Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,632,499	A→C	5.7%	intergenic (+104/+55)	PP_4096 → / ← PP_t59	hypothetical protein/tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,632,499	0	A	C	5.7%	220.2 / 4.1	70	intergenic (+104/+55)	PP_4096/PP_t59	hypothetical protein/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (34/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTTGAAATACCCGTTGACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGC  >  NC_002947/4632354‑4632643                                                                                                                                                  |                                                                                                                                                  tttGAAATACCCGTTGACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAgg                                                                                                                                                >  5:154879/1‑149 (MQ=255) tttGAAATACCCGTTGACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAgg                                                                                                                                                >  2:175074/1‑149 (MQ=255)          cccGTTGACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCttttt                                                                                                                                        <  6:154879/148‑1 (MQ=255)                gACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGa                                                                                                                                >  5:67851/1‑149 (MQ=255)                   gTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACtt                                                                                                                             <  6:213419/149‑1 (MQ=255)                         aGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCTTCGGGGCCtttttt                                                                                                                                       <  8:76619/134‑1 (MQ=255)                         aGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCTTCGGGGCCtttttt                                                                                                                                       >  7:76619/1‑134 (MQ=255)                           cGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACt                                                                                                                      >  2:199265/1‑148 (MQ=255)                              tatGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGa                                                                                                                  <  1:216781/149‑1 (MQ=255)                              tatGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGa                                                                                                                  <  6:221697/149‑1 (MQ=255)                                tGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGAc                                                                                                                 <  6:42389/148‑1 (MQ=255)                                   ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACg                                                                                                                                 >  2:340458/1‑129 (MQ=255)                                   ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACg                                                                                                                                 <  1:340458/129‑1 (MQ=255)                                    cAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGaaa                                                                                                            >  4:25131/1‑149 (MQ=255)                                     aGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAAc                                                                                                           <  5:141509/149‑1 (MQ=255)                                       gTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGa                                                                                                                                <  7:147930/126‑1 (MQ=255)                                       gTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGa                                                                                                                                <  5:265483/126‑1 (MQ=255)                                       gTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGa                                                                                                                                >  8:147930/1‑126 (MQ=255)                                       gTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGa                                                                                                                                >  6:265483/1‑126 (MQ=255)                                           cccACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTg                                                                                                        >  1:98782/1‑146 (MQ=255)                                            ccACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGa                                                                                                                  <  8:30803/135‑1 (MQ=255)                                            ccACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGa                                                                                                                  >  7:30803/1‑135 (MQ=255)                                            ccACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAAc                                                                                                           <  6:198314/142‑1 (MQ=255)                                            ccACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAAc                                                                                                           >  5:198314/1‑142 (MQ=255)                                              aCGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATc                                                                                                  >  6:188062/1‑149 (MQ=255)                                                 gCGTAACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCttt                                                                                               <  3:205920/149‑1 (MQ=255)                                                      aCGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTg                                                                                          <  6:67851/149‑1 (MQ=255)                                                        ggCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGa                                                                                        <  2:98782/149‑1 (MQ=255)                                                          ccAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGa                                                                                                                  <  8:38653/121‑1 (MQ=255)                                                            aGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCgg                                                                                    >  5:69769/1‑149 (MQ=255)                                                            aGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCgg                                                                                    <  1:175074/149‑1 (MQ=255)                                                            aGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCgg                                                                                    >  5:64627/1‑149 (MQ=255)                                                             gggCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCggg                                                                                   <  5:188062/149‑1 (MQ=255)                                                                         gtggtgTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑a                                                                                                                                    >  3:169781/1‑88 (MQ=255)                                                                         gtggtgTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑a                                                                                                                                    <  4:169781/88‑1 (MQ=255)                                                                            gtgtTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGaa                                                                     <  1:199265/148‑1 (MQ=255)                                                                            gtgtTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAAc                                                                    >  7:254996/1‑149 (MQ=255)                                                                                   tACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGa                                                              <  6:69769/148‑1 (MQ=255)                                                                                   tACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGa                                                              <  6:64627/148‑1 (MQ=255)                                                                                     cTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGt                                                                                                       >  7:298563/1‑105 (MQ=255)                                                                                     cTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGt                                                                                                       <  8:298563/105‑1 (MQ=255)                                                                                      ttGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTAttt                                                                                           >  6:99151/1‑116 (MQ=255)                                                                                      ttGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTAttt                                                                                           <  5:99151/116‑1 (MQ=255)                                                                                      ttGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGAcccc                                                          >  5:146711/1‑149 (MQ=255)                                                                                           tttCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTc                                                                        <  6:74263/130‑1 (MQ=255)                                                                                           tttCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTc                                                                        >  5:74263/1‑130 (MQ=255)                                                                                           tttCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCt                                                     >  1:107644/1‑149 (MQ=255)                                                                                                tAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGAc                                                       <  4:197761/142‑1 (MQ=255)                                                                                                tAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGAc                                                       >  3:197761/1‑142 (MQ=255)                                                                                                  gagGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCAAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                              >  4:286941/1‑149 (MQ=255)                                                                                                             aTTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                        >  2:26384/1‑144 (MQ=255)                                                                                                             aTTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                        <  1:26384/144‑1 (MQ=255)                                                                                                              ttGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                  <  2:107644/149‑1 (MQ=255)                                                                                                                       aaTGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                         >  5:265223/1‑149 (MQ=255)                                                                                                                            aTTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                    >  7:162119/1‑149 (MQ=255)                                                                                                                             ttCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCTAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                    >  1:53067/1‑149 (MQ=255)                                                                                                                             ttCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                   >  4:277390/1‑149 (MQ=255)                                                                                                                             ttCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                   >  7:272782/1‑149 (MQ=255)                                                                                                                               cgaacgaaAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcg                 >  6:341346/1‑149 (MQ=255)                                                                                                                                gaacgaaAAAAGGCCCCGAAGGGCCTTTTTTC‑AGCGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCaa                                               >  1:133968/1‑118 (MQ=255)                                                                                                                                gaacgaaAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCaa                                               <  2:133968/118‑1 (MQ=255)                                                                                                                                 aacgaaAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                              <  1:126140/118‑1 (MQ=255)                                                                                                                                 aacgaaAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                              >  2:126140/1‑118 (MQ=255)                                                                                                                                    gaaAAAAGGCCCTTCGGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                              >  3:218199/1‑115 (MQ=25)                                                                                                                                    gaaAAAAGGCCCTTCGGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAg                                              <  4:218199/115‑1 (MQ=25)                                                                                                                                          aGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg      <  6:146711/149‑1 (MQ=255)                                                                                                                                           ggCCCCTAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATggg     <  3:286941/149‑1 (MQ=255)                                                                                                                                           ggCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTcgc                                                                <  3:99689/90‑1 (MQ=255)                                                                                                                                           ggCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTcgc                                                                >  4:99689/1‑90 (MQ=255)                                                                                                                                              cccGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGc  >  3:176856/1‑149 (MQ=255)                                                                                                                                                  aaGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGAccc                                                           >  6:187843/1‑88 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TTTGAAATACCCGTTGACGTGATCAGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTC‑AACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGC  >  NC_002947/4632354‑4632643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑