Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,953,574 C→T 6.5% V286V (GTG→GTA)  PP_4363 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,953,5740CT 6.5% 182.7 / 2.0 61V286V (GTG→GTA) PP_4363two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (27/30);  new base T (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

CTGGTCGTCGGCGGTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTGGCATTCATCTCGC  >  NC_002947/4953430‑4953722
                                                                                                                                                |                                                                                                                                                     
cTGGTCGTCGGCGGTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACt‑t                                                                                                                                                  >  1:6482/1‑149 (MQ=255)
         ggcggTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGTACT‑TGGCCATCCa                                                                                                                                         >  3:22355/1‑149 (MQ=255)
         ggcggTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGTACT‑TGGCCATCCa                                                                                                                                         >  3:17763/1‑149 (MQ=255)
          gcggTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCAc                                                                                                                                        <  4:243164/149‑1 (MQ=255)
                tCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTCCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAg                                                                                                                                  >  2:53311/1‑149 (MQ=255)
                  gATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGc                                                                                                                                >  5:272235/1‑149 (MQ=255)
                   aTCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGc                                                                                                                                >  3:39937/1‑148 (MQ=255)
                    tCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGa                                                                                                                              <  6:154633/149‑1 (MQ=255)
                      acacCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGAt                                                                                                                             <  6:272235/148‑1 (MQ=255)
                      acacCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATa                                                                                                                            <  4:39937/149‑1 (MQ=255)
                            tCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCg                                                                                                                                      <  6:165627/133‑1 (MQ=255)
                            tCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCg                                                                                                                                      >  5:165627/1‑133 (MQ=255)
                              tGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAg                                                                                                                    >  5:164346/1‑149 (MQ=255)
                                aCAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTCCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGc                                                                                                                  <  1:53311/149‑1 (MQ=255)
                                  agcagcAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCa                                                                                                                 >  6:189559/1‑148 (MQ=255)
                                    cagcaACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATAc                                                                                                              >  2:241564/1‑149 (MQ=255)
                                        aaCAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGc                                                                                                           >  1:88097/1‑148 (MQ=255)
                                                   gCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAg                                                                                                                           >  4:108400/1‑121 (MQ=255)
                                                   gCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAg                                                                                                                           <  3:108400/121‑1 (MQ=255)
                                                     ccGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑Tg                                                                                                                                                 >  7:242278/1‑97 (MQ=255)
                                                     ccGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑Tg                                                                                                                                                 <  8:242278/97‑1 (MQ=255)
                                                        gTGCCATCGGCAACACCTCCGTGCTGTCATAGAAACGCTCAGCGGCCAGAATGCCCACCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACTCCGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCAc                                                                                           <  1:270380/149‑1 (MQ=255)
                                                        gTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCAcc                                                                                          <  7:22537/149‑1 (MQ=255)
                                                                   aaCACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATa                                                                                                                            <  4:30824/104‑1 (MQ=255)
                                                                         tCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCa                                                                                                                        <  1:65178/102‑1 (MQ=255)
                                                                         tCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCa                                                                                                                        >  2:65178/1‑102 (MQ=255)
                                                                           cGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTga                                                                       <  6:164346/149‑1 (MQ=255)
                                                                               cTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGAt                                                                    <  2:88097/148‑1 (MQ=255)
                                                                               cTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATCCCACACCTCCACCGAGCCGCGCTGCATGCTGAGAt                                                                    <  5:189559/148‑1 (MQ=255)
                                                                                  tCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGag                                                                >  2:160841/1‑149 (MQ=255)
                                                                                    aTCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGag                                                                >  5:183133/1‑147 (MQ=255)
                                                                                    aTCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGag                                                                >  7:242513/1‑147 (MQ=255)
                                                                                     tCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGagcag                                                             <  3:61339/149‑1 (MQ=255)
                                                                                     tCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGagcag                                                             >  2:117534/1‑149 (MQ=255)
                                                                                     tCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGagcag                                                             <  1:219580/149‑1 (MQ=255)
                                                                                        aaaCGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGagcagcag                                                          >  3:126927/1‑149 (MQ=255)
                                                                                        aaaCGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGagcagcag                                                          >  7:138414/1‑149 (MQ=255)
                                                                                              tCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGca                                                      >  4:284242/1‑147 (MQ=255)
                                                                                                aGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGcacaca                                                    <  8:242513/147‑1 (MQ=255)
                                                                                                aGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGcaca                                                    <  6:183133/147‑1 (MQ=255)
                                                                                                    gCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGTACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGaa                                              <  4:22355/149‑1 (MQ=255)
                                                                                                    gCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGTACT‑TGGCCATCCACAGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGaa                                              <  4:17763/149‑1 (MQ=255)
                                                                                                       aGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCca                                                                                                    >  7:177362/1‑92 (MQ=255)
                                                                                                       aGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCca                                                                                                    <  8:177362/92‑1 (MQ=255)
                                                                                                       aGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACat                                           <  3:284242/149‑1 (MQ=255)
                                                                                                              ccAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCAcc                                    >  2:79151/1‑149 (MQ=255)
                                                                                                               cAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCg                                   >  8:287436/1‑149 (MQ=255)
                                                                                                                  cGGCAGGTGCCGAGAACTCAGTTACGACAGCACT‑TGGCCATCCACAGAAAGGCGATAGCCATCATGAATACCGCCATTCCACACCTCCACCTAGCCGCGCTGCATGCTGAGATTGAGCATCAGCGCACAGCAGAACATATCCAACggaa                                <  8:102303/149‑3 (MQ=255)
                                                                                                                            cgcgAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCtt                      <  1:117534/149‑1 (MQ=255)
                                                                                                                             gcgAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGAATGAGCAGCAGCGCACAGCAGaa                                              >  2:236872/1‑124 (MQ=255)
                                                                                                                             gcgAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGAATGAGCAGCAGCGCACAGCAGaa                                              <  1:236872/124‑1 (MQ=255)
                                                                                                                                aaCTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGc                  <  2:6482/149‑1 (MQ=255)
                                                                                                                                   tCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTg               <  3:235828/149‑1 (MQ=255)
                                                                                                                                   gaaGTGCTGGCGGCACT‑TGTCCATCCAGCGCCAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTTAGATTGAGCAGCAGCGCACAGCAGAACATAGcccc                                     <  2:43563/125‑3 (MQ=255)
                                                                                                                                     aGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGACCATATCCACCGGCAGGATGCGCTTGAGCTTGGc             >  3:65416/1‑149 (MQ=255)
                                                                                                                                     aGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTgg              >  2:225255/1‑148 (MQ=255)
                                                                                                                                     aGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTgg              >  2:154884/1‑148 (MQ=255)
                                                                                                                                     aGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTg               <  8:138414/147‑1 (MQ=255)
                                                                                                                                        gCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTg               >  8:316602/1‑144 (MQ=255)
                                                                                                                                        gCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTg               <  7:316602/144‑1 (MQ=255)
                                                                                                                                         ccGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTGGCAtt          <  4:126927/148‑1 (MQ=255)
                                                                                                                                                cACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTgg              >  2:319220/1‑137 (MQ=255)
                                                                                                                                                cACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTgg              <  1:319220/137‑1 (MQ=255)
                                                                                                                                                cACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTGGCATTCATCTcgc  <  1:154884/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTGGTCGTCGGCGGTATCGATCACACCGTCTGACAGCAGCAACAGGCGCTCGCCCGGTGCCAGCGGCAACACCTCCGTGCTGTCATCGAAACGCTCAGCGGCCAGAATGCCCAGCGGCAGGTGCCGCGAACTCAGTGCCGACAGCACT‑TGGCCATCCACCGACAGGCGATAGCCATCAGGCATACCGCCATTCCACACCTCCACCGAGCCGCGCTGCATGCTGAGATTGAGCAGCAGCGCACAGCAGAACATATCCACCGGCAGGATGCGCTTGAGCTTGGCATTCATCTCGC  >  NC_002947/4953430‑4953722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑