Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,627 C→T 14.2% intergenic (‑185/+33) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,6270CT14.2% 99.2 / 6.3 47intergenic (‑185/+33)pvdH/PP_4224diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (18/19);  new base T (4/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGC  >  NC_002947/4796480‑4796767
                                                                                                                                                     |                                                                                                                                            
gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTc                                                                                                                                              <  5:266452/148‑1 (MQ=255)
   aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                                          <  5:266095/149‑1 (MQ=255)
     gggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc                                                                                                                                        >  6:233662/1‑149 (MQ=255)
      ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc                                                                                                                                        >  5:206967/1‑148 (MQ=255)
       gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg                                                                                                                                       >  7:22193/1‑148 (MQ=255)
        tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGaa                                                                                                                                     <  6:219648/149‑1 (MQ=37)
        tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg                                                                                                                                       >  4:106243/1‑147 (MQ=255)
         aaaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa                                                                                                                                     <  3:106243/148‑1 (MQ=255)
           aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAagag                                                                                                                                  <  4:55163/149‑1 (MQ=255)
                      cAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                                       <  8:22193/149‑1 (MQ=255)
                         gCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                                          <  8:245433/127‑1 (MQ=255)
                         gCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                                          >  7:245433/1‑127 (MQ=255)
                                                gAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGAcgcg                                                                                             >  5:115301/1‑149 (MQ=255)
                                                  aaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc                                                                                            <  5:233662/148‑1 (MQ=255)
                                                   aCGCTTGTGTGGGGGGAGGATTTAGCGGTTGTTAGGCTTTTTTATTTTTGTCTTTGCTGGCCTCTTCGCGGGTAAACCGGCCGGTAGTCGGGGTTTAACCCGGGATTTGGCAAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCg                                                                                          <  6:115301/149‑1 (MQ=255)
                                                   aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCCGGTTAAACCCCCCCTTAGAGCGGGTTGAACCGCCCAGAGGGCCGCACCCGGGCAAAACAACAAATGCCACGACgcgcgg                                                                                          >  6:224940/1‑147 (MQ=255)
                                                                  gaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAg                                                                                                   <  3:242616/125‑1 (MQ=255)
                                                                  gaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAg                                                                                                   >  4:242616/1‑125 (MQ=255)
                                                                    gtaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTCCTGGCCTCTTCGCGGGTAAACCCGCCGTTTGCAGCGGGTTCACCCGCTAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcc                                                                         <  5:224940/147‑1 (MQ=255)
                                                                     gaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTAGCGGGTTAAACCCCCCCGAGGAGCGGGGTGACCCGCGAAGAGGGCACCACACGCGATAAAAAAAAAAGCCCCAGACGCGCCGGGAACAGCAACAAAAacc                                                                        >  2:161587/1‑149 (MQ=255)
                                                                              gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGt                                                               >  6:334429/1‑149 (MQ=255)
                                                                                    aCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGGACGAGCGGGTTAACCCGCGAAGAGGCCCGCACAGGCGAAAAAACTCAATGCCCAGGCCGCGCCGGTACCTGCCACAAAACCCCCAAGCCGTCACAAc                                                         >  4:203850/1‑149 (MQ=25)
                                                                                        gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGccc                                                                                                     >  3:69547/1‑101 (MQ=255)
                                                                                        gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGccc                                                                                                     <  4:69547/101‑1 (MQ=255)
                                                                                               tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGTAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATACACATCAATGCCCa                                                                                                    <  4:51163/95‑1 (MQ=255)
                                                                                               tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATACACATCAATGCCCa                                                                                                    >  3:51163/1‑95 (MQ=255)
                                                                                                   tGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgcgc                                           <  1:161587/148‑1 (MQ=255)
                                                                                                    gtgtgtgGTGCCCTCTGCGCGGTAAAACCCCCCCTTCGCCGGGGTTTCACCGGCAAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCa                                          <  6:59862/145‑1 (MQ=255)
                                                                                                            tGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCt                                      <  8:2564/144‑1 (MQ=255)
                                                                                                            tGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCt                                      >  7:2564/1‑144 (MQ=255)
                                                                                                                ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTa                                                          >  1:312944/1‑120 (MQ=255)
                                                                                                                ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTa                                                          <  2:312944/120‑1 (MQ=255)
                                                                                                                ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGcaca                             >  1:107849/1‑149 (MQ=255)
                                                                                                                      gcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCt                        <  2:107849/148‑1 (MQ=255)
                                                                                                                         gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa                     >  2:336835/1‑149 (MQ=38)
                                                                                                                          ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                    >  4:331601/1‑149 (MQ=38)
                                                                                                                          ggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                    >  7:171205/1‑149 (MQ=255)
                                                                                                                             tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTCCGGCTGTCAAAGCGCAGGCTACCGGCACACCCATGCACGa                  >  7:30757/3‑149 (MQ=37)
                                                                                                                               aaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                    >  5:98754/1‑145 (MQ=38)
                                                                                                                               aaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                    <  6:98754/145‑1 (MQ=38)
                                                                                                                               aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc                <  5:334429/149‑1 (MQ=255)
                                                                                                                                   cgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt            <  3:203850/149‑1 (MQ=255)
                                                                                                                                          aGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg                                         <  3:100102/113‑1 (MQ=255)
                                                                                                                                          aGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg                                         >  4:100102/1‑113 (MQ=255)
                                                                                                                                               cGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGGACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGc  >  5:184421/1‑147 (MQ=255)
                                                                                                                                               cGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATAACTGCCCAGGACGCGCCGGTACCTGAAACAACCCCCGCAAGCCGGCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCCCAATCGc  >  5:179285/1‑147 (MQ=255)
                                                                                                                                                  gTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg                                 <  6:71849/113‑1 (MQ=255)
                                                                                                                                                  gTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg                                 >  5:71849/1‑113 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGC  >  NC_002947/4796480‑4796767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: