Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,930,408 G→C 7.0% L189V (CTG→GTG)  cheZ ← protein phosphatase CheZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,930,4080GC 7.0% 227.9 / 4.5 71L189V (CTG→GTG) cheZprotein phosphatase CheZ
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base C (3/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATT  >  NC_002947/4930265‑4930554
                                                                                                                                               |                                                                                                                                                   
ctgACCTTCACCCCGATTCTGATGTTTTTCTCGATCTTTTTCTGCACGCTATTGATCGTGGTCATGTTTTATACCGTCAAAGCGGTCGACCTGGCTGGCCATCAGCACCTGCTTGAGGAGGTTGCTTTCCAGCTCGGTCACCAGtgtg                                                                                                                                                 <  4:89090/146‑1 (MQ=255)
cGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGtgtg                                                                                                                                                 <  5:215531/148‑1 (MQ=255)
   aCCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGtgtg                                                                                                                                                 <  8:171644/145‑1 (MQ=255)
   aCCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGtgtg                                                                                                                                                 >  7:171644/1‑145 (MQ=255)
   aCCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGa                                                                                                                                             >  2:23296/1‑149 (MQ=255)
     cTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGAcg                                                                                                                                           >  4:79467/1‑149 (MQ=255)
      ttCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCATCTCGGTCACCAGTGTGGTGAcgc                                                                                                                                          >  2:82976/1‑149 (MQ=255)
                gTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCAc                                                                                                                                <  1:23296/149‑1 (MQ=255)
                 tCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACATGGCTGGCCATCAGAACCAGCTTGAGCAGGTTGATTTCAACCTCTGTCACCAGTGTGGTGACGCGCTTGATCAcc                                                                                                                               >  5:181424/1‑149 (MQ=255)
                    gATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTgg                                                                                                                            <  3:34136/149‑1 (MQ=255)
                      tGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATc                                                                                                                                  <  7:12883/141‑1 (MQ=255)
                      tGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATc                                                                                                                                  >  8:12883/1‑141 (MQ=255)
                       gTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCg                                                                                                                         >  7:17095/1‑149 (MQ=255)
                              tcGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGt                                                                                                                  >  6:330263/1‑149 (MQ=255)
                              tcGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGt                                                                                                                  >  2:152752/1‑149 (MQ=255)
                                gATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCa                                                                                                                     <  4:283650/144‑1 (MQ=255)
                                gATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCa                                                                                                                     >  3:283650/1‑144 (MQ=255)
                                gATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTc                                                                                                                 <  4:119476/148‑1 (MQ=255)
                                gATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGAGACGCGCTTGATCACCTGGCCGGTCAGGTcc                                                                                                                >  1:213518/1‑149 (MQ=255)
                                gATCCTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtgg                                                                                                                                                >  4:341910/1‑117 (MQ=255)
                                gATCCTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtgg                                                                                                                                                <  3:341910/117‑1 (MQ=255)
                                 aTCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCt                                                                                                               >  8:54038/1‑149 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                             <  3:79467/146‑1 (MQ=255)
                                      tttCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATa                                                                                                           <  5:330263/148‑1 (MQ=255)
                                           gCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccgagg                                                                                                                                       >  4:7724/1‑110 (MQ=255)
                                           gCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccgagg                                                                                                                                       <  3:7724/115‑6 (MQ=255)
                                               gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtggt                                                                                                                                               <  1:262762/103‑1 (MQ=255)
                                               gCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTgtggt                                                                                                                                               >  2:262762/1‑103 (MQ=255)
                                                 aaTTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAg                                                                                               >  8:1993/1‑149 (MQ=255)
                                                      aTCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaaaa                                                                                          >  4:144944/1‑149 (MQ=255)
                                                          tGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCATCTCGGTCACCAGTGTGGTGACGCGCTAGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTc                                                                                      <  1:82976/149‑1 (MQ=255)
                                                           ggTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccg                                                                                                                                          >  2:289412/1‑94 (MQ=255)
                                                           ggTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCACACTGGTGAccg                                                                                                                                          <  1:289412/96‑3 (MQ=255)
                                                            gTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaaaa                                                                                          >  7:135812/1‑143 (MQ=255)
                                                            gTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCaaaa                                                                                          <  8:135812/143‑1 (MQ=255)
                                                             tCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                                    >  6:143346/1‑132 (MQ=255)
                                                             tCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                                    <  5:143346/132‑1 (MQ=255)
                                                             tCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGtt                                                                                   <  8:17095/149‑1 (MQ=255)
                                                             tCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGtt                                                                                   <  5:97343/149‑1 (MQ=255)
                                                                tGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                             <  8:314071/120‑1 (MQ=255)
                                                                tGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGa                                                                                                             >  7:314071/1‑120 (MQ=255)
                                                                tGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCATGATAGTCCTGGGCCAGCAAAATGTCGTTGAg                                                                                >  8:132232/1‑149 (MQ=255)
                                                                             cAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAAcc                                                                   >  2:61276/1‑149 (MQ=255)
                                                                                        aCCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTAcc                                                       >  2:147284/1‑149 (MQ=255)
                                                                                          ctggctggCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTg                                                                                                     <  2:309079/102‑1 (MQ=255)
                                                                                          ctggctggCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTg                                                                                                     >  1:309079/1‑102 (MQ=255)
                                                                                             gctggcCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCgg                                                  <  1:152752/149‑1 (MQ=255)
                                                                                               tggcCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTgg                                                                                                    <  8:333422/98‑1 (MQ=255)
                                                                                                  ccATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACG‑TTGCGGTTACCCGCGGCGCtg                                             >  8:205117/1‑149 (MQ=255)
                                                                                                   cATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACGTGGCCGGTCAGGTCATGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCtgt                                            <  7:132232/149‑1 (MQ=255)
                                                                                                   cATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCtgt                                            >  2:76185/1‑149 (MQ=255)
                                                                                                        gCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGt                                        >  2:248828/1‑148 (MQ=255)
                                                                                                         cACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGTAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCa                                      <  7:54038/149‑1 (MQ=255)
                                                                                                            cAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGt                                        <  1:334664/144‑1 (MQ=255)
                                                                                                            cAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGAGGTTACCCTCGGCGCTGTGCGt                                        >  2:334664/1‑144 (MQ=255)
                                                                                                             aGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTTGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGaa                                  <  6:181424/149‑1 (MQ=255)
                                                                                                                   aGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTc                            <  7:1993/149‑1 (MQ=255)
                                                                                                                        gTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGt                             <  2:255286/143‑1 (MQ=255)
                                                                                                                        gTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGt                             >  1:255286/1‑143 (MQ=255)
                                                                                                                        gTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTc                       >  5:326238/1‑149 (MQ=255)
                                                                                                                         ttGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCg                      >  3:150321/1‑149 (MQ=255)
                                                                                                                           gCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGc                     <  1:76185/148‑1 (MQ=255)
                                                                                                                            gtttCCACCTGGGTCACCAGTGTGGGGACGGGGTTGCTGACCTGGGCGGTCAGGTCCTGTTTGTGCTGGGCCAGCAAAATGGGGTTGTGGTGGCCGGTAACCATTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGc                     <  1:248828/147‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCtt                   <  1:147284/149‑1 (MQ=255)
                                                                                                                            cTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCtt                   <  3:144944/149‑1 (MQ=255)
                                                                                                                                ccACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGAc                >  6:177117/1‑148 (MQ=255)
                                                                                                                                     tCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCGGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGc                                          <  5:95120/117‑1 (MQ=255)
                                                                                                                                     tCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCAGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGc                                          >  6:95120/1‑117 (MQ=255)
                                                                                                                                      gcgTCACCACACTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCa         >  2:125510/3‑149 (MQ=255)
                                                                                                                                             cAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAAtt  >  3:326455/1‑149 (MQ=255)
                                                                                                                                             cAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAAt   >  4:341186/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CGGACCTTCACCCCGAGTCGGATGTTTTTCTCGATCTTTTTCTGCACGCAATTGATCGTGGTCATGTTTTATACCGGCAAAGCGGTCGACCTGGCTGGCCATCAGCACCAGCTTGAGCAGGTTGCTTTCCACCTCGGTCACCAGTGTGGTGACGCGCTTGATCACCTGGCCGGTCAGGTCCTGATAGTCCTGGGCCAGCAAAATGTCGTTGAGGTGCCCGGAAACC‑TTGCGGTTACCCTCGGCGCTGTGCGTCAGGAAACTGTCGACTCGCTTGACCAGATCACGAAATT  >  NC_002947/4930265‑4930554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑