| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,615,345 | G→C | 9.4% | V572V (GTG→GTC) ‡ | PP_4085 → | rhs family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,615,345 | 0 | G | C | 9.4% | 195.9 / 9.4 | 64 | V572V (GTG→GTC) ‡ | PP_4085 | rhs family protein |
| Reads supporting (aligned to +/- strand): ref base G (28/30); new base C (2/4); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
TCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCT > NC_002947/4615201‑4615489 | tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTc < 4:282591/149‑1 (MQ=255)tCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAAACCGATCACGAATGGCGAGTGATTc > 1:137625/1‑149 (MQ=255) gCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTg > 3:196347/1‑149 (MQ=255) cTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTgg < 4:196347/149‑1 (MQ=255) cTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTgg < 3:239886/149‑1 (MQ=255) gCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAATCACTCGCCattcgt < 2:43916/147‑6 (MQ=255) gTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGc < 6:361286/149‑1 (MQ=255) tGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCg < 4:61355/149‑1 (MQ=255) tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATgg < 4:324108/148‑1 (MQ=255) tCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATg < 3:138536/147‑1 (MQ=255) gcgAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAATCACTCGCCattc < 4:309183/133‑4 (MQ=255) cGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTACACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAcc < 3:41330/149‑1 (MQ=255) ggCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCACCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTc < 6:273870/148‑1 (MQ=255) gcgcgcTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTc > 7:216436/1‑147 (MQ=255) ttCGCCATTCCTGAAACGCGCCCTATTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACaa > 5:84983/1‑149 (MQ=255) ttCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAGTGGCGAATCACTCGCCattc < 1:340795/113‑4 (MQ=255) ttCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAGTGGCGAATCACTCGCCattc > 2:340795/1‑110 (MQ=255) gCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAc < 5:130903/132‑1 (MQ=255) gCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTAc > 6:130903/1‑132 (MQ=255) aTTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTgg > 8:140710/1‑148 (MQ=255) tGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCg > 8:113268/1‑149 (MQ=255) tGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCGGGAAGCCg > 4:291341/1‑149 (MQ=255) cccTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTAc > 4:189066/1‑127 (MQ=255) cccTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTAc < 3:189066/127‑1 (MQ=255) cTATTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTc < 6:84983/149‑1 (MQ=255) tCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCa > 3:280770/1‑149 (MQ=255) ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg > 3:79864/1‑149 (MQ=255) ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg > 7:146581/1‑149 (MQ=255) ccAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg > 5:69380/1‑149 (MQ=255) cAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACg > 5:44148/1‑148 (MQ=255) aaGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGa < 7:140710/149‑1 (MQ=255) gcCTGGACGGCACGCCGTACCGCTTGCCTCCACTGCTGGAGCCCCAACGGGATCACGAATGGCGAGTGATTGGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACTGACAAc < 3:163284/149‑1 (MQ=255) gcCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAc > 3:285795/1‑149 (MQ=255) ccTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTGCCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAAc > 4:75013/1‑148 (MQ=255) cTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGaa > 2:151256/1‑120 (MQ=255) cTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGaa < 1:151256/120‑1 (MQ=255) tGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGaa > 6:109227/1‑92 (MQ=255) tGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGaa < 5:109227/92‑1 (MQ=255) cccTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGa < 4:285795/149‑1 (MQ=255) tACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCgg > 7:116914/1‑110 (MQ=255) tACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCgg < 8:116914/110‑1 (MQ=255) cGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCacta > 4:78217/1‑149 (MQ=255) ccTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactact < 4:79864/148‑1 (MQ=255) cTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTgg < 6:44148/149‑1 (MQ=255) tCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTgg > 4:23341/1‑148 (MQ=255) cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGtt > 8:297726/1‑76 (MQ=255) cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAAccc > 6:121501/1‑105 (MQ=255) cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAAccc < 5:121501/105‑1 (MQ=255) cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTggggg < 6:69380/149‑1 (MQ=255) cACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTggg < 8:216436/147‑1 (MQ=255) ggAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTg > 8:212654/1‑148 (MQ=255) cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCgg > 6:37884/1‑60 (MQ=255) cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCgg < 5:37884/60‑1 (MQ=255) cAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGt < 4:280770/149‑1 (MQ=255) aCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTTAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCt < 7:113268/149‑1 (MQ=255) aCCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCt < 7:212654/149‑1 (MQ=255) ccGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGAGCCACTGTACGAGGTCtat < 3:23341/149‑1 (MQ=255) aCGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactac < 6:57732/119‑1 (MQ=255) aCGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCactactac > 5:57732/1‑119 (MQ=255) ccagtGGCGAATCACTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGcac > 8:185023/5‑148 (MQ=255) gAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCATCGTCACGGACAACCTCGGCCGCCGTGATCAGTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACAt > 3:112059/1‑149 (MQ=255) ggCGAATCACTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGa < 7:185023/148‑1 (MQ=255) gCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGAc < 3:75013/148‑1 (MQ=255) aGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCt > 6:262775/1‑148 (MQ=255) | TCAACTGGCTAGCGGTGCTGTCCGCGAATACGAATGGGCGCGCTTCGCCATTCCTGAAACGCGCCCTACTCCCAAGCGCCTGGACGGCACGCCCTACCGCTTGCCTCCACTGCTGGAGCCCCAACCCGATCACGAATGGCGAGTGATTCGCCACTGGGGCAGCGATGGTGAAGAGTACCGGTTCGAGTACAACCTGGAAGCCGGTGAAACCCTCGTCACGGACAACCTCGGCCGCCGTGATCACTACTACTGGGGGCCACTGTACGAGGTCTATAAGCACATCGACCCT > NC_002947/4615201‑4615489 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |