Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,895,897 G→A 12.2% V118I (GTC→ATC) ‡ PP_4304 → VIC family cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,895,8970GA12.2% 124.8 / 6.8 49V118I (GTC→ATC) ‡PP_4304VIC family cation transporter
Reads supporting (aligned to +/- strand):  ref base G (19/24);  new base A (2/4);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CTGGCCGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTC  >  NC_002947/4895750‑4896040
                                                                                                                                                   |                                                                                                                                               
cTGGCCGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCggg                                                                                                                                                 <  2:304763/148‑1 (MQ=255)
      gAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCg                                                                                                                                          >  3:196361/1‑149 (MQ=255)
           cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgc                                                                                                                                     <  1:287943/149‑1 (MQ=255)
           cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga                                                                                                                                     <  3:213153/149‑2 (MQ=255)
            aTCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtgat                                                                                                                                    <  7:133659/149‑3 (MQ=255)
               aCCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctgc                                                                                                                                  <  7:38967/148‑1 (MQ=255)
                 ccGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctg                                                                                                                                   >  5:86011/1‑145 (MQ=255)
                   gCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga                                                                                                                                  <  4:292243/144‑2 (MQ=255)
                   gCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga                                                                                                                                  >  3:292243/1‑143 (MQ=255)
                        tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc                                                                                                                         >  3:99481/1‑148 (MQ=255)
                        tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc                                                                                                                         <  4:99481/148‑1 (MQ=255)
                          cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                       <  3:332316/148‑1 (MQ=255)
                           tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatgag                                                                                                                                 <  7:209549/137‑3 (MQ=255)
                           tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatgag                                                                                                                                 >  8:209549/1‑135 (MQ=255)
                            gTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                       >  7:38783/1‑146 (MQ=255)
                            gTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                       <  8:38783/146‑1 (MQ=255)
                                gCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTc                                                                                                                 >  5:34137/1‑148 (MQ=255)
                                    aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCAGATCTTcc                                                                                                                         <  4:266562/136‑1 (MQ=255)
                                    aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCAGATCTTcc                                                                                                                         >  3:266562/1‑136 (MQ=255)
                                         gCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGcc                                                                                                       <  5:154800/149‑1 (MQ=255)
                                             cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGt                                                                                                    <  8:229048/148‑1 (MQ=255)
                                             cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGt                                                                                                    <  6:34137/148‑1 (MQ=255)
                                                     cTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACGGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCg                                                                                                                                          <  6:85798/102‑1 (MQ=255)
                                                     cTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACGGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCg                                                                                                                                          >  5:85798/1‑102 (MQ=255)
                                                      ttCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAg                                                                                            >  8:145722/1‑147 (MQ=255)
                                                           tttCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGcc                                                                                      >  8:51788/1‑148 (MQ=255)
                                                                aCGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgtgt                                                                                                                     >  8:187149/1‑112 (MQ=255)
                                                                aCGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgtgt                                                                                                                     <  7:187149/112‑1 (MQ=255)
                                                                 cGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTAcc                                                                               >  5:80565/1‑149 (MQ=255)
                                                                   gCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCt                                                                              >  4:74563/1‑148 (MQ=255)
                                                                    ccTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCAGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGa                                                                            <  6:86011/149‑1 (MQ=255)
                                                                       ggTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg                                                                          >  4:9472/1‑148 (MQ=255)
                                                                        gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGaag                                                                          >  1:231959/1‑145 (MQ=255)
                                                                              ttACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTAc                                                                                                  >  7:163535/1‑117 (MQ=255)
                                                                              ttACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTAc                                                                                                  <  8:163535/117‑1 (MQ=255)
                                                                                 cTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTgcg                                                               >  6:36195/1‑149 (MQ=255)
                                                                                       aTCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGAAGGAGGCGCTGCGCGgcag                                                         <  2:231959/149‑1 (MQ=255)
                                                                                        tCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGgcagc                                                        <  4:196361/149‑1 (MQ=255)
                                                                                           tGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCagcaag                                                     <  6:80565/149‑1 (MQ=255)
                                                                                                gggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGTCGCTGCGCGGCAGCAAGCagaa                                                <  7:51788/149‑1 (MQ=255)
                                                                                                gggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCagaa                                                <  3:9472/149‑1 (MQ=255)
                                                                                                gggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCag                                                  >  6:364133/1‑147 (MQ=255)
                                                                                                        gtcgcTGTAGTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCg                                        >  6:227383/3‑149 (MQ=255)
                                                                                                         gcgcTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGatcatc                                         <  3:74563/147‑1 (MQ=255)
                                                                                                               tactacAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTcc                                  <  5:36195/148‑1 (MQ=255)
                                                                                                                             ccAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCagaaga                                              >  4:186261/1‑122 (MQ=255)
                                                                                                                             ccAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCagaaga                                              <  3:186261/122‑1 (MQ=255)
                                                                                                                                   ccTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg              <  3:105817/148‑1 (MQ=255)
                                                                                                                                   ccTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg              <  7:145722/148‑1 (MQ=255)
                                                                                                                                              tcCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTc  <  5:364133/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTGGCCGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTC  >  NC_002947/4895750‑4896040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑