Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,521,320 G→A 6.9% P140S (CCA→TCA)  icd ← NADP(+)‑specific isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,521,3200GA 6.9% 171.5 / 2.0 58P140S (CCA→TCA) icdNADP(+)‑specific isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (28/26);  new base A (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GGTGACCCCCATTTCCTCCTTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGATCGATACCACATAATCACGCA  >  NC_002947/4521174‑4521457
                                                                                                                                                  |                                                                                                                                         
ggTGACCCCCATTTCCTCCTTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTgg                                                                                                                                           <  6:159669/147‑1 (MQ=255)
      ccccAATTCCTACTTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGACCGGCTTTTTCACCGGGCTTGGCaaaca                                                                                                                                     >  6:19973/1‑146 (MQ=255)
        ccATTTCCTCCTTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTg                                                                                                                                  >  1:341423/1‑148 (MQ=255)
               ctcctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACcaca                                                                                                                          <  5:19973/149‑1 (MQ=255)
                tcctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACAccc                                                                                                                                    <  1:95119/138‑1 (MQ=255)
                tcctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACAccc                                                                                                                                    >  2:95119/1‑138 (MQ=255)
                tcctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAg                                                                                                                         >  5:352358/1‑149 (MQ=255)
                tcctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAg                                                                                                                         >  3:220728/1‑149 (MQ=255)
                  ctTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCa                                                                                                                       >  3:146909/1‑149 (MQ=255)
                     gagaAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCg                                                                                                                    >  4:175637/1‑149 (MQ=255)
                       gaAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTgg                                                                                                                                           >  4:71828/1‑124 (MQ=255)
                       gaAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTgg                                                                                                                                           <  3:71828/124‑1 (MQ=255)
                       gaAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGc                                                                                                                  <  4:146909/149‑1 (MQ=255)
                         aaCTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCg                                                                                                                <  7:315888/149‑1 (MQ=255)
                          aCTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCg                                                                                                                >  3:76846/1‑148 (MQ=255)
                                           gCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTc                                                                                              >  7:271750/1‑149 (MQ=255)
                                           gCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTc                                                                                              <  8:271750/149‑1 (MQ=255)
                                            ccTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTc                                                                                              >  7:76344/1‑148 (MQ=255)
                                            ccTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGACACACCCTGGAACCACAGCCCCGGCCGCAGGCATACATACAggga                                                                                              >  3:272707/1‑146 (MQ=255)
                                            ccTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGACACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTc                                                                                              >  3:277416/1‑148 (MQ=255)
                                             cTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCa                                                                                            <  2:341423/149‑1 (MQ=255)
                                                gggCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGtt                                                                                         >  1:165641/1‑149 (MQ=255)
                                                gggCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGtt                                                                                         >  3:265060/1‑149 (MQ=255)
                                                gggCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGATTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGtt                                                                                         <  5:298560/149‑1 (MQ=255)
                                                     aCCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTgg                                                                                    <  4:265060/149‑1 (MQ=255)
                                                      cccGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGc                                                                                                           <  6:341678/125‑1 (MQ=255)
                                                      cccGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGc                                                                                                           >  5:341678/1‑125 (MQ=255)
                                                       ccGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCCCCGGCCGCAGGCATACATACAGGTCCAGTTGCTGgcg                                                                                  >  2:258351/1‑149 (MQ=255)
                                                       ccGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGgcg                                                                                  >  2:191627/1‑149 (MQ=255)
                                                           ccTTCCAGTCGATGTCGGCATAGATGTCTTCGGAGTTCTCGCGGTAGATCACCATGTCTACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACGACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAgg                                                                              <  4:220728/149‑1 (MQ=255)
                                                           ccTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAgg                                                                              <  1:258351/149‑1 (MQ=255)
                                                             ttCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGc                                                                            >  5:72903/1‑149 (MQ=255)
                                                                  cTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGt                                                                       <  6:352358/149‑1 (MQ=255)
                                                                     gATGCCGTCATAGCTGTCTTCTGAGGTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGACACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGa                                                                    <  4:272707/149‑1 (MQ=255)
                                                                     gATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGa                                                                    <  3:175637/149‑1 (MQ=255)
                                                                     gATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGACACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGa                                                                    <  4:277416/149‑1 (MQ=255)
                                                                        gCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAgg                                                                  <  8:76344/148‑1 (MQ=255)
                                                                          cGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAgg                                                                  >  2:129474/1‑146 (MQ=255)
                                                                          cGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGAAg                                                               <  1:129474/149‑1 (MQ=255)
                                                                                     tCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGcata                                                                                                        <  8:214444/97‑1 (MQ=255)
                                                                                     tCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGcata                                                                                                        >  7:214444/1‑97 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAgg                                                                                                >  1:220453/1‑103 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAgg                                                                                                <  2:220453/103‑1 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGa                                                                    <  5:15684/131‑1 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGa                                                                    >  6:15684/1‑131 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCAc                                                  >  8:247075/1‑149 (MQ=255)
                                                                                       ttCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCAc                                                  >  8:121723/1‑149 (MQ=255)
                                                                                         cGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAg                                                                                                             <  8:123899/88‑1 (MQ=255)
                                                                                         cGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAg                                                                                                             >  7:123899/1‑88 (MQ=255)
                                                                                                  cgcgGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTg                                       <  7:121723/149‑1 (MQ=255)
                                                                                                   gcgGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCa                                                                          >  4:261427/1‑113 (MQ=255)
                                                                                                   gcgGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCa                                                                          <  3:261427/113‑1 (MQ=255)
                                                                                                   gcgGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGt                                        <  4:76846/147‑1 (MQ=255)
                                                                                                    cgGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGt                                     <  1:191627/149‑1 (MQ=255)
                                                                                                         gATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAgg                                                                  <  3:187767/115‑1 (MQ=255)
                                                                                                         gATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAgg                                                                  >  4:187767/1‑115 (MQ=255)
                                                                                                               cATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCt                          >  3:236345/1‑149 (MQ=255)
                                                                                                                   tCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGa                       <  7:247075/148‑1 (MQ=255)
                                                                                                                    cGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGATc                     >  4:253065/1‑149 (MQ=255)
                                                                                                                       cGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGATCGAt                  <  4:236345/149‑1 (MQ=255)
                                                                                                                                       cACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGATCGATACCACATAATcacgca  >  7:204272/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
GGTGACCCCCATTTCCTCCTTGAGAAACTTGATCACCTTGTTCGCCTCGGGCGACCCGGCCTTCCACTCGATGCCGGCATAGATGTCTTCGGAGTTCTCGCGGAAGATCACCATGTCGACGTCGCCCGGCTTTTTCACCGGGCTTGGCACACCCTGGAACCACAGCACCGGCCGCAGGCATACATACAGGTCCAGTTGCTGGCGCAGGGCCACGTTGAGGGAGCGGATGCCGCCACCGACGGGCGTGGTCAGCGGGCCCTTGATCGATACCACATAATCACGCA  >  NC_002947/4521174‑4521457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑