Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,536,024 T→C 10.5% intergenic (+551/‑264) PP_4022 → / → PP_4024 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,536,0240TC10.5% 97.5 / 2.4 38intergenic (+551/‑264)PP_4022/PP_4024hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base T (17/17);  new base C (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCCAGCCGAATGGCTGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATCGCGGCGCTGACGGTTT  >  NC_002947/4535878‑4536170
                                                                                                                                                   |                                                                                                                                                  
cATGCCAGCCGAATGGCTGCACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTGCGGCGGCCCGCCCAAGGCG‑CCGCTGGCTGCCGAGGCCCCGATCAAGAAAAAGTGGCCAGTCCCGCAAGGCTTCCCCCCgg                                                                                                                                                  >  2:333825/1‑149 (MQ=255)
cATGCCAGCCGAATGGCTGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCCgg                                                                                                                                                  >  2:339272/1‑149 (MQ=255)
     cAGCCGAATGGCTGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTc                                                                                                                                             >  5:335535/1‑149 (MQ=255)
       gCCGAATGGCTGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGTCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTa                                                                                                                                           <  3:149565/149‑1 (MQ=255)
                 tGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCg                                                                                                                                   >  3:311735/1‑147 (MQ=255)
                           gcggcCCGTGAGCGTCTTCGCCTGTGGCCTCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCGGGATGCCGAGGCACCGATCAAGAAAACGGGGCACGTCCCGCACGGCTTCACCCCGGGCTTCTACGACGGCGGCGCCGGCCCgg                                                                                                                       <  1:333825/146‑1 (MQ=255)
                            ctgcCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCCGGGCTTCTACGACGGCGTAGCCGGCCCgg                                                                                                                       <  1:339272/148‑1 (MQ=255)
                                   tGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCGTCCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCgg                                                                                                                >  1:266557/1‑149 (MQ=255)
                                             cGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCggctac                                                                                                       <  2:266557/147‑6 (MQ=255)
                                             cGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTg                                                                                                       <  1:304419/147‑1 (MQ=255)
                                                   tgGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGt                                                                                                 <  7:157323/147‑1 (MQ=255)
                                                   tgGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCa                                                                                               <  7:206269/149‑1 (MQ=255)
                                                           aCCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCTATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTgg                                                                                                      <  3:37120/134‑1 (MQ=255)
                                                           aCCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCTATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTgg                                                                                                      >  4:37120/1‑134 (MQ=255)
                                                                  gttCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCctgctgctg                                                                                 >  1:190948/1‑148 (MQ=255)
                                                                              gCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTAc                                                                              >  6:280271/1‑139 (MQ=255)
                                                                              gCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTAc                                                                              <  5:280271/139‑1 (MQ=255)
                                                                                 cAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTgg                                                                 <  4:311735/149‑1 (MQ=255)
                                                                                                 tGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAgcggcg                                                  >  1:38158/1‑149 (MQ=255)
                                                                                                     gATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCACCGGCGTGgc                                              >  8:93922/1‑149 (MQ=255)
                                                                                                          aCCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGgcgcg                                           >  7:232852/1‑147 (MQ=255)
                                                                                                          aCCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCgca                                         <  2:190948/149‑1 (MQ=255)
                                                                                                            cGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCgcag                                        >  3:186474/1‑148 (MQ=255)
                                                                                                             gATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCa                                                                                               <  5:350192/92‑1 (MQ=255)
                                                                                                             gATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCa                                                                                               >  6:350192/1‑92 (MQ=255)
                                                                                                               tCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGgcgcgc                                          >  5:56129/1‑143 (MQ=255)
                                                                                                               tCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGgcgcgc                                          <  6:56129/143‑1 (MQ=255)
                                                                                                               tCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCAt                                     >  8:144672/1‑148 (MQ=255)
                                                                                                                          tGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAg                         <  6:335535/149‑1 (MQ=255)
                                                                                                                                    cGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACAt                   >  5:21332/1‑145 (MQ=255)
                                                                                                                                    cGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACAt                   <  6:21332/145‑1 (MQ=255)
                                                                                                                                    cGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACAt                   <  6:26534/145‑1 (MQ=255)
                                                                                                                                    cGCAAGGCTTCACCCTGGGCTTCTACGACGGAGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACAt                   >  5:26534/1‑145 (MQ=255)
                                                                                                                                      cAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATCgcggc             <  1:48700/149‑1 (MQ=255)
                                                                                                                                      cAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATCgcggc             <  2:38158/149‑1 (MQ=255)
                                                                                                                                            ttCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATcgc                >  6:160969/1‑140 (MQ=255)
                                                                                                                                            ttCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATcgc                <  5:160969/140‑1 (MQ=255)
                                                                                                                                                 ccTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATCGCGGCGCTGACGGttt  >  2:55205/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATGCCAGCCGAATGGCTGAACAAGATGCTGCCAGTGATCGTCTTCGCCTGTGGCATCTACCTGTTGTTCGGCGGCACGCCCAAGGCG‑CCGCTGGATGCCGATGCACCGATCAAGAAAAAGTGGCAAGTCCCGCAAGGCTTCACCCTGGGCTTCTACGACGGCGTAGCCGGCCCGGGTACCGGGGCGTTCTGGACGGTCAGTACCCTGCTGCTGTACCCCATCGACCTGGTACGCGCCAGCGGCGTGGCGCGCAGCATGAACTTCGTCAGCAACATCGCGGCGCTGACGGTTT  >  NC_002947/4535878‑4536170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑