Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 86,240 G→T 10.2% L160M (CTG→ATG)  PP_0075 ← choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294786,2400GT10.2% 145.0 / 4.2 48L160M (CTG→ATG) PP_0075choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base T (3/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCG  >  NC_002947/86095‑86382
                                                                                                                                                 |                                                                                                                                               
aGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGc                                                                                                                                              <  6:316801/149‑1 (MQ=255)
  gCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGccc                                                                                                                                            >  8:141572/1‑148 (MQ=255)
      ggCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGcc                                                                                                                                        >  1:176019/1‑149 (MQ=255)
             gTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcag‑ccag                                                                                                                                <  7:141572/149‑4 (MQ=255)
                          tttGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑AAGGTTGGGTAGCTGGc                                                                                                                   >  5:362248/1‑149 (MQ=255)
                             gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGtt                                                                                                                              <  8:8689/135‑1 (MQ=255)
                             gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGtt                                                                                                                              >  7:8689/1‑135 (MQ=255)
                               aGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAg                                                                                                              <  6:92284/149‑1 (MQ=255)
                                ggCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAgg                                                                                                             <  2:176019/149‑1 (MQ=255)
                                  acGCACCGATCACGACGGTGGCCAGGCCAAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGTGCCATGCCCAG‑CAGGTTGGTTAGCTGGCCCAAGGCa                                                                                                           <  2:242315/148‑1 (MQ=255)
                                       ccGATCACGACGGGGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGAc                                                                                                      <  7:194490/149‑1 (MQ=255)
                                               gacgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                              >  8:111051/1‑149 (MQ=255)
                                                             ccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                       <  6:21602/126‑1 (MQ=255)
                                                             ccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                       >  5:21602/1‑126 (MQ=255)
                                                                 cagcaAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                            <  7:111051/149‑1 (MQ=255)
                                                                       ggaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGaa                                                                                 <  2:3517/138‑1 (MQ=255)
                                                                       ggaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGaa                                                                                 >  1:3517/1‑138 (MQ=255)
                                                                            gtaggtCGACCTCGCCTTGGTGGCTGGCCAGGTCCTGCAGGGTCATCAGCGCGGTTGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCGCAAGGCAATGACGATGGCGGCACCCAg                                                                                       <  2:106416/127‑1 (MQ=255)
                                                                            gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAg                                                                                       >  1:106416/1‑127 (MQ=255)
                                                                              aggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATg                                                               >  2:234209/1‑149 (MQ=255)
                                                                                         gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGt                                                    >  7:143535/1‑149 (MQ=255)
                                                                                         gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGt                                                    >  6:221577/1‑149 (MQ=255)
                                                                                           cTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                   >  4:164685/1‑148 (MQ=255)
                                                                                             gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                              >  5:322796/1‑103 (MQ=255)
                                                                                             gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                              <  6:322796/103‑1 (MQ=255)
                                                                                             gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACg                                                                    >  5:331044/1‑129 (MQ=255)
                                                                                             gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACg                                                                    <  6:331044/129‑1 (MQ=255)
                                                                                                  gctggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgcg                                           >  7:190352/1‑149 (MQ=255)
                                                                                                     ggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAg                                                                                <  7:311981/109‑1 (MQ=255)
                                                                                                     ggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAg                                                                                >  8:311981/1‑109 (MQ=255)
                                                                                                            tCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAgcg                                 <  1:234209/149‑1 (MQ=255)
                                                                                                                 cACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                              >  6:132044/1‑147 (MQ=255)
                                                                                                                 cACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                              >  8:249168/1‑147 (MQ=255)
                                                                                                                   cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGc                          <  8:143535/149‑1 (MQ=255)
                                                                                                                     gTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                   <  5:192716/122‑1 (MQ=255)
                                                                                                                     gTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                   >  6:192716/1‑122 (MQ=255)
                                                                                                                        atcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGag                     >  8:114547/1‑149 (MQ=255)
                                                                                                                            gcgcGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagc                 <  6:327005/149‑1 (MQ=255)
                                                                                                                              gcgGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagcag               <  7:192805/149‑1 (MQ=255)
                                                                                                                                   caacctgctggGCATGGCCCTGCCCAG‑CCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcag                  >  2:133494/7‑141 (MQ=255)
                                                                                                                                   caacctgctggGCATGGCCCTGCCCAG‑CCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcag                  <  1:133494/135‑1 (MQ=255)
                                                                                                                                      ctggctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGaaaa       >  3:274834/1‑149 (MQ=255)
                                                                                                                                        tgctggGCATGGCCCTGCCCAGCCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAAt      >  6:66732/2‑149 (MQ=255)
                                                                                                                                         gctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGATAAt      <  8:82541/147‑1 (MQ=255)
                                                                                                                                         gctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAAt      <  8:190352/147‑1 (MQ=255)
                                                                                                                                          ctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagc                    >  3:233316/1‑132 (MQ=255)
                                                                                                                                          ctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagc                    <  4:233316/132‑1 (MQ=255)
                                                                                                                                           tggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATgccg  >  1:115261/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGGCTCGGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCG  >  NC_002947/86095‑86382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑