Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,519,451 C→T 9.4% E23E (GAG→GAA)  clpA ← ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,519,4510CT 9.4% 176.1 / 7.3 64E23E (GAG→GAA) clpAATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base T (3/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GACAGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTCACAGAGTAGCGGATGCTG  >  NC_002947/4519307‑4519588
                                                                                                                                                |                                                                                                                                         
gACAGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCa                                                                                                                                        <  6:344826/148‑1 (MQ=255)
 aCAGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCAt                                                                                                                                       >  3:1727/1‑148 (MQ=255)
 aCAGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATg                                                                                                                                      <  4:1727/149‑1 (MQ=255)
   aGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAGTTCATGAc                                                                                                                                    >  4:55014/1‑149 (MQ=255)
      gATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCt                                                                                                                                 >  4:292405/1‑149 (MQ=255)
         cAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAGTTCATGAccgt                                                                                                                                 >  6:344733/1‑143 (MQ=255)
         cAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAGTTCATGAccgt                                                                                                                                 <  5:344733/146‑4 (MQ=255)
          aaCGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAGTTCATGAccgtcga                                                                                                                              <  3:55014/148‑7 (MQ=255)
           aCGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGaa                                                                                                                            >  2:327516/1‑149 (MQ=255)
              gCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACgg                                                                                                                         <  3:50782/149‑1 (MQ=255)
                gtggtgGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGc                                                                                                                       <  1:327516/149‑1 (MQ=255)
                   gtggAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGcctc                                                                                                                    <  3:292405/149‑1 (MQ=255)
                       aGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTg                                                                                                                <  3:70942/149‑1 (MQ=255)
                         tCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTg                                                                                                                >  4:189082/1‑147 (MQ=255)
                           gATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAgg                                                                                                            >  2:37965/1‑149 (MQ=255)
                           gATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAgg                                                                                                            >  4:261329/1‑149 (MQ=255)
                            aTGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAgg                                                                                                            >  5:45039/1‑148 (MQ=255)
                                    ttGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGcctcc                                                                                                                   <  4:342125/133‑1 (MQ=255)
                                    ttGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGcctcc                                                                                                                   >  3:342125/1‑133 (MQ=255)
                                      gCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAgg                                                                                                 >  6:361721/1‑149 (MQ=255)
                                      gCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAg                                                                                                  <  5:234532/148‑1 (MQ=255)
                                        aGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGa                                                                                                                                     <  5:168039/111‑1 (MQ=255)
                                        aGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGa                                                                                                                                     >  6:168039/1‑111 (MQ=255)
                                        aGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGt                                                                                               >  1:128395/1‑149 (MQ=255)
                                        aGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGt                                                                                               >  1:214331/1‑149 (MQ=255)
                                        aGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGAGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATTCTCGACGGTCATGAACTCATGACGCGTCGAACGGGCCTCCTTGAATGCAAGATTGAGGGt                                                                                               <  3:46993/149‑1 (MQ=255)
                                              tGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTc                                                                                         <  7:205078/149‑1 (MQ=255)
                                              tGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTc                                                                                         <  6:45039/149‑1 (MQ=255)
                                               gCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTc                                                                                         >  1:132476/1‑148 (MQ=255)
                                                cTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGa                                                                                       <  3:256849/149‑1 (MQ=255)
                                                            gagaTTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGttt                                                                            <  3:189082/148‑1 (MQ=255)
                                                              gaTTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTaa                                                                          <  2:132476/148‑1 (MQ=255)
                                                                  ggCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATa                                                                      <  2:131928/148‑1 (MQ=255)
                                                                     gcCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACa                                                                        <  1:46932/143‑1 (MQ=255)
                                                                     gcCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACa                                                                        >  2:46932/1‑143 (MQ=255)
                                                                     gcCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCtt                                                                  >  5:99821/1‑149 (MQ=255)
                                                                      ccACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGc                                                                    >  4:120473/1‑146 (MQ=255)
                                                                      ccACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGc                                                                    <  3:120473/146‑1 (MQ=255)
                                                                            gcgcgcACAACGGTCGCGGCAGCCTCATTGTCAACGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCAAGACGCTTCGAACGGCCCTCCTTGAAGGCAAGATTGACGGTGACATCGAGCTAGCGGTTTAACATAGCTACACCTc                                                            >  8:24910/1‑148 (MQ=255)
                                                                             cgcgcAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCAt                                                          <  7:24910/149‑1 (MQ=255)
                                                                              gcgcAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCt                                                                   >  2:344108/1‑139 (MQ=255)
                                                                              gcgcAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCt                                                                   <  1:344108/139‑1 (MQ=255)
                                                                                   gAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATAccc                                                      >  4:160770/1‑147 (MQ=255)
                                                                                   gAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCaa                                                    <  2:128395/149‑1 (MQ=255)
                                                                                   gAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCaa                                                    <  2:214331/149‑1 (MQ=255)
                                                                                    aaCGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAg                                                   <  5:361721/149‑1 (MQ=255)
                                                                                     aCGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGt                                                  <  3:261329/149‑1 (MQ=255)
                                                                                                   cTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCTGTTTAACATAGCTTCACCTCAt                                                          <  8:302295/127‑1 (MQ=255)
                                                                                                   cTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCAt                                                          >  7:302295/1‑127 (MQ=255)
                                                                                                   cTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAAc                                    >  1:153141/1‑149 (MQ=255)
                                                                                                   cTCATTGTCAAGGAGTGCCAGCAGCAGAAGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCCCATACCCAAGTGGTCGGCGATTAAc                                    >  1:20674/1‑149 (MQ=255)
                                                                                                         gTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACTTCATACCCAAGTGGTCGGCGATTAACCGTcc                               <  1:37965/148‑1 (MQ=255)
                                                                                                         gTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCt                              >  4:316982/1‑149 (MQ=255)
                                                                                                          tCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCaaa                                                   <  2:201891/127‑2 (MQ=255)
                                                                                                          tCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCaaa                                                   >  1:201891/1‑126 (MQ=255)
                                                                                                          tCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCAGACCCAAGTGGTCGGCGATTAACCGTCCtt                             >  5:316892/1‑149 (MQ=255)
                                                                                                                aGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCCCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGAt                       <  2:20674/149‑1 (MQ=255)
                                                                                                                                  tCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTcaca                 <  6:234851/137‑1 (MQ=255)
                                                                                                                                  tCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTcaca                 >  5:234851/1‑137 (MQ=255)
                                                                                                                                     agcgTCATGAGTTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTCACag                >  3:208594/4‑135 (MQ=255)
                                                                                                                                     agcgTCATGAGTTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTCACag                <  4:208594/132‑1 (MQ=255)
                                                                                                                                     aCGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTCACAGAGTAGCGGATGCTg  <  6:99821/149‑1 (MQ=255)
                                                                                                                                           aTGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTaa                                                                          <  5:240947/71‑1 (MQ=255)
                                                                                                                                           aTGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTaa                                                                          >  6:240947/1‑71 (MQ=255)
                                                                                                                                                |                                                                                                                                         
GACAGGGATCAACGGCGTGGTGGAGTCGATGAACTCTTGCAGGTCATGCTTGAGCTTGTCGAGATTGGCGCCACAGGCGCGCAGAACGGTCGCGGCAGCCTCATTGTCAAGGAGTGCCAGCAGCAGATGCTCGACGGTCATGAACTCATGACGCTTCGAACGGGCCTCCTTGAAGGCAAGATTGAGGGTGACTTCGAGCTCGCGGTTTAACATAGCTTCACCTCATACCCAAGTGGTCGGCGATTAACCGTCCTTCTCGATTTCACAGAGTAGCGGATGCTG  >  NC_002947/4519307‑4519588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑