Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,901,881 G→A 8.0% A449T (GCC→ACC)  PP_4309 → NCS1 family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,901,8810GA 8.0% 143.9 / 2.1 50A449T (GCC→ACC) PP_4309NCS1 family transporter
Reads supporting (aligned to +/- strand):  ref base G (26/20);  new base A (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GGGCGCCCTGCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTACCACGACG  >  NC_002947/4901733‑4902028
                                                                                                                                                     |                                                                                                                                                   
gggCGCCCTGCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGgccg                                                                                                                                                     >  3:48352/1‑149 (MQ=255)
      ccTGCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCGCTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCg                                                                                                                                               >  8:147879/1‑149 (MQ=255)
        tGCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTg                                                                                                                                             >  6:315190/1‑149 (MQ=255)
        tGCTAGGCCCGCTGTACGGCGTGATCATGACCGACTACTGCTTGCTGCACAAAGGCTGCATCAACGTGCCGCAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTg                                                                                                                                             >  8:78343/1‑149 (MQ=255)
         gCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTgc                                                                                                                                            >  3:323975/1‑149 (MQ=255)
          cTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCGCTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTgc                                                                                                                                            <  7:147879/148‑1 (MQ=255)
            aGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACACGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTgccgg                                                                                                                                         >  7:122625/1‑149 (MQ=255)
                        cGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATGAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTgg                                                                                                                             >  1:246797/1‑149 (MQ=255)
                         ggCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGc                                                                                                                            <  8:122625/149‑1 (MQ=255)
                                        gACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCt                                                                                                             >  1:240773/1‑149 (MQ=255)
                                        gACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCt                                                                                                             >  2:224931/1‑149 (MQ=255)
                                                       cgcAAAGGCTGCATCTACGTGCCGGAGCTGTATACCGAGCACCCGGCCTGCGCCTATCACTACAGCAAGGG‑GATCATGTTGCGCGCCGTGGCCGCCTTCGTGCCGGCAGCACTGCTGGGCATCGTTCTGGGCCTGGTGCCCAACTTcc                                                                                               <  8:110907/148‑1 (MQ=255)
                                                       cgcAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTc                                                                                                <  1:224931/147‑1 (MQ=255)
                                                              gCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATc                                                                                       <  4:48352/149‑1 (MQ=255)
                                                                tGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATcgc                                                                                     >  5:287934/1‑149 (MQ=255)
                                                                  cATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATcgc                                                                                     <  2:240773/147‑1 (MQ=255)
                                                                     cAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGt                                                                                                          <  1:110101/123‑1 (MQ=255)
                                                                     cAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGt                                                                                                          >  2:110101/1‑123 (MQ=255)
                                                                      aaCGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGtt                                                                               >  2:192103/1‑149 (MQ=255)
                                                                       aCGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTtc                                                                              <  7:78343/149‑1 (MQ=255)
                                                                         gTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTtctc                                                                            <  8:74520/149‑1 (MQ=255)
                                                                            ccGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAg                                                                                             >  1:186215/1‑129 (MQ=255)
                                                                            ccGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAg                                                                                             <  2:186215/129‑1 (MQ=255)
                                                                             cGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTgg                                                                        <  2:246797/149‑1 (MQ=255)
                                                                              ggAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGc                                                                       <  1:192103/149‑1 (MQ=255)
                                                                                   tGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCACCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGAtt                                                                  >  2:14530/1‑149 (MQ=255)
                                                                                   tGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCACCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGAtt                                                                  >  2:20069/1‑149 (MQ=255)
                                                                                     tataCCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGAtt                                                                  <  3:166485/147‑1 (MQ=255)
                                                                                     tataCCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGAtt                                                                  <  6:287934/147‑1 (MQ=255)
                                                                                          cGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCg                                                           >  7:149837/1‑149 (MQ=255)
                                                                                               aCCCGGCCGGCGCCTATCACTACAGCAAGGGACATCAACCTCCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAgg                                                                                            >  1:212665/1‑112 (MQ=255)
                                                                                               aCCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAgg                                                                                            <  2:212665/111‑1 (MQ=255)
                                                                                                cccGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATcg                                                     >  7:175681/1‑149 (MQ=255)
                                                                                                cacggccggcGCCTATCACTACAGCAAGGG‑AATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGCCCATCGTGCTGGCCCTGCTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGACGGCATcg                                                     >  6:168333/3‑149 (MQ=255)
                                                                                                      cggcGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATcgccgccg                                               >  5:57269/1‑149 (MQ=255)
                                                                                                             tATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTtc                                                                              <  1:55375/111‑1 (MQ=255)
                                                                                                             tATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTtc                                                                              >  2:55375/1‑111 (MQ=255)
                                                                                                               tCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCGGTCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCtata                                        <  5:315190/147‑1 (MQ=255)
                                                                                                               tCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCACCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATAc                                       <  1:14530/148‑1 (MQ=255)
                                                                                                               tCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCACCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATAc                                       <  1:20069/148‑1 (MQ=255)
                                                                                                                     cAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGGCGGCCGCACTGCTGGCCATCGTGCTTGCCCTGGTGCCAAACTTCCAGGGCATGGCGCCGTTCTGCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGGTTa                                <  5:168333/149‑1 (MQ=255)
                                                                                                                      aGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTAt                               >  8:69200/1‑149 (MQ=255)
                                                                                                                        cAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCg                                                           >  4:74370/1‑119 (MQ=255)
                                                                                                                        cAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCg                                                           <  3:74370/119‑1 (MQ=255)
                                                                                                                        cAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCtat                                         >  6:258886/1‑137 (MQ=255)
                                                                                                                        cAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCtat                                         <  5:258886/137‑1 (MQ=255)
                                                                                                                         aaGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATcgc                            >  2:280532/1‑149 (MQ=255)
                                                                                                                             g‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCa                        >  6:265807/1‑149 (MQ=255)
                                                                                                                                    aCCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAAc                  >  6:35435/1‑149 (MQ=255)
                                                                                                                                          gcgcCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCTTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCAt            >  6:288102/1‑149 (MQ=255)
                                                                                                                                            gcCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTa          >  8:278706/1‑149 (MQ=255)
                                                                                                                                                    cgccTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTACCacgacg  >  6:89222/1‑149 (MQ=255)
                                                                                                                                                    cgccTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTACCacgacg  >  5:224894/1‑149 (MQ=255)
                                                                                                                                                    cgccTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTACCacgacg  >  3:113074/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGGCGCCCTGCTAGGCCCGCTGTACGGCGTGATCATGTCCGACTACTGGTTGCTGCGCAAAGGCTGCATCAACGTGCCGGAGCTGTATACCGAGCACCCGGCCGGCGCCTATCACTACAGCAAGGG‑CATCAACCTGCGCGCCGTGGCCGCCTTCGTGCCGGCCGCACTGCTGGCCATCGTGCTGGCCCTGGTGCCCAACTTCCAGGGCATCGCGCCGTTCTCCTGGCTGATTGGTGCCGGCATCGCCGCCGCCCTATACCTGCTTATCGCGCCACGTAACCGCCATTACCACGACG  >  NC_002947/4901733‑4902028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑