Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,528,353	T→A	7.2%	intergenic (+28/‑48)	purB → / → PP_4017	adenylosuccinate lyase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,528,353	0	T	A	7.2%	202.7 / 4.3	69	intergenic (+28/‑48)	purB/PP_4017	adenylosuccinate lyase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/30);  new base A (2/3);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

AAGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGG  >  NC_002947/4528207‑4528494                                                                                                                                                   |                                                                                                                                              aaGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTgg                                                                                                                                             <  4:339497/149‑1 (MQ=255)    cgcTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCg                                                                                                                                          >  5:204692/1‑149 (MQ=255)     gctgctGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCgg                                                                                                                                         <  4:26555/149‑1 (MQ=255)             ccTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCGGGCGttgta                                                                                                                                 <  1:182822/149‑4 (MQ=255)               ttCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTTCCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCgg                                                                                                                                         <  5:111544/139‑1 (MQ=255)               ttCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTTCCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCAGGCCgg                                                                                                                                         >  6:111544/1‑139 (MQ=255)                     gACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCg                                                                                                                          >  7:126857/1‑148 (MQ=255)                      aCGGGCTGGACATGCCAGCCGTAGCCATGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCTGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGACCGGCCTGGCCGGGCGTTTTTATTCCCGTa                                                                                                                        <  2:249622/149‑1 (MQ=255)                       cGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTAc                                                                                                                       >  6:77236/1‑149 (MQ=255)                              gACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAAt                                                                                                                 >  6:232444/1‑148 (MQ=255)                                       gCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt                                                                                                       <  8:126857/149‑1 (MQ=255)                                          gAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGACCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCaa                                                                                                    >  2:37918/1‑149 (MQ=255)                                           aaGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAAt                                                                                                   <  5:77236/149‑1 (MQ=255)                                               cAAGGCCGATCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt                                                                                               <  6:204692/149‑1 (MQ=255)                                               cAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt                                                                                               <  5:232444/149‑1 (MQ=255)                                               cAAGGCCGAGCTCAAGCAGCTGACCCCCGCAACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGt                                                                                               >  1:343233/1‑149 (MQ=255)                                                aaGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGtt                                                                                              >  5:62936/1‑149 (MQ=255)                                                 aGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGtt                                                                                              >  4:177224/1‑148 (MQ=255)                                                  ggCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTACCGTACGAAAATTACGTTTTTTAAATAGGttt                                                                                             >  8:50680/1‑147 (MQ=255)                                                   gCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGaa                                                                                           <  3:177224/149‑1 (MQ=255)                                                      gAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACAt                                                                                        <  1:153811/149‑1 (MQ=255)                                                            aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACTCATCTAAGCTTAGCGTCGCGTACAACGCCCGGCCTGGCCGGGGGTTTTTATTCCCGTACGAAAATTACGTTTTTTCtata                                                                                                  <  5:349051/133‑1 (MQ=255)                                                            aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt                                                                                                       >  7:171880/1‑128 (MQ=255)                                                            aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtttttt                                                                                                       <  8:171880/128‑1 (MQ=255)                                                            aaGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTACCGTAAGAAAATTACGTTTTTTCAATa                                                                                                  >  6:349051/1‑133 (MQ=255)                                                              gcagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCt                                                                                 >  8:17947/1‑148 (MQ=255)                                                               cagcTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGa                                                                               >  4:100438/1‑149 (MQ=255)                                                                     aCCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCa                                                                                                     >  8:289573/1‑121 (MQ=255)                                                                     aCCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCa                                                                                                     <  7:289573/121‑1 (MQ=255)                                                                      cccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCa                                                                        >  4:205630/1‑149 (MQ=255)                                                                      cccccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCa                                                                        <  6:62936/149‑1 (MQ=255)                                                                      cccccGCAACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCa                                                                        <  2:343233/149‑1 (MQ=255)                                                                         ccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTg                                                                     >  5:127724/1‑149 (MQ=255)                                                                         ccGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGGTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCCCTg                                                                     >  3:286946/1‑149 (MQ=255)                                                                            ctacctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAg                                                                  >  1:53962/1‑149 (MQ=255)                                                                            ctacctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAg                                                                  <  3:289820/149‑1 (MQ=255)                                                                               cctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCt                                                                                 >  8:253387/1‑131 (MQ=255)                                                                               cctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctg                                                               <  2:53962/149‑1 (MQ=255)                                                                               cctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCCGGCGATTTTATTCCCGTACGAAAATTACGtt                                                                                                           >  5:356005/1‑105 (MQ=255)                                                                               cctacATCGGTAAAGCGGCAGCCGATGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGtt                                                                                                           <  6:356005/105‑1 (MQ=255)                                                                                ctacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTACCGTACGAAAATTACGTTTTTTGAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctgc                                                              >  7:283621/1‑149 (MQ=255)                                                                                 tacATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctgct                                                             >  5:186163/1‑149 (MQ=255)                                                                                     tCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTcggc                                                         <  7:50680/149‑1 (MQ=255)                                                                                        gTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGc                                                                    >  8:166541/1‑135 (MQ=255)                                                                                        gTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGc                                                                    <  7:166541/135‑1 (MQ=255)                                                                                          aaCGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCAt                                                    >  1:253561/1‑149 (MQ=255)                                                                                           aCGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCAtc                                                   <  3:205630/149‑1 (MQ=255)                                                                                                       aGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGaa                                       >  2:334280/1‑149 (MQ=255)                                                                                                        ggCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAAt                                      <  6:35608/149‑1 (MQ=255)                                                                                                          ccAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAAtt                                     >  4:252568/1‑148 (MQ=255)                                                                                                          ccAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTc                                    <  1:334280/149‑1 (MQ=255)                                                                                                           cAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCgg                                               >  8:81498/1‑137 (MQ=255)                                                                                                           cAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCgg                                               <  7:81498/137‑1 (MQ=255)                                                                                                                      aaGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTGCCGTACGAAAATTACGGTTTTTGAATAGGTTGAACATGAATCCTGATACTGCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTGATGCGCGactac                        >  7:280926/1‑149 (MQ=255)                                                                                                                      aaGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctgct                                                             <  4:86904/112‑1 (MQ=255)                                                                                                                      aaGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAgctgct                                                             >  3:86904/1‑112 (MQ=255)                                                                                                                      aaGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGactac                        >  6:295049/1‑149 (MQ=255)                                                                                                                       aGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGac                           <  3:100438/145‑1 (MQ=255)                                                                                                                         cTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTgg                     <  6:127724/149‑1 (MQ=255)                                                                                                                            aGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCa                   <  7:17947/148‑1 (MQ=255)                                                                                                                                tCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCagaag               >  3:332717/1‑148 (MQ=255)                                                                                                                                       aaaCGCCCGGCCAAGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCaga                 <  1:6318/138‑1 (MQ=255)                                                                                                                                       aaaCGCCCGGCCAAGCCGGGCGTTTTTATTACCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCaga                 >  2:6318/2‑139 (MQ=255)                                                                                                                                        aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTAGGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgc        <  7:188152/147‑1 (MQ=255)                                                                                                                                        aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCtgaa                <  7:292995/139‑1 (MQ=255)                                                                                                                                        aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCtgaa                >  8:292995/1‑139 (MQ=255)                                                                                                                                        aaCGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCgc        >  8:188152/1‑147 (MQ=255)                                                                                                                                           gCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGctgctg   <  5:295049/149‑1 (MQ=255)                                                                                                                                             ccGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTgg  >  2:334303/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                              AAGCGCTGCTGACCTTCATCGACGGCCTGGACATGCCAGCCGAAGCCAAGGCCGAGCTCAAGCAGCTGACCCCCGCTACCTACATCGGTAACGCGGCAGCCCAGGCCAAACGCATCTAAGCTGAGCGTCGCGTACAACGCCCGGCCTGGCCGGGCGTTTTTATTCCCGTACGAAAATTACGTTTTTTCAATAGGTTGAACATGAATCCTGATACTCCACTGCAGCTGCTCGGCGGCATCTCGGCCCGCGAATTCATGCGCGACTACTGGCAGAAGAAGCCGCTGCTGG  >  NC_002947/4528207‑4528494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑