Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,902,466 G→T 6.5% L127L (CTG→CTT)  PP_4310 → hydantoin racemase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,902,4660GT 6.5% 200.0 / 3.8 62L127L (CTG→CTT) PP_4310hydantoin racemase
Reads supporting (aligned to +/- strand):  ref base G (26/32);  new base T (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CATGGCCGCGAAGGCCTGCAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCT  >  NC_002947/4902320‑4902614
                                                                                                                                                   |                                                                                                                                                    
cATGGCCGCGAAGGCCTGCAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGaa                                                                                                                                                    <  3:261984/149‑1 (MQ=255)
        cgAAGGCCTGCAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCgg                                                                                                                                            <  8:132656/149‑1 (MQ=255)
               cTGCAGGAACTGCTCAACGTGCCTGTGGTGGACATAACCGACGCCGCCGCCAGCACGGCAATGTACCTATGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTa                                                                                                                                     <  6:91496/149‑1 (MQ=255)
                  cAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAg                                                                                                                                                   >  6:18475/1‑132 (MQ=255)
                  cAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAg                                                                                                                                                   <  5:18475/132‑1 (MQ=255)
                     gAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCg                                                                                                                               <  7:58013/149‑1 (MQ=255)
                                     cTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGc                                                                                                                 >  3:340294/1‑147 (MQ=255)
                                        tggtggACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGc                                                                                                             <  1:19763/148‑1 (MQ=255)
                                          gtggACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAg                                                                                                          >  3:59811/1‑149 (MQ=255)
                                           tggACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGc                                                                                                         >  3:213810/1‑149 (MQ=255)
                                              aCATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCggg                                                                                                      >  3:329971/1‑149 (MQ=255)
                                                  tACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGc                                                                                                             >  8:153683/1‑138 (MQ=255)
                                                  tACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGc                                                                                                             <  7:153683/138‑1 (MQ=255)
                                                   cccGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCCCGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTgc                                                                                                                           <  8:334469/122‑1 (MQ=255)
                                                   aCCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTgc                                                                                                                           >  7:334469/1‑123 (MQ=255)
                                                     cGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGTAAAGCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCg                                                                                                >  1:92184/1‑149 (MQ=255)
                                                       aCGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCa                                                                                                           >  8:102226/1‑135 (MQ=255)
                                                       aCGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCa                                                                                                           <  7:102226/135‑1 (MQ=255)
                                                          ccgccgccAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGctg                                                                                          <  4:213810/149‑1 (MQ=255)
                                                                   gCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGc                                                                                                                              <  1:185896/104‑1 (MQ=255)
                                                                   gCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGc                                                                                                                              >  2:185896/1‑104 (MQ=255)
                                                                   gCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGctggaactggaa                                                                                 <  7:157766/149‑1 (MQ=255)
                                                                   cgtcGGTAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGctggaactggaa                                                                                 <  5:163446/146‑1 (MQ=255)
                                                                       ggCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGCAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGTAAGcc                                                                              >  7:207774/1‑148 (MQ=255)
                                                                       ggCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCg                                                                             >  8:115349/1‑149 (MQ=255)
                                                                              tACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCt                                                                      >  3:242709/1‑149 (MQ=255)
                                                                                  tAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCgg                                                                  >  2:308835/1‑149 (MQ=255)
                                                                                       cACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                >  6:299335/1‑130 (MQ=255)
                                                                                       cACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                <  5:299335/130‑1 (MQ=255)
                                                                                        aCGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTg                                                            >  5:175600/1‑149 (MQ=255)
                                                                                        aCGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTg                                                            <  1:308835/149‑1 (MQ=255)
                                                                                        aCGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTg                                                            <  4:242709/149‑1 (MQ=255)
                                                                                          gCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGa                                                          <  4:85674/149‑1 (MQ=255)
                                                                                           ccTTTTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTgc                                                                                                                           <  3:176518/83‑1 (MQ=255)
                                                                                           ccTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCCCCTGAAGCTCTCGGGCCTGTATGACCGCTgc                                                                                                                           >  4:176518/1‑83 (MQ=255)
                                                                                            cTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTg                                                                                                                  <  4:10911/91‑1 (MQ=255)
                                                                                               ttCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGa                                                     >  6:157107/1‑149 (MQ=255)
                                                                                                 ccGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCg                                                              <  7:59106/138‑1 (MQ=255)
                                                                                                 ccGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCg                                                              >  8:59106/1‑138 (MQ=255)
                                                                                                  cGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGAtt                                                   <  6:175600/148‑1 (MQ=255)
                                                                                                  cGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTg                                                  >  4:127253/1‑149 (MQ=255)
                                                                                                    tagt‑aACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTc                                                <  5:256723/149‑1 (MQ=255)
                                                                                                      gt‑aACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGa                                              <  4:59811/149‑1 (MQ=255)
                                                                                                      gt‑aACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGa                                              <  7:115349/149‑1 (MQ=255)
                                                                                                            cACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAgg                                         >  3:158140/1‑149 (MQ=255)
                                                                                                                 ttGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGTCGTGGAGGCGATTGTCGCGCacggcgcg                                    >  3:27442/1‑142 (MQ=255)
                                                                                                                   gACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCgg                                                                  >  7:192102/1‑117 (MQ=255)
                                                                                                                   gACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCgg                                                                  <  8:192102/117‑1 (MQ=255)
                                                                                                                        cACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCg                             >  2:307589/1‑149 (MQ=255)
                                                                                                                          ccGTGCCATTGATCGAGGACCGCCTTAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                <  8:66254/96‑1 (MQ=255)
                                                                                                                          ccGTGCCATTGATCGAGGACCGCCTTAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                <  8:71081/96‑1 (MQ=255)
                                                                                                                          ccGTGCCATTGATCGAGGACCGCCTTAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                >  7:66254/1‑96 (MQ=255)
                                                                                                                          ccGTGCCATTGATCGAGGACCGCCTTAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAg                                                                                >  7:71081/1‑96 (MQ=255)
                                                                                                                                cATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAg                                          <  4:310880/128‑1 (MQ=255)
                                                                                                                                 aTTGATCGAGGACCGCCTGATGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTg                                                            <  8:113080/109‑1 (MQ=255)
                                                                                                                                 aTTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTg                                                            >  7:113080/1‑109 (MQ=255)
                                                                                                                                        gAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAgg              <  5:157107/148‑1 (MQ=255)
                                                                                                                                        gAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGc             <  3:127253/149‑1 (MQ=255)
                                                                                                                                         aGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGcc                              >  2:213083/1‑131 (MQ=255)
                                                                                                                                         aGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGcc                              <  1:213083/131‑1 (MQ=255)
                                                                                                                                         aGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGcc            <  4:158140/149‑1 (MQ=255)
                                                                                                                                             ccGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAgg        <  8:207774/149‑1 (MQ=255)
                                                                                                                                                   gAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCt  >  3:155177/1‑149 (MQ=255)
                                                                                                                                                   gAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCt  >  3:229602/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATGGCCGCGAAGGCCTGCAGGAACTGCTCAACGTGCCTGTGGTGGACATTACCGACGCCGCCGCCAGCACGGCAATGTACCTAGGCCACGCCTATTCCGTAGT‑AACCACGCTTGACCGCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCT  >  NC_002947/4902320‑4902614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑