| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 447,152 | T→G | 6.8% | intergenic (+32/‑270) | PP_0368 → / → PP_0369 | acyl‑CoA dehydrogenase/diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 447,152 | 0 | T | G | 6.8% | 183.9 / 5.0 | 59 | intergenic (+32/‑270) | PP_0368/PP_0369 | acyl‑CoA dehydrogenase/diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (30/25); new base G (2/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
CTTCCAGCGCATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAACCCGCTCC > NC_002947/447015‑447300 | cTTCCAGCGCATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGc > 7:92506/1‑149 (MQ=255) cAGCGCATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCAAAAACCCGCCT‑CCCGGTCAGGGCAGGCGGGt < 3:40/149‑1 (MQ=255) cATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt > 7:42324/1‑148 (MQ=255) cATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt > 6:79156/1‑148 (MQ=255) cTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCgg < 4:54231/138‑1 (MQ=255) cTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCgg > 3:54231/1‑138 (MQ=255) cTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACg < 8:42324/148‑1 (MQ=255) tGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTc > 4:159480/1‑149 (MQ=255) cccgtacccATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt < 2:235846/134‑1 (MQ=255) cccgtacccATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt > 1:235846/1‑134 (MQ=255) ccgtacccATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCa < 5:79156/149‑1 (MQ=255) tacccATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt < 6:53853/130‑1 (MQ=255) tacccATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGttttt > 5:53853/1‑130 (MQ=255) gACCATGCTGTGGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCg < 5:222754/147‑1 (MQ=255) gACCATGCTGTAGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCg > 6:222754/1‑147 (MQ=255) aCCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAg < 5:322088/149‑1 (MQ=255) aCCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAg < 1:209332/149‑1 (MQ=255) cATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGa < 8:92506/149‑1 (MQ=255) gCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCgg > 7:292808/1‑106 (MQ=255) gCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCgg < 8:292808/106‑1 (MQ=255) cTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCgg < 5:33506/149‑1 (MQ=255) tCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCggg < 2:322497/147‑1 (MQ=255) gggCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTa > 3:226173/1‑149 (MQ=255) ggCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGt > 6:188945/1‑128 (MQ=255) ggCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGt < 5:188945/128‑1 (MQ=255) caacaaCCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCcgc > 1:271837/1‑148 (MQ=255) gATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCctg > 3:222586/1‑149 (MQ=255) ggACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAaga > 4:64006/1‑135 (MQ=255) ggACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAaga < 3:64006/135‑1 (MQ=255) gACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTgg < 4:222586/149‑1 (MQ=255) gAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCa < 4:226173/149‑1 (MQ=255) ttCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCAt > 3:156544/1‑148 (MQ=255) tACTGATACTGGCGCTGTACCCATAACCCGCTTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCAcc < 6:250592/148‑1 (MQ=255) aCTGATACTGGCGCTGTACCCAAAACCCGCCAGCGCTGACCGAG‑AGGCGGGTTTTATACGTCTTCAGGTTCAGCGTGGACCCTCGGAGGAGCGGGGTTAACCGCGAAGAGTCCGGCCCTCCTGGACAATCTCAATAATCCCTTCACCg > 5:250592/1‑148 (MQ=255) tGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAggccg < 6:91159/112‑1 (MQ=255) tGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAggccg > 5:91159/1‑112 (MQ=255) aTACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGTCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTga > 3:240109/1‑147 (MQ=255) gcTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCCTCAGCCGTTCACCGTTGCCATCGCTAc > 4:272876/1‑149 (MQ=255) cccAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCAcc > 1:22348/1‑129 (MQ=255) cccAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCAcc < 2:22348/129‑1 (MQ=255) cccAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTAtt < 4:156544/149‑1 (MQ=255) cccAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTAt > 1:340234/1‑148 (MQ=255) cAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTcg > 2:80796/1‑149 (MQ=255) aaaaCCCGCCT‑CCCGGTCAGGGCAGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCAACAGCCGTTCACCGTTGCCAGCGCTACCGTATTcgc > 2:23538/1‑149 (MQ=25) aaaCCCGCCT‑CCCGGTCAGGGCAGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCa < 5:286985/132‑1 (MQ=25) aaaCCCGCCT‑CCCGGTCAGGGCAGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCa > 6:286985/1‑132 (MQ=25) cccGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCggg > 3:96305/1‑148 (MQ=255) ccGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCctgc > 8:241084/1‑95 (MQ=255) ccGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCctgc < 7:241084/95‑1 (MQ=255) ccGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGt > 4:56154/1‑148 (MQ=255) ccGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGt > 1:268304/1‑148 (MQ=255) ccTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAAc < 4:96305/149‑1 (MQ=255) cTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAAcc < 7:32636/149‑1 (MQ=255) cTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAAcc > 2:84937/1‑149 (MQ=255) cccTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTg < 2:269929/118‑1 (MQ=255) cccTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTg > 5:101892/1‑118 (MQ=255) cccTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTg > 1:269929/1‑118 (MQ=255) cccTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTg < 6:101892/118‑1 (MQ=255) cTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAACCCGCTc > 4:154670/1‑149 (MQ=255) tGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAACCCGCTcc > 4:74244/1‑149 (MQ=255) | CTTCCAGCGCATCCTGCCACGTACCCGTACCCATGTGGCGACCATGCTGTCGGGCGCCAACAACCTGATGGCGATGGACGAAGAGCACTTCGGCCTGTCGTACTGATACTGGCGCTGTACCCAAAACCCGCCTGCCCTGACCGGG‑AGGCGGGTTTTTTACGTCTTCAGGTTCAGCGTGGTCCCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCTGCTGGCCAATCTCCATCAGCCGTTCACCGTTGCCAGCGCTACCGTATTCGCGGGTAAACCCGCTCC > NC_002947/447015‑447300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |