Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,995,524 C→G 9.1% R396R (CGC→CGG) ‡ bkdB → branched‑chain alpha‑keto acid dehydrogenase complex lipoamide acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,995,5240CG 9.1% 134.7 / 4.5 44R396R (CGC→CGG) ‡bkdBbranched‑chain alpha‑keto acid dehydrogenase complex lipoamide acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base G (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATGTA  >  NC_002947/4995381‑4995670
                                                                                                                                               |                                                                                                                                                  
ggTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGt                                                                                                                                               <  7:177489/149‑1 (MQ=255)
 gTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCTTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTc                                                                                                                                              <  6:93165/149‑1 (MQ=255)
    gCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACGCGGTGGTCGaa                                                                                                                                           <  3:227743/149‑2 (MQ=255)
    gCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGa                                                                                                                                            <  2:243593/148‑1 (MQ=255)
         gTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCa                                                                                                                                       <  1:149723/148‑1 (MQ=255)
          tCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGTTGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCAt                                                                                                                                      <  2:202544/148‑1 (MQ=255)
          tCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATg                                                                                                                                     <  3:308283/149‑1 (MQ=255)
             gCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATggtt                                                                                                                                  >  4:225672/1‑147 (MQ=255)
                     gtggtCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCt                                                                                                                          >  5:338855/1‑149 (MQ=255)
                        gtCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGtt                                                                                                                       <  3:225672/149‑1 (MQ=255)
                                           cAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGc                                                                                                    >  6:282439/1‑149 (MQ=255)
                                                 tcgGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTc                                                                                              >  1:135867/1‑149 (MQ=255)
                                                 tcgGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTc                                                                                              <  1:142464/149‑1 (MQ=255)
                                                      gTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGGAAGATGATGATCGTGTGGAGCTCGTTCGACGACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGACGGAGGTGTGCTGGTCGacg                                                                                          >  8:289668/1‑146 (MQ=255)
                                                         aaCCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGaa                                                                                           >  3:3132/1‑144 (MQ=255)
                                                         aaCCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGaa                                                                                           <  4:3132/144‑1 (MQ=255)
                                                           ccGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCg                                                                                    >  8:145074/1‑149 (MQ=255)
                                                            cGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgc                                                                                   >  8:326715/1‑149 (MQ=255)
                                                                  gTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgcctgcct                                                                             >  1:312520/1‑149 (MQ=255)
                                                                        cGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGt                                                                       <  7:326715/149‑1 (MQ=255)
                                                                        cGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGt                                                                       <  7:145074/149‑1 (MQ=255)
                                                                         ggCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTg                                                                      <  5:282439/149‑1 (MQ=255)
                                                                           ccAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTg                                                                      <  6:3586/147‑1 (MQ=255)
                                                                           ccAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTg                                                                      >  5:3586/1‑147 (MQ=255)
                                                                           ccAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGa                                                                    <  6:338855/149‑1 (MQ=255)
                                                                             agtccagATCGACGCGCAGTTGGACGTGGCCAACATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGt                                                                  <  2:135867/143‑1 (MQ=255)
                                                                                        aCGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCaa                                                        >  2:107606/1‑148 (MQ=255)
                                                                                             cAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGaacaac                                                                                       >  2:275710/1‑112 (MQ=255)
                                                                                                    tcgtGCGTAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGa                                           >  7:132793/1‑149 (MQ=255)
                                                                                                    tcgtGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGa                                           >  3:94540/1‑149 (MQ=255)
                                                                                                            aaGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCt                                    <  2:308814/148‑1 (MQ=255)
                                                                                                            aaGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCt                                    >  1:308814/1‑148 (MQ=255)
                                                                                                                  atgaACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGAtt                                                  >  5:243106/1‑128 (MQ=255)
                                                                                                                  atgaACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGAtt                                                  <  6:243106/128‑1 (MQ=255)
                                                                                                                    gaACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATg                                                                                                                                 >  8:297393/1‑47 (MQ=255)
                                                                                                                               gCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGcc                >  3:207292/1‑149 (MQ=255)
                                                                                                                                cTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGccc               >  7:36826/1‑149 (MQ=255)
                                                                                                                                   gTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTgg            >  5:168051/1‑149 (MQ=255)
                                                                                                                                   catCGACCACGCGGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTAcc                                      <  8:60979/121‑1 (MQ=255)
                                                                                                                                   catCGACCACGCGGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTAcc                                      >  7:60979/3‑123 (MQ=255)
                                                                                                                                   catCGACCACGCGGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTgg            >  5:18983/3‑149 (MQ=255)
                                                                                                                                      cGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTAccc                                     <  6:3549/121‑1 (MQ=255)
                                                                                                                                      cGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTAccc                                     >  5:3549/1‑121 (MQ=255)
                                                                                                                                       gACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTggcggc        <  2:39567/149‑1 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCaa                                                        >  7:191173/1‑95 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCaa                                                        <  8:191173/95‑1 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATg    >  1:166828/1‑147 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATg    >  6:177540/1‑147 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATGTa  <  4:207292/149‑1 (MQ=255)
                                                                                                                                             cgcgTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATGTa  <  1:107606/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATGTA  >  NC_002947/4995381‑4995670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑