Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,516,192	C→G	5.8%	Y19* (TAC→TAG)	ate →	arginyl‑tRNA‑‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,516,192	0	C	G	5.8%	221.3 / 3.1	69	Y19* (TAC→TAG)	ate	arginyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base G (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

CTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGC  >  NC_002947/4516044‑4516332                                                                                                                                                      |                                                                                                                                              cTTGACCAGCCCAATAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                               <  7:328933/149‑1 (MQ=255) cTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                               >  7:307341/1‑149 (MQ=255)  ttGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAcc                                                                                                                                              >  7:237010/1‑149 (MQ=255)     aCCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTg                                                                                                                                            <  1:77058/148‑1 (MQ=255)          cccAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                      >  8:51151/1‑149 (MQ=255)          cccAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                      >  2:86635/1‑149 (MQ=255)            cAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                    <  1:344982/149‑1 (MQ=255)            cAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                      >  2:344982/1‑147 (MQ=255)            cAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCGGCAGGTAGCTGCAggagtg                                                                                                                                    <  3:20525/149‑3 (MQ=255)             aaCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGgaggag                                                                                                                                   >  3:278238/1‑149 (MQ=255)              aCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGgaggag                                                                                                                                   >  3:15733/1‑148 (MQ=255)                 gcgcTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                      >  6:268275/1‑142 (MQ=255)                 gcgcTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                      <  5:268275/142‑1 (MQ=255)                     ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCGGCAGGTAGCTGCAggagtg                                                                                                                                    <  2:12707/140‑3 (MQ=255)                     ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCGGCAGGTAGCTGCAggagtg                                                                                                                                    >  1:12707/1‑138 (MQ=255)                             ttGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGt                                                                                                                  <  5:43610/149‑1 (MQ=255)                                ccTAGGCTAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGACGGAGGAGCAGGCG‑ACCACGCTGTTcc                                                                                                               >  5:146531/1‑149 (MQ=255)                                ccTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGATTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGtt                                                                                                                 >  1:95936/1‑148 (MQ=255)                                    ggCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGa                                                                                                           <  6:303766/149‑1 (MQ=255)                                    cgCGAGTTCACGTAGCTGGCTTAGACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGGTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTc                                                                                                             <  4:33160/146‑1 (MQ=255)                                          ttCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCccag                                                                                                     <  8:237010/149‑1 (MQ=255)                                           tCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGAccc                                                                                                        >  3:113616/1‑145 (MQ=255)                                           tCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGAccc                                                                                                        <  4:113616/145‑1 (MQ=255)                                             ccGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCccagcca                                                                                                  <  8:307341/149‑1 (MQ=255)                                                  gCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGa                                                                                             >  6:334840/1‑149 (MQ=255)                                                      gCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATg                                                                                           <  1:86635/147‑1 (MQ=255)                                                      gCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATg                                                                                           <  7:51151/147‑1 (MQ=255)                                                       cTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGAc                                                                                        >  3:84388/1‑149 (MQ=255)                                                       cTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGAc                                                                                        >  8:138114/1‑149 (MQ=255)                                                       cTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGAc                                                                                        >  2:99874/1‑149 (MQ=255)                                                         tACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGt                                                                                      >  3:260682/1‑149 (MQ=255)                                                             cTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCAc                                                                                  >  5:285948/1‑149 (MQ=255)                                                                aaaaaaaaGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACgt                                                                                <  6:285948/148‑1 (MQ=255)                                                                aaaaaaaaGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACgt                                                                                <  4:15733/148‑1 (MQ=255)                                                                 aaaaaaaGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACgtgt                                                                              <  2:95936/149‑1 (MQ=255)                                                                       aGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGAccc                                                                                                        <  6:167396/117‑1 (MQ=255)                                                                             aCCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGt                                                                  <  6:146531/149‑1 (MQ=255)                                                                             aCCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGt                                                                  <  4:260682/149‑1 (MQ=255)                                                                               cGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                <  7:138114/149‑1 (MQ=255)                                                                               cGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                >  7:118125/1‑149 (MQ=255)                                                                                gAATGGG‑GTGATCATGTAAGAGTTGGCGGGGGTTAAGTTCTATTCCACTCATGCCCACTCCTGCATCTACCTGGCGGTGGTTCATGCGTACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                <  1:99874/149‑1 (MQ=255)                                                                                gAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGc                                                                                                                              <  4:277195/87‑1 (MQ=255)                                                                                gAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGc                                                                                                                              >  3:277195/1‑87 (MQ=255)                                                                                     gg‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATg                                                          <  4:278238/149‑1 (MQ=255)                                                                                      g‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGTCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                         >  8:126579/1‑149 (MQ=255)                                                                                           aTCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                 <  4:20258/137‑1 (MQ=255)                                                                                           aTCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                 >  3:20258/1‑137 (MQ=255)                                                                                                gACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCCAGCTGTTCCTCGACCACAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTagcc                                                >  6:72145/1‑149 (MQ=255)                                                                                                gACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgcc                                                >  4:113549/1‑149 (MQ=255)                                                                                                gACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgcc                                                >  4:108715/1‑149 (MQ=255)                                                                                                gACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgc                                                 >  5:292934/1‑148 (MQ=255)                                                                                                gACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCAGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgc                                                 <  6:292934/148‑1 (MQ=255)                                                                                                  cAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                <  5:251874/131‑1 (MQ=255)                                                                                                  cAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                >  6:251874/1‑131 (MQ=255)                                                                                                       ttGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTgg                                         <  8:118125/149‑1 (MQ=255)                                                                                                                   aaGTTCTATGCCACTCAGTCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTa                             <  7:126579/149‑1 (MQ=255)                                                                                                                      ttCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTgg                                         >  1:298373/1‑134 (MQ=255)                                                                                                                      ttCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTgg                                         <  2:298373/134‑1 (MQ=255)                                                                                                                      ttCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGacca                                   <  1:166036/140‑1 (MQ=255)                                                                                                                      ttCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGacca                                   >  2:166036/1‑140 (MQ=255)                                                                                                                        cTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCAAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGAGACCACCTGTACCGcc                        >  8:31528/1‑149 (MQ=255)                                                                                                                             cctcTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGAt                                                                                            <  6:201880/73‑1 (MQ=255)                                                                                                                             cctcTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGAt                                                                                            >  5:201880/4‑76 (MQ=255)                                                                                                                             ccACTCAGCCCCACTCCTGCAGCTACCTGCCGGATGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATCGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCacac                   <  3:108715/149‑1 (MQ=255)                                                                                                                             ccACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCacac                   <  3:113549/149‑1 (MQ=255)                                                                                                                                       ccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCg                                       <  4:246016/119‑1 (MQ=255)                                                                                                                                       ccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCg                                       >  3:246016/1‑119 (MQ=255)                                                                                                                                       ccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                 >  6:117919/1‑141 (MQ=255)                                                                                                                                       ccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                 <  5:117919/141‑1 (MQ=255)                                                                                                                                        cACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTg        >  2:120552/1‑149 (MQ=255)                                                                                                                                          cTCCGGCAGGTAGCTGCAGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGc       >  5:244534/1‑148 (MQ=255)                                                                                                                                              tGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGc  >  8:124081/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                              CTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGG‑TTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCG‑ACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGC  >  NC_002947/4516044‑4516332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑