGTCCTTGGCCAGGTTGGCGATGCGGCTGACCTTCACGCCAGCGGCCGGCTGGATTTCGTAACGGGTAATCACCGGGCCGGGGTGGATCGAATCCACCGCCACTTCCACACCGAACTCCTTGAGCTTGATTTCCAGCAGCTGGCCGACGCCGGCCAAGGATTCTGGCGAATACTCGATCTTCTTCTGTTCGGCCGGGTCGAGGATGGAAATCGAGGGCAAGGTGCCTTCCACGG  >  NC_002947/4513701‑4513991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑