Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,803,182	C→G	8.7%	V44V (GTG→GTC) ‡	PP_4231 ←	xanthine dehydrogenase accessory factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,803,182	0	C	G	8.7%	208.3 / 8.9	69	V44V (GTG→GTC) ‡	PP_4231	xanthine dehydrogenase accessory factor
Reads supporting (aligned to +/- strand):  ref base C (28/35);  new base G (2/4);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GGCGAACGCGCGAACGCGGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCACGCTTTA  >  NC_002947/4803037‑4803319                                                                                                                                                      |                                                                                                                                            ggCGAACGCGCGAACGCGGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCAcc                                                                                                                                          >  2:218980/1‑149 (MQ=255)    gaacgcgcgaacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGcc                                                                                                                                       >  3:188506/1‑149 (MQ=255)        gcgcgaacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCcagca                                                                                                                                   <  8:313521/149‑1 (MQ=255)          gcgaacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGCATCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCcagcag                                                                                                                                  >  1:252079/1‑149 (MQ=255)          gcgaacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCTGCTGGCGGTGAACGCCagcggc                                                                                                                                 <  3:93245/149‑1 (MQ=255)            gaacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCTGCTGGCGGTGAACGCCagcggcca                                                                                                                               <  2:241089/149‑3 (MQ=255)             aacgcgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGaa                                                                                                                              <  4:7696/149‑1 (MQ=255)                 cgGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCg                                                                                                                          >  7:220123/1‑149 (MQ=255)                  gggTCG‑TGCCCTCGCCAAAACCCCACG‑TGGCAACCGGC‑TCCGGCAATTCCCCGAGGGCCACGCGGTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGgc                                                                                                                        <  7:313155/149‑1 (MQ=255)                   ggTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGgc                                                                                                                        <  1:45093/149‑1 (MQ=255)                          gCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCAcgcg                                                                                                                 <  7:263207/149‑1 (MQ=255)                                gCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCg                                                                                                          <  8:220123/149‑1 (MQ=255)                                  cATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCggg                                                                                                        <  2:80429/149‑3 (MQ=255)                                   aTAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCgggt                                                                                                       >  4:206188/1‑146 (MQ=255)                                     aa‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCgggttc                                                                                                     <  3:206188/149‑6 (MQ=255)                                      a‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCgggttcg                                                                                                    <  7:283905/149‑7 (MQ=255)                                         gCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAg                                                                                                  >  8:144815/1‑149 (MQ=255)                                         gCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAg                                                                                                  >  8:133442/1‑149 (MQ=255)                                         gCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAg                                                                                                  <  4:188506/149‑1 (MQ=255)                                          cACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAgg                                                                                                 <  2:252079/149‑1 (MQ=255)                                           aCGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGt                                                                                                >  3:36311/1‑149 (MQ=255)                                              aTGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGc                                                                                             <  8:333858/149‑1 (MQ=255)                                                    aCCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCgccgcg                                                                                       <  4:36311/149‑6 (MQ=255)                                                       ggC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACtgt                                                                                    <  2:147738/149‑1 (MQ=255)                                                             cgtgAATTCCCCGAGGGCCACGCGCTCGAGAAAGTCGTCTTCCCCAGACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACtgtg                                                                                   <  1:289728/144‑1 (MQ=255)                                                             cGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGcac                                                                                <  7:133442/148‑1 (MQ=255)                                                               ggAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAgg                                                                                                 <  4:140364/129‑1 (MQ=255)                                                               ggAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAgg                                                                                                 >  3:140364/1‑129 (MQ=255)                                                                   ttCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATa                                                                         >  5:128348/1‑149 (MQ=255)                                                                   ttCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑CCGCCGAGCCGTAGGTCGCGAGCACTGTGCACAACCATa                                                                         >  7:196839/1‑149 (MQ=255)                                                                     ccccGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGCCAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGCGCCGTAGGTGGCGAGCACTGTGCACAGCCaga                                                                         >  6:340158/1‑145 (MQ=255)                                                                     ccccGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGc                                                                                                       <  5:121564/117‑1 (MQ=255)                                                                     ccccGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATa                                                                         <  5:340158/147‑1 (MQ=255)                                                                      cccGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑ccc                                                                     >  7:232471/1‑149 (MQ=255)                                                                         gAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGa                                                                                           >  4:249094/1‑125 (MQ=255)                                                                                  gcgTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGtt                                                           >  1:65474/1‑147 (MQ=255)                                                                                  gcgTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGtt                                                           <  2:65474/147‑1 (MQ=255)                                                                                    gTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAg                                                       <  8:281680/149‑1 (MQ=255)                                                                                     ttCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCgggttc                                                                                                     <  4:284744/103‑6 (MQ=255)                                                                                     ttCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGC‑CGCGTGCGCCgggttc                                                                                                     >  3:284744/1‑98 (MQ=255)                                                                                         agaAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCa                                                  <  5:318247/149‑1 (MQ=255)                                                                                         agaAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCa                                                  >  1:195589/1‑149 (MQ=255)                                                                                             aGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTg                                               >  2:269785/1‑148 (MQ=255)                                                                                              gtcgtcTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCa                                             <  2:195589/149‑1 (MQ=255)                                                                                                cgtcTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTc                                                                 >  5:164144/1‑127 (MQ=255)                                                                                                cgtcTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTc                                                                 <  6:164144/127‑1 (MQ=255)                                                                                                    tt‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAg                                                       <  6:90750/133‑1 (MQ=255)                                                                                                    tt‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAg                                                       >  5:90750/1‑133 (MQ=255)                                                                                                        cGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCt                                       <  8:232471/147‑1 (MQ=255)                                                                                                        cGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGa                                     >  6:111397/1‑149 (MQ=255)                                                                                                         gACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATACCCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGa                                     <  1:52460/149‑1 (MQ=255)                                                                                                         gACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGa                                     >  2:52460/1‑148 (MQ=255)                                                                                                          acacAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGc                                              >  5:27565/1‑138 (MQ=255)                                                                                                          acacAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGc                                              <  6:27565/138‑1 (MQ=255)                                                                                                              aGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGAt                                >  3:266967/1‑148 (MQ=255)                                                                                                                ccACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCAc                             <  1:269785/149‑1 (MQ=255)                                                                                                                   ccGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTg                          <  4:266967/149‑1 (MQ=255)                                                                                                                           cGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGc                                                                                  >  7:22597/1‑86 (MQ=255)                                                                                                                           cGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGc                                                                                  <  8:22597/86‑1 (MQ=255)                                                                                                                                     cACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCAc        >  1:306046/1‑149 (MQ=255)                                                                                                                                      aCTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCgag                 <  6:240615/139‑1 (MQ=255)                                                                                                                                      aCTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCgag                 >  5:240615/1‑139 (MQ=255)                                                                                                                                      aCTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCa         <  1:276739/147‑1 (MQ=255)                                                                                                                                      aCTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCa         >  2:276739/1‑147 (MQ=255)                                                                                                                                           ctgCTGGCGGTGAACGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCACGCtt    >  5:22318/3‑147 (MQ=255)                                                                                                                                           ctgCTGGCGGTGAACGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCACGCtt    <  6:22318/145‑1 (MQ=255)                                                                                                                                           ccGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCACGCTTTa  <  2:306046/149‑1 (MQ=255)                                                                                                                                            tgCTGGCGGTGAACGCCAGCGGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTg                          >  7:86835/2‑124 (MQ=255)                                                                                                                                            tgCTGGCGGTGAACGCCAGCGGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTg                          <  8:86835/123‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                            GGCGAACGCGCGAACGCGGGTCGCTGCCGTCGCCATAA‑CGCACGATGGCAACCGGC‑TCCGGGAATTCCCCGAGGGCCACGCGTTCGAGAAAGTCGTCTT‑‑CGACACAGCCACCGGACAGCGAGCCCAGCCACTGGCCGCTGGCGTTCACCGCCAGCAGCGAACCCGGCGCACGCG‑GCGCCGAGCCGTAGGTGGCGAGCACTGTGCACAACCATA‑CCCGCTCGCCGTTGCAGGACCACTGCAAAGCCTGACGGATCACCTGTAGATCGAGATGCTGCACGCTTTA  >  NC_002947/4803037‑4803319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑