Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,615,343 G→A 9.3% V572M (GTG→ATG) ‡ PP_4085 → rhs family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,615,3430GA 9.3% 177.4 / 6.4 64V572M (GTG→ATG) ‡PP_4085rhs family protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base A (2/4);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

TAGCCATCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCT  >  NC_002947/4615195‑4615489
                                                                                                                                                    |                                                                                                                                                  
tAGCCATCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAg                                                                                                                                                    >  5:361286/1‑149 (MQ=255)
      tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTc                                                                                                                                              <  4:282591/149‑1 (MQ=255)
      tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAAACCGATCACGAATGGCGAGTGATTc                                                                                                                                              >  1:137625/1‑149 (MQ=255)
             gCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTg                                                                                                                                       >  3:196347/1‑149 (MQ=255)
              cTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTgg                                                                                                                                      <  3:239886/149‑1 (MQ=255)
              cTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTgg                                                                                                                                      <  4:196347/149‑1 (MQ=255)
                 gCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAATCACTCGCCattcgt                                                                                                                                     <  2:43916/147‑6 (MQ=255)
                    gTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGc                                                                                                                                <  6:361286/149‑1 (MQ=255)
                     tGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCg                                                                                                                               <  4:61355/149‑1 (MQ=255)
                          tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATgg                                                                                                                           <  4:324108/148‑1 (MQ=255)
                          tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATg                                                                                                                            <  3:138536/147‑1 (MQ=255)
                             gcgAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAATCACTCGCCattc                                                                                                                                       <  4:309183/133‑4 (MQ=255)
                                    cGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTACACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAcc                                                                                                                <  3:41330/149‑1 (MQ=255)
                                          ggCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCACCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTc                                                                                                           <  6:273870/148‑1 (MQ=255)
                                           gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTc                                                                                                           >  7:216436/1‑147 (MQ=255)
                                                 ttCGCCATTCCTGAAACGCGCCCTATTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACaa                                                                                                   >  5:84983/1‑149 (MQ=255)
                                                 ttCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAGTGGCGAATCACTCGCCattc                                                                                                                                       >  2:340795/1‑110 (MQ=255)
                                                 ttCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAGTGGCGAATCACTCGCCattc                                                                                                                                       <  1:340795/113‑4 (MQ=255)
                                                    gCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAc                                                                                                                 <  5:130903/132‑1 (MQ=255)
                                                    gCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAc                                                                                                                 >  6:130903/1‑132 (MQ=255)
                                                       aTTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTgg                                                                                              >  8:140710/1‑148 (MQ=255)
                                                            tGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCg                                                                                        >  8:113268/1‑149 (MQ=255)
                                                            tGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCGGGAAGCCg                                                                                        >  4:291341/1‑149 (MQ=255)
                                                                     cccTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTAc                                                                                                     >  4:189066/1‑127 (MQ=255)
                                                                     cccTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTAc                                                                                                     <  3:189066/127‑1 (MQ=255)
                                                                       cTATTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTc                                                                             <  6:84983/149‑1 (MQ=255)
                                                                           tCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCa                                                                         >  3:280770/1‑149 (MQ=255)
                                                                             ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg                                                                       >  3:79864/1‑149 (MQ=255)
                                                                             ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg                                                                       >  7:146581/1‑149 (MQ=255)
                                                                             ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg                                                                       >  5:69380/1‑149 (MQ=255)
                                                                              cAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg                                                                       >  5:44148/1‑148 (MQ=255)
                                                                               aaGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGa                                                                     <  7:140710/149‑1 (MQ=255)
                                                                                   gcCTGGACGGCACGCCGTACCGCTTGCCTCCACTGCTGGAGCCCCAACGGGATCACGAATGGCGAGTGATTGGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACTGACAAc                                                                 <  3:163284/149‑1 (MQ=255)
                                                                                   gcCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAc                                                                 >  3:285795/1‑149 (MQ=255)
                                                                                    ccTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTGCCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAc                                                                 >  4:75013/1‑148 (MQ=255)
                                                                                     cTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGaa                                                                                            <  1:151256/120‑1 (MQ=255)
                                                                                     cTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGaa                                                                                            >  2:151256/1‑120 (MQ=255)
                                                                                      tGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGaa                                                                                                                       <  5:109227/92‑1 (MQ=255)
                                                                                      tGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGaa                                                                                                                       >  6:109227/1‑92 (MQ=255)
                                                                                                 cccTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGa                                                   <  4:285795/149‑1 (MQ=255)
                                                                                                    tACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCgg                                                                                       >  7:116914/1‑110 (MQ=255)
                                                                                                    tACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCgg                                                                                       <  8:116914/110‑1 (MQ=255)
                                                                                                       cGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCacta                                             >  4:78217/1‑149 (MQ=255)
                                                                                                             ccTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactact                                        <  4:79864/148‑1 (MQ=255)
                                                                                                              cTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTgg                                      <  6:44148/149‑1 (MQ=255)
                                                                                                               tCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTgg                                      >  4:23341/1‑148 (MQ=255)
                                                                                                                 cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGtt                                                                                                            >  8:297726/1‑76 (MQ=255)
                                                                                                                 cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAAccc                                                                               >  6:121501/1‑105 (MQ=255)
                                                                                                                 cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAAccc                                                                               <  5:121501/105‑1 (MQ=255)
                                                                                                                 cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTggggg                                   <  6:69380/149‑1 (MQ=255)
                                                                                                                 cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTggg                                     <  8:216436/147‑1 (MQ=255)
                                                                                                                        ggAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTg                             >  8:212654/1‑148 (MQ=255)
                                                                                                                               cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCgg                                                                                                              >  6:37884/1‑60 (MQ=255)
                                                                                                                               cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCgg                                                                                                              <  5:37884/60‑1 (MQ=255)
                                                                                                                               cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGt                     <  4:280770/149‑1 (MQ=255)
                                                                                                                                 aCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTTAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCt                   <  7:113268/149‑1 (MQ=255)
                                                                                                                                 aCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCt                   <  7:212654/149‑1 (MQ=255)
                                                                                                                                   ccGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGAGCCACTGTACGAGGTCtat                 <  3:23341/149‑1 (MQ=255)
                                                                                                                                         aCGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactac                                         <  6:57732/119‑1 (MQ=255)
                                                                                                                                         aCGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactac                                         >  5:57732/1‑119 (MQ=255)
                                                                                                                                          ccagtGGCGAATCACTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGcac           >  8:185023/5‑148 (MQ=255)
                                                                                                                                           gAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCATCGTCACGGACAACCTCGGCCGCCGTGATCAGTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACAt         >  3:112059/1‑149 (MQ=255)
                                                                                                                                               ggCGAATCACTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGa      <  7:185023/148‑1 (MQ=255)
                                                                                                                                                gCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGAc     <  3:75013/148‑1 (MQ=255)
                                                                                                                                                   aGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCt  >  6:262775/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAGCCATCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCT  >  NC_002947/4615195‑4615489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑