Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,578,141 C→G 10.8% S657T (AGC→ACC)  glgB ← 1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,578,1410CG10.8% 152.4 / 5.4 53S657T (AGC→ACC) glgB1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base C (28/19);  new base G (4/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGATTGCCGACATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCCCACCAGGC  >  NC_002947/4577997‑4578279
                                                                                                                                                |                                                                                                                                          
ccGAGATTGCCGACATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCgctgct                                                                                                                                        >  8:94986/1‑149 (MQ=255)
ccGAGATTGCCGACATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCgctgct                                                                                                                                        >  2:238826/1‑149 (MQ=255)
 cGAGATTGCCGACATTCGACCAGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCgctgctg                                                                                                                                       >  7:125455/1‑149 (MQ=255)
          cGACATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAgcg                                                                                                                               >  1:333652/1‑148 (MQ=255)
            aCATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAg                                                                                                                            >  7:295738/1‑149 (MQ=255)
            aCATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCGGCTCCCGCGCAg                                                                                                                            >  4:4157/1‑149 (MQ=255)
               ttCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGc                                                                                                                           >  6:256586/1‑147 (MQ=255)
                 cGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAg                                                                                                                        <  1:154519/148‑1 (MQ=255)
                      cGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTac                                                                                                                  <  8:295738/149‑1 (MQ=255)
                       ggCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTaca                                                                                                                 <  8:125455/149‑1 (MQ=255)
                       ggCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTaca                                                                                                                 <  6:215571/149‑1 (MQ=255)
                         cATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACg                                                                                                               <  7:130758/149‑1 (MQ=255)
                                   ggCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTgc                                                                                                      <  2:182000/148‑1 (MQ=255)
                                   ggCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTgc                                                                                                      <  2:333652/148‑1 (MQ=255)
                                           tGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCa                                                                                             >  5:113058/1‑149 (MQ=255)
                                                 gAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCAGTTCTGCGCGTCGTCACCGAt                                                                                        >  2:232455/1‑148 (MQ=255)
                                                  aaGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATc                                                                                       >  7:327429/1‑148 (MQ=255)
                                                        cTGCCAACGCTCGCGGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACt                                                                                >  7:299394/1‑149 (MQ=255)
                                                        cTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCAc                                                                                 <  5:256586/148‑1 (MQ=255)
                                                          gCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTacac                                                                                                                >  3:41791/1‑115 (MQ=255)
                                                          gCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTacac                                                                                                                <  4:41791/115‑1 (MQ=255)
                                                           ccAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTCCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCCTCACCCATCAACCAcaga                                                                              >  4:235804/1‑145 (MQ=255)
                                                             aaCGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGaaa                                                                           <  6:113058/149‑1 (MQ=255)
                                                                     ccGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCgg                                                                   <  8:203994/149‑1 (MQ=255)
                                                                        aaCGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCCGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTg                                                                >  8:208900/1‑149 (MQ=255)
                                                                        aaCGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGTCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTg                                                                >  8:188957/1‑149 (MQ=255)
                                                                              aCGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATc                                                          >  7:280385/1‑149 (MQ=255)
                                                                                   aaTGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCt                                                     >  1:63081/1‑149 (MQ=255)
                                                                                        ggTAACCGTCAGGGGGTACCGGGGTGAAGTTGGCCAGCACCAGCACCGGCTCGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTGGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGc                                                <  3:235804/149‑1 (MQ=255)
                                                                                         gTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGc                                                >  1:74608/1‑148 (MQ=255)
                                                                                         gTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGc                                                >  6:207970/1‑148 (MQ=255)
                                                                                          tAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCaa                                              >  7:84942/1‑149 (MQ=255)
                                                                                              ccTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCACCCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGcc                                          >  6:25624/1‑149 (MQ=255)
                                                                                              ccTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  >  3:204772/1‑141 (MQ=255)
                                                                                              ccTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  <  4:204772/141‑1 (MQ=255)
                                                                                                tCACGGGGTACCGGGGTAAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCgg                                        >  2:285888/1‑149 (MQ=255)
                                                                                                   cGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGctc                                     <  8:299394/149‑1 (MQ=255)
                                                                                                          ccGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTcgcg                               <  7:208900/148‑1 (MQ=255)
                                                                                                                     ttGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGtt                   <  8:280385/149‑1 (MQ=255)
                                                                                                                       ggCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGc                                                      >  2:298367/1‑112 (MQ=255)
                                                                                                                       ggCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGc                                                      <  1:298367/112‑1 (MQ=255)
                                                                                                                         ccaccaccaGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAgg               <  2:63081/149‑1 (MQ=255)
                                                                                                                           tggagcagcagCGGCGAGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTc             >  6:256711/6‑149 (MQ=255)
                                                                                                                             caccaGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  >  2:286695/1‑110 (MQ=255)
                                                                                                                             caccaGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  <  1:286695/110‑1 (MQ=255)
                                                                                                                             caccaGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGc           >  5:272394/1‑149 (MQ=255)
                                                                                                                              accaGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGcc          >  2:222578/1‑149 (MQ=255)
                                                                                                                               gcagcagCGGCGAGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCTCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTcg                                 <  2:130027/124‑1 (MQ=255)
                                                                                                                               gcagcagCGGCGAGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTcg                                 >  1:130027/2‑125 (MQ=255)
                                                                                                                               gcagcagCGGCGAGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGcc          >  6:353923/2‑148 (MQ=255)
                                                                                                                                cagcagCGGCGAGCCGGTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCcca        >  8:83050/1‑149 (MQ=255)
                                                                                                                                caGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCcca        >  4:146613/1‑149 (MQ=255)
                                                                                                                                  gCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCccac       >  2:302500/1‑148 (MQ=255)
                                                                                                                                      cGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCCCACCAGgc  >  6:294320/1‑149 (MQ=255)
                                                                                                                                          tCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  >  6:8615/1‑97 (MQ=255)
                                                                                                                                          tCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGt                                                  <  5:8615/97‑1 (MQ=255)
                                                                                                                                            gccgcTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCccacca     <  4:137256/140‑1 (MQ=255)
                                                                                                                                            gccgcTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTACGCGGTACAGGCGGTTGAGGTCGCccacca     >  3:137256/1‑140 (MQ=255)
                                                                                                                                                |                                                                                                                                          
CCGAGATTGCCGACATTCGACCCGGCATACAGCTCGGCATCACTGTTCAGAAGCTCCTGCCAACGCTCGCCGAACGGCACGCCAATGCGGTAACCCTCACGGGGTACCGGGGTGAAGTTGGCCACCACCAGCACCGGCTCGCCGCTGCTGCTCCAGCGCAGCCAGGCGTACACGCTGTTCTGCGCGTCGTCACCGATCAACCACTGGAAACCCTGCGGCTGGCAATCCTGCTCGTGCAACGCCGGCTCTTCGCGGTACAGGCGGTTGAGGTCGCCCACCAGGC  >  NC_002947/4577997‑4578279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑