| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 435,331 | G→A | 7.0% | intergenic (‑132/‑318) | glcB ← / → PP_0357 | malate synthase G/ACT domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 435,331 | 0 | G | A | 7.0% | 163.3 / 2.5 | 57 | intergenic (‑132/‑318) | glcB/PP_0357 | malate synthase G/ACT domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (23/30); new base A (2/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
AACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGC > NC_002947/435185‑435467 | aaCGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCggg < 7:11381/149‑1 (MQ=255) aCGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGc < 4:116033/149‑1 (MQ=255) cGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCggg > 5:294144/1‑147 (MQ=255) cAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGc < 6:294144/149‑1 (MQ=255) cAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGc < 2:4008/149‑1 (MQ=255) cAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCCGCCAGCCTGTCTGGCAGGc > 7:173067/1‑142 (MQ=255) cAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCCGCCAGCCTGTCTGGCAGGc < 8:173067/142‑1 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcaca < 2:112138/148‑1 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcaca < 4:63248/148‑1 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcac < 6:126486/147‑1 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcac < 5:36530/147‑1 (MQ=255) gcCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAg > 2:29042/1‑149 (MQ=255) tCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGgcg > 6:144089/1‑149 (MQ=255) ctGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGgc < 1:229206/143‑1 (MQ=255) ctGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGgc > 2:229206/1‑143 (MQ=255) tGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCtt < 4:178922/147‑1 (MQ=255) tGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTgg > 8:220394/1‑149 (MQ=255) tCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTgg > 5:1393/1‑146 (MQ=255) tCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTAGGGGTGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTgg > 6:162414/1‑146 (MQ=255) aGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGa > 4:287638/1‑148 (MQ=255) tATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATc < 3:287638/149‑1 (MQ=255) gCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAAGCGCCTTGGCAGACATCCAAac < 7:220394/148‑1 (MQ=255) ctctTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAgg < 7:194970/126‑1 (MQ=255) ctctTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAgg > 8:194970/1‑126 (MQ=255) ctGTATTCAGCCTGTAGCTCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTTCGTATTTTCACATGCGCCTTGGCAGACATCCAAacgac < 2:288411/145‑1 (MQ=255) ctGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtt < 4:161382/148‑1 (MQ=255) tGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt > 2:246718/1‑149 (MQ=255) tATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt < 6:219643/147‑1 (MQ=255) tATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt < 5:144089/147‑1 (MQ=255) ttAAGCCTGTAGCGCTTCTCTAAACACGGCACGAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa < 4:30867/148‑1 (MQ=255) cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcaca < 8:276543/102‑1 (MQ=255) cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTcaca > 7:276543/1‑102 (MQ=255) cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACgg > 4:146621/1‑149 (MQ=255) gcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGAGCCACgg > 2:93316/1‑148 (MQ=255) ttCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTAtt < 4:51029/93‑1 (MQ=255) ttCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTAtt > 3:51029/1‑93 (MQ=255) aaCACGGCACCAGTGCATGCCCCCGGGGTTGCTTAGGGGTGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtactac < 5:162414/147‑1 (MQ=255) acGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATg > 5:326400/1‑147 (MQ=255) cAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAg > 4:32402/1‑149 (MQ=255) gTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGc < 6:326400/149‑1 (MQ=255) cGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATc < 1:29042/149‑1 (MQ=255) ggTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa < 6:1393/149‑1 (MQ=255) gTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGAc < 3:32402/149‑1 (MQ=255) ttGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa < 4:36423/143‑1 (MQ=255) ttGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa > 3:36423/1‑143 (MQ=255) gggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa > 3:30234/1‑139 (MQ=255) gggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa < 4:30234/139‑1 (MQ=255) cTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTAt > 5:213249/1‑149 (MQ=255) tGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATg > 3:308993/1‑149 (MQ=255) ggCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCggg < 5:20837/114‑1 (MQ=255) ggCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCggg > 6:20837/1‑114 (MQ=255) cccgCCAGCCTGTCTGGCAGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCATACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa < 1:233579/120‑1 (MQ=255) cccgCCAGCCTGTCTGGCAGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCATACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa > 2:233579/4‑123 (MQ=255) ccTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGAccaacg > 5:92506/1‑145 (MQ=255) ccTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGGCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGAccaac < 6:92506/148‑4 (MQ=255) gCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGt > 6:317517/1‑149 (MQ=255) gCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGt > 3:90794/1‑149 (MQ=255) aGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc < 5:317517/149‑1 (MQ=255) aGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc < 4:90794/149‑1 (MQ=255) | AACGTATCCAGTCATTGCTTGCCTCACTCTGCTCAGCTATTTCGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGC > NC_002947/435185‑435467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |