Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,541,836 C→T 6.3% Q185* (CAG→TAG) ‡ nhaB → Na(+)/H(+) antiporter NhaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,541,8360CT 6.3% 237.9 / 3.5 79Q185* (CAG→TAG) ‡nhaBNa(+)/H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base C (36/38);  new base T (2/3);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

TACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCACGAGAT  >  NC_002947/4541693‑4541984
                                                                                                                                               |                                                                                                                                                    
tACTGTGGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGc                                                                                                                                                   >  4:59046/1‑147 (MQ=255)
  cTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAg                                                                                                                                                <  8:181538/148‑1 (MQ=255)
  cTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAg                                                                                                                                                <  3:132152/148‑1 (MQ=255)
     tCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCg                                                                                                                                             <  2:149906/148‑1 (MQ=255)
              cTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACTTGCTGGTCGCTCTccagt                                                                                                                                    <  1:268427/148‑4 (MQ=255)
                aTCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCAcc                                                                                                                                 >  7:21536/1‑149 (MQ=255)
                    gcgTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCggg                                                                                                                              >  5:227218/1‑148 (MQ=255)
                      gTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCggg                                                                                                                              >  8:21022/1‑146 (MQ=255)
                           tcGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGTGAGCGACTTGCTGGTCGCTCTcca                                                                                                                                      <  6:239863/133‑2 (MQ=255)
                           tcGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGTGAGCGACTTGCTGGTCGCTCTcca                                                                                                                                      >  5:239863/1‑132 (MQ=255)
                             gATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTc                                                                                                                      <  3:59046/147‑1 (MQ=255)
                              aTGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAc                                                                                                                   <  8:160410/149‑1 (MQ=255)
                              aTGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAc                                                                                                                   >  2:290776/1‑149 (MQ=255)
                              aTGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGAc                                                                                                                   <  8:21536/149‑1 (MQ=255)
                                 cccTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCgg                                                                                                                               <  2:178407/134‑1 (MQ=255)
                                 cccTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCgg                                                                                                                               >  1:178407/1‑134 (MQ=255)
                                  ccTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGt                                                                                                               >  1:149506/1‑149 (MQ=255)
                                  ccTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGt                                                                                                               >  6:89808/1‑149 (MQ=255)
                                   cTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGtt                                                                                                              >  8:17495/1‑149 (MQ=255)
                                      aCCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGAAAGCGCACTGGAGAGCGCCCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCcg                                                                                                           >  8:246730/1‑149 (MQ=255)
                                               gcggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGGTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCt                                                                                                  <  8:102179/149‑1 (MQ=255)
                                                cggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTg                                                                                                 >  6:9041/1‑149 (MQ=255)
                                                 ggTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGc                                                                                                >  1:240524/1‑149 (MQ=255)
                                                      tcatcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGc                                                                                           <  7:17495/149‑1 (MQ=255)
                                                      tcatcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGc                                                                                           <  1:276157/149‑1 (MQ=255)
                                                        atcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCct                                                                                         >  3:15047/1‑149 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTc                                                                                                             <  7:185971/128‑1 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTc                                                                                                             >  8:185971/1‑128 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGc                                                                                           <  1:134564/146‑1 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGc                                                                                           >  2:134564/1‑146 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctg                                                                                        >  6:290771/1‑149 (MQ=255)
                                                         tcaGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctg                                                                                        >  6:310380/1‑149 (MQ=255)
                                                           aGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctgct                                                                                      <  4:15047/149‑1 (MQ=255)
                                                           aGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctgc                                                                                       <  6:227218/148‑1 (MQ=255)
                                                            gcgcTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTcc                                                                                                   <  3:135089/135‑1 (MQ=255)
                                                            gcgcTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTcc                                                                                                   >  4:135089/1‑135 (MQ=255)
                                                                   ggTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATg                                                                              <  1:130649/149‑1 (MQ=255)
                                                                    gTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATgg                                                                             <  2:240524/149‑1 (MQ=255)
                                                                    gTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATgg                                                                             >  5:83395/1‑149 (MQ=255)
                                                                     tCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGgc                                                                            >  1:49221/1‑149 (MQ=255)
                                                                       ggCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGgcgc                                                                          >  8:3619/1‑149 (MQ=255)
                                                                       ggCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGgcgc                                                                          <  2:149506/149‑1 (MQ=255)
                                                                         cTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCt                                                                                                  <  2:279656/123‑1 (MQ=255)
                                                                         cTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCt                                                                                                  >  1:279656/1‑123 (MQ=255)
                                                                          ttCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                       >  2:262221/1‑149 (MQ=255)
                                                                            cTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgtg                                                                      <  5:9041/148‑1 (MQ=255)
                                                                                   gtgtACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGc                                                              >  4:136039/1‑149 (MQ=255)
                                                                                        ccaccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTgg                                                         >  7:226950/1‑149 (MQ=255)
                                                                                         caccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTggg                                                        >  3:299821/1‑149 (MQ=255)
                                                                                         caccGCATCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                       <  8:77667/134‑1 (MQ=255)
                                                                                         caccGCATCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCtgt                                                                       >  7:77667/1‑134 (MQ=255)
                                                                                          accGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCtcct                                                                                                   <  5:251547/105‑4 (MQ=255)
                                                                                          accGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCtcct                                                                                                   >  6:251547/1‑102 (MQ=255)
                                                                                          accGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCctgc                                                                                       <  8:95069/117‑1 (MQ=255)
                                                                                           ccGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggcg                                                      <  2:49221/149‑1 (MQ=255)
                                                                                             gcgtcgcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTgg                                                                     <  6:12240/132‑1 (MQ=255)
                                                                                             gcgtcgcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTgg                                                                     >  5:12240/1‑132 (MQ=255)
                                                                                             gcgtcgcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggcggc                                                    <  1:290776/149‑1 (MQ=255)
                                                                                                  gcgtcCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTg                                               <  6:83395/149‑1 (MQ=255)
                                                                                                       cGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGt                                          <  7:21022/149‑1 (MQ=255)
                                                                                                        ggggCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGtt                                         <  8:226950/149‑1 (MQ=255)
                                                                                                            ccAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggtg                                     <  5:310380/149‑1 (MQ=255)
                                                                                                            ccAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggtg                                     <  7:35388/149‑1 (MQ=255)
                                                                                                                tCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggtgg                                    <  6:270950/146‑1 (MQ=255)
                                                                                                                tCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTtggtgg                                    >  5:270950/1‑146 (MQ=255)
                                                                                                                tCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGc                                  >  6:337687/1‑148 (MQ=255)
                                                                                                                       gAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCa                          <  1:262221/149‑1 (MQ=255)
                                                                                                                             aGCGCGCTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggc                                                       <  4:206725/114‑1 (MQ=255)
                                                                                                                             aGCGCGCTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggc                                                       >  3:206725/1‑114 (MQ=255)
                                                                                                                              gcgcACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctg                   >  4:61556/1‑149 (MQ=255)
                                                                                                                               cgcACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctgc                  <  5:89808/149‑1 (MQ=255)
                                                                                                                                gcACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctgc                  >  7:186984/1‑148 (MQ=255)
                                                                                                                                gcACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACctgc                  <  8:186984/148‑1 (MQ=255)
                                                                                                                                    tGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCGTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCGTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATc             >  6:129093/1‑149 (MQ=255)
                                                                                                                                      gtgagCGACTTGCTGGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggcggc                                                    <  5:150514/106‑1 (MQ=255)
                                                                                                                                      gtgagCGACTTGCTGGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGggcggc                                                    >  6:150514/3‑108 (MQ=255)
                                                                                                                                      gagaGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCg            <  5:337687/148‑1 (MQ=255)
                                                                                                                                        gagCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGc          >  2:1558/1‑148 (MQ=255)
                                                                                                                                            gACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCACga     <  5:290771/149‑1 (MQ=255)
                                                                                                                                               cAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCACGAGAt  >  2:276672/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TACTGTCGGCGTTTCTATCCGCGTTTCTCGATGCCCTGACCGTCACGGCGGTGATCATCAGCGCTGCGGTCGGCTTCTACGCGGTGTACCACCGCGTCGCGTCCGGGGCCAATCCGCGCGAGGACAGCGCACTGGAGAGCGACCAGCAAGTCGCTCACCTGCACCGGGAAGACCTCGACCAGTTCCGCGCCTTCCTGCGTAGCCTGCTGATGCATGGCGCTGTGGGTACCGCCCTGGGCGGCGTTTGCACGTTGGTGGGCGAGCCGCAGAACCTGCTGATCGGCCACGAGAT  >  NC_002947/4541693‑4541984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑