Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,694,425 C→G 8.3% P767R (CCC→CGC)  PP_4154 → class 3 aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,694,4250CG 8.3% 170.9 / 2.2 57P767R (CCC→CGC) PP_4154class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base G (2/3);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAC  >  NC_002947/4694289‑4694569
                                                                                                                                        |                                                                                                                                                
ttCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAggtagcct                                                                                                                                      >  2:345963/1‑143 (MQ=255)
           cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAGcga                                                                                                                           <  1:345963/149‑3 (MQ=255)
            gACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAg                                                                                                                                             <  8:11857/130‑1 (MQ=255)
            gACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAg                                                                                                                                             >  7:11857/1‑130 (MQ=255)
                 cgccgcCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGcc                                                                                                                      >  2:342290/1‑148 (MQ=255)
                  gccgccGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTg                                                                                                                                     >  6:354346/1‑132 (MQ=255)
                  gccgccGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTg                                                                                                                                     <  5:354346/132‑1 (MQ=255)
                              cTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                             >  8:88543/1‑128 (MQ=255)
                              cTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                             <  7:88543/128‑1 (MQ=255)
                               tGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             >  6:280042/1‑143 (MQ=255)
                               tGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             <  5:280042/143‑1 (MQ=255)
                                gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             >  5:101106/1‑142 (MQ=255)
                                gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             <  6:101106/142‑1 (MQ=255)
                                  aaGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGc                                                                                                                                           >  1:9552/1‑110 (MQ=255)
                                  aaGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGc                                                                                                                                           <  2:9552/110‑1 (MQ=255)
                                       gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                               >  3:46213/1‑149 (MQ=255)
                                         cGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtgt                                                                                             <  1:342290/149‑1 (MQ=255)
                                          gACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtgt                                                                                             <  2:278614/148‑1 (MQ=255)
                                                      gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAGCCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                             <  5:266600/104‑1 (MQ=255)
                                                      gctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAGCCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGc                                                                                                                             >  6:266600/1‑104 (MQ=255)
                                                           ccTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGc                                                                           <  4:46213/149‑1 (MQ=255)
                                                             tCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGt                                                                                                                 >  6:206696/1‑109 (MQ=255)
                                                              cGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAgg                                                                        >  2:272364/1‑149 (MQ=255)
                                                               gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGc                                                                                           >  6:79238/1‑129 (MQ=255)
                                                               gACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGc                                                                                           <  5:79238/129‑1 (MQ=255)
                                                                aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCTCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtg                                                                                              <  1:71545/125‑1 (MQ=255)
                                                                aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtg                                                                                              >  2:71545/1‑125 (MQ=255)
                                                                aCGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTCTACGGCAATGCCGGCTGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCTCCGACGAAGAGCAgg                                                                        >  6:213378/1‑147 (MQ=255)
                                                                  gCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAgg                                                                    >  5:251973/1‑149 (MQ=255)
                                                                    ccGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             <  3:244761/106‑1 (MQ=255)
                                                                    ccGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCcgc                                                                                                             >  4:244761/1‑106 (MQ=255)
                                                                       tGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATg                                                               <  7:94777/149‑1 (MQ=255)
                                                                       tGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATg                                                               <  8:67699/149‑1 (MQ=255)
                                                                       tGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATg                                                               <  1:170228/149‑1 (MQ=255)
                                                                                     cGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAg                                                 <  1:272364/149‑1 (MQ=255)
                                                                                        gCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTAc                                              <  2:79181/149‑1 (MQ=255)
                                                                                         catcatCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTa                                               >  8:251179/1‑147 (MQ=255)
                                                                                         catcatCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTa                                               <  7:251179/147‑1 (MQ=255)
                                                                                             atcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                            <  1:4846/98‑1 (MQ=255)
                                                                                             atcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                            >  2:4846/1‑98 (MQ=255)
                                                                                             atcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTg                                         >  7:121359/1‑149 (MQ=255)
                                                                                             atcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTg                                         >  7:305013/1‑149 (MQ=255)
                                                                                             atcTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTg                                         >  1:117728/1‑149 (MQ=255)
                                                                                                tGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGc                                                                                           <  3:261200/96‑1 (MQ=255)
                                                                                                tGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGc                                                                                           >  4:261200/1‑96 (MQ=255)
                                                                                                tGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgggg                                      >  1:38735/1‑149 (MQ=255)
                                                                                                  cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGa                                                  <  2:241860/135‑1 (MQ=255)
                                                                                                  cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                        <  8:121359/145‑1 (MQ=255)
                                                                                                  cGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAAGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGa                                                  >  1:241860/1‑135 (MQ=255)
                                                                                                                        ggcggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCACCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGcc              >  1:39809/1‑149 (MQ=255)
                                                                                                                          cggTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCgg            <  2:38735/149‑1 (MQ=255)
                                                                                                                             tATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAca         <  6:251973/149‑1 (MQ=255)
                                                                                                                                 tcGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAcatcat     >  6:8716/1‑149 (MQ=255)
                                                                                                                                   gCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCACCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAcatc       <  2:39809/145‑1 (MQ=255)
                                                                                                                                    cTGCGCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc  <  5:213378/149‑1 (MQ=255)
                                                                                                                                     tGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtg                   >  7:155733/1‑131 (MQ=255)
                                                                                                                                     tGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtg                   <  8:155733/131‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                
TTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAC  >  NC_002947/4694289‑4694569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑