Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,970,868	G→T	6.6%	A233D (GCC→GAC)	PP_4379 ←	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,970,868	0	G	T	6.6%	190.8 / 3.0	60	A233D (GCC→GAC)	PP_4379	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

TGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAGGTGCTCGGCACCG  >  NC_002947/4970722‑4971008                                                                                                                                                   |                                                                                                                                              tgtgCTTCTGCAGCAGCAGCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCATGGTGGATGCAGAAGGCg                                                                                                                                             >  3:302148/1‑149 (MQ=255)     cTTCTGCAGCAGCAGCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑g                                                                                                                                         <  6:172543/148‑1 (MQ=255)     cTTCTGCAGCAGCAGCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑Ga                                                                                                                                        <  4:124603/149‑1 (MQ=255)           cagcagcagCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATcc                                                                                                                                   >  4:318241/1‑148 (MQ=255)             gcagcagcGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCcgac                                                                                                                                <  4:212900/149‑1 (MQ=255)                    cGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCa                                                                                                                         <  1:160144/149‑1 (MQ=255)                      gAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGt                                                                                                                                            >  7:284215/1‑129 (MQ=255)                            tGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCTGAAGTCGTCGGATAT‑CGACGCCTTctgc                                                                                                                       >  5:235986/1‑140 (MQ=255)                            tGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCTGAAGTCGTCGGATAT‑CGACGCCTTctgc                                                                                                                       <  6:235986/143‑4 (MQ=255)                            tGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTc                                                                                                                          >  3:93010/1‑140 (MQ=255)                            tGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTc                                                                                                                          <  4:93010/140‑1 (MQ=255)                              gcggaCTCCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCGTCAAAGCGTCGCGCCATGGCGTCGACAATCGCCGCACTGCCCTGGCGGATGCAGCAGGCGTC‑GATATCAGACGCCTTCAGGCCCGTGGc                                                                                                               >  7:34645/3‑149 (MQ=255)                              gaggaCACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGc                                                                                                               <  6:356964/149‑1 (MQ=255)                                 tacacCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATc                                                                                                            <  3:208652/148‑1 (MQ=255)                                 gaCACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATc                                                                                                            <  3:243976/149‑1 (MQ=255)                                   cacCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATc                                                                                                            >  4:208652/1‑147 (MQ=255)                                   cacCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATc                                                                                                            >  3:9873/1‑147 (MQ=255)                                      cGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCcag                                                                                                         >  5:264973/1‑147 (MQ=255)                                          ttGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTc                                                                                                   <  2:349421/149‑1 (MQ=255)                                               ggTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCa                                                                                                                         >  6:142085/1‑122 (MQ=255)                                               ggTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCa                                                                                                                         <  5:142085/122‑1 (MQ=255)                                                            tCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCAc                                                                                 <  3:318241/149‑1 (MQ=255)                                                               ttGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCtt                                                                              >  1:2583/1‑149 (MQ=255)                                                                  aCGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGAt                                                                           >  3:5496/1‑149 (MQ=255)                                                                  aCGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGAt                                                                           >  3:45189/1‑149 (MQ=255)                                                                    gAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGt                                                                                           <  4:220200/131‑1 (MQ=255)                                                                      aCTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAgg                                                                       <  2:2583/149‑1 (MQ=255)                                                                           tcCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGaa                                                                  <  4:9873/149‑1 (MQ=255)                                                                             cGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGt                                                                <  6:264973/149‑1 (MQ=255)                                                                                   ttGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATa                                                          <  7:67923/149‑1 (MQ=255)                                                                                      ctctTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTNCGCt                                                                                      <  5:154359/118‑1 (MQ=255)                                                                                      ctctTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCt                                                                                      >  6:154359/1‑118 (MQ=255)                                                                                       tctTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTg                                                      >  6:23672/1‑149 (MQ=255)                                                                                         ttCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGaa                                                            >  2:311840/1‑141 (MQ=255)                                                                                         ttCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGaa                                                            <  1:311840/141‑1 (MQ=255)                                                                                          tCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAAt                                                           >  3:86783/1‑141 (MQ=255)                                                                                          tCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAAt                                                           <  4:86783/141‑1 (MQ=255)                                                                                            aaaGCGTCGCGCCACGGCGTCGACAAACGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTggcggc                                                 >  2:205625/1‑149 (MQ=255)                                                                                               gCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGTGAAGTTGAATACCTGGCGGCCGt                                              <  4:45189/149‑1 (MQ=255)                                                                                                      gcCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGGTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCa                                           >  7:185202/1‑145 (MQ=255)                                                                                                      gcCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCa                                           <  8:185202/145‑1 (MQ=255)                                                                                                      gcCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATgaa                                       <  2:277593/149‑1 (MQ=255)                                                                                                      gcCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATg                                         >  1:277593/1‑147 (MQ=255)                                                                                                             gcgcGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTg                                <  3:159960/146‑1 (MQ=255)                                                                                                               tCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGcc                              <  4:5496/149‑1 (MQ=255)                                                                                                                 gACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGt                            >  6:202041/1‑149 (MQ=255)                                                                                                                 gACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGt                            >  2:283425/1‑149 (MQ=255)                                                                                                                        gccgcACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGa                      <  3:157419/148‑1 (MQ=255)                                                                                                                          cgcACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACtt                   >  4:333346/1‑149 (MQ=255)                                                                                                                            cACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATgaagaa                                    >  8:324223/1‑130 (MQ=255)                                                                                                                            cACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATgaagaa                                    <  7:324223/130‑1 (MQ=255)                                                                                                                            cACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACttt                  >  8:277057/1‑148 (MQ=255)                                                                                                                              cTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACAGACTTTCAg               <  5:23672/149‑1 (MQ=255)                                                                                                                                 cccTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAg               <  6:45427/146‑1 (MQ=255)                                                                                                                                 cccTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAg               >  5:45427/1‑146 (MQ=255)                                                                                                                                      gtggATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAGGTGCTc         <  3:3463/147‑1 (MQ=255)                                                                                                                                      gtggATGCAGAAGGCATC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAGGTGCTCgg       >  2:144249/1‑149 (MQ=255)                                                                                                                                           tGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAGGTGCTCGGCACCg  <  3:333346/149‑1 (MQ=255)                                                                                                                                              tgAAGTCGTCGGATAT‑CGACGCCTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCg                             >  6:260599/2‑119 (MQ=255)                                                                                                                                              tgAAGTCGTCGGATAT‑CGACGCCTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCg                             <  5:260599/118‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              TGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACCGTATTGCCGGTTTCCAGCATGTCCTTGACGAACTTCTCCGGGTGTTGCTCTTCAAAGCGTCGCGCCACGGCGTCGACAATCGCCGCACTGCCCTGGTGGATGCAGAAGGCGTC‑GATATCCGACGACTTCAGGCCGCTGGCATCCAGCAGCTCGTGCAGGTGCGCTGGCACCTTGATCAGGGCGAAGTTGAATACCTGGCGGCCGTTCATGAAGAACTTGCCGTCACTGACTTTCAGGTGCTCGGCACCG  >  NC_002947/4970722‑4971008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑