Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 86,245 A→C 7.8% M158R (ATG→AGG)  PP_0075 ← choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294786,2450AC 7.8% 153.2 / 4.0 51M158R (ATG→AGG) PP_0075choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base C (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGT  >  NC_002947/86101‑86393
                                                                                                                                                |                                                                                                                                                     
ggCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGcc                                                                                                                                                   >  1:176019/1‑149 (MQ=255)
       gTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCATGGCCCTGCCcag‑ccag                                                                                                                                           <  7:141572/149‑4 (MQ=255)
                    tttGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑AAGGTTGGGTAGCTGGc                                                                                                                              >  5:362248/1‑149 (MQ=255)
                       gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGtt                                                                                                                                         >  7:8689/1‑135 (MQ=255)
                       gAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGtt                                                                                                                                         <  8:8689/135‑1 (MQ=255)
                         aGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAg                                                                                                                         <  6:92284/149‑1 (MQ=255)
                          ggCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAgg                                                                                                                        <  2:176019/149‑1 (MQ=255)
                            acGCACCGATCACGACGGTGGCCAGGCCAAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGTGCCATGCCCAG‑CAGGTTGGTTAGCTGGCCCAAGGCa                                                                                                                      <  2:242315/148‑1 (MQ=255)
                                 ccGATCACGACGGGGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGAc                                                                                                                 <  7:194490/149‑1 (MQ=255)
                                         gacgGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                                         >  8:111051/1‑149 (MQ=255)
                                                       ccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                                  <  6:21602/126‑1 (MQ=255)
                                                       ccAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGa                                                                                                                  >  5:21602/1‑126 (MQ=255)
                                                           cagcaAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGa                                                                                       <  7:111051/149‑1 (MQ=255)
                                                                 ggaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGaa                                                                                            <  2:3517/138‑1 (MQ=255)
                                                                 ggaaggTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGaa                                                                                            >  1:3517/1‑138 (MQ=255)
                                                                      gtaggtCGACCTCGCCTTGGTGGCTGGCCAGGTCCTGCAGGGTCATCAGCGCGGTTGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCGCAAGGCAATGACGATGGCGGCACCCAg                                                                                                  <  2:106416/127‑1 (MQ=255)
                                                                      gtaggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAg                                                                                                  >  1:106416/1‑127 (MQ=255)
                                                                        aggtCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATg                                                                          >  2:234209/1‑149 (MQ=255)
                                                                                   gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGt                                                               >  7:143535/1‑149 (MQ=255)
                                                                                   gCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGt                                                               >  6:221577/1‑149 (MQ=255)
                                                                                     cTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                              >  4:164685/1‑148 (MQ=255)
                                                                                       gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                                         >  5:322796/1‑103 (MQ=255)
                                                                                       gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATggcgg                                                                                                         <  6:322796/103‑1 (MQ=255)
                                                                                       gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACg                                                                               >  5:331044/1‑129 (MQ=255)
                                                                                       gCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACg                                                                               <  6:331044/129‑1 (MQ=255)
                                                                                            gctggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgcg                                                      >  7:190352/1‑149 (MQ=255)
                                                                                               ggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAg                                                                                           <  7:311981/109‑1 (MQ=255)
                                                                                               ggcCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAg                                                                                           >  8:311981/1‑109 (MQ=255)
                                                                                                      tCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAgcg                                            <  1:234209/149‑1 (MQ=255)
                                                                                                           cACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                                         >  6:132044/1‑147 (MQ=255)
                                                                                                           cACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCaa                                         >  8:249168/1‑147 (MQ=255)
                                                                                                             cGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGc                                     <  8:143535/149‑1 (MQ=255)
                                                                                                               gTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                              <  5:192716/122‑1 (MQ=255)
                                                                                                               gTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGtt                                                              >  6:192716/1‑122 (MQ=255)
                                                                                                                  atcaGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGag                                >  8:114547/1‑149 (MQ=255)
                                                                                                                      gcgcGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagc                            <  6:327005/149‑1 (MQ=255)
                                                                                                                        gcgGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcagcag                          <  7:192805/149‑1 (MQ=255)
                                                                                                                             caacctgctggGCATGGCCCTGCCCAG‑CCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcag                             >  2:133494/7‑141 (MQ=255)
                                                                                                                             caacctgctggGCATGGCCCTGCCCAG‑CCGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagcag                             <  1:133494/135‑1 (MQ=255)
                                                                                                                                ctggctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGaaaa                  >  3:274834/1‑149 (MQ=255)
                                                                                                                                  tgctggGCATGGCCCTGCCCAGCCAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAAt                 >  6:66732/2‑149 (MQ=255)
                                                                                                                                   gctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGATAAt                 <  8:82541/147‑1 (MQ=255)
                                                                                                                                   gctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAAt                 <  8:190352/147‑1 (MQ=255)
                                                                                                                                    ctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagc                               <  4:233316/132‑1 (MQ=255)
                                                                                                                                    ctggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGagc                               >  3:233316/1‑132 (MQ=255)
                                                                                                                                     tggGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATgccg             >  1:115261/1‑149 (MQ=255)
                                                                                                                                            ggCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGCCCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAgg       >  7:247873/1‑148 (MQ=255)
                                                                                                                                            ggCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgcgcgc                                                   >  6:16229/1‑104 (MQ=255)
                                                                                                                                            ggCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAgcgcgc                                                   <  5:16229/104‑1 (MQ=255)
                                                                                                                                             gCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAgg       >  2:16556/1‑147 (MQ=255)
                                                                                                                                              ccATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAg    <  3:164685/149‑1 (MQ=255)
                                                                                                                                                aTGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                               >  6:187158/1‑104 (MQ=255)
                                                                                                                                                aTGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGaa                                               <  5:187158/104‑1 (MQ=255)
                                                                                                                                                aTGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGt  <  7:249168/149‑1 (MQ=255)
                                                                                                                                                aTGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGt  <  5:221577/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GGCCAGCGTGGGCGCCAGAGTTTGAAGGCCGCACCGATCACGACGGTGGCCAGGCCCAGCAGCAAGGAAGGTAGGTCGACCTCGCCTGCGTGGCTGGCCAGGTCCTGCACGGTCATCAGCGCGGTGGCCTGGCTGGGCAGGGCCATGCCCAG‑CAGGTTGGGTAGCTGGCCCAAGGCAATGACGATGGCGGCACCCAGCGTGAAGCCGAGCACCACGGAATGGGAGACGAAGTTGACCAGCGCGCCGAAGCGCAATAGCCCGAGCAGCAGCTGGAAAATGCCGCCGAGGAAGGT  >  NC_002947/86101‑86393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑