| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,996,521 | T→G | 6.4% | I304S (ATC→AGC) | lpdV → | branched‑chain alpha‑keto acid dehydrogenase complex dihydrolipoyl dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,996,521 | 0 | T | G | 6.4% | 194.8 / 4.4 | 62 | I304S (ATC→AGC) | lpdV | branched‑chain alpha‑keto acid dehydrogenase complex dihydrolipoyl dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (33/25); new base G (2/2); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
CTTGAGGCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTC > NC_002947/4996376‑4996669 | cTTGAGGCCGACCAGGTACTGGTGGCCGTGAGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCgg > 1:207004/1‑149 (MQ=255) gAGGCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGc < 7:6033/147‑1 (MQ=255) ggCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCg > 7:280158/1‑149 (MQ=255) cAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGc > 8:208874/1‑138 (MQ=255) cAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGc < 7:208874/138‑1 (MQ=255) cAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGACTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCg > 4:261363/1‑136 (MQ=255) cAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGACTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCg < 3:261363/136‑1 (MQ=255) cTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGcccaga < 8:280158/149‑6 (MQ=255) gtggCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGAt > 8:162916/1‑149 (MQ=255) cGTGGGACGCCGGCCACGCACCAAGGGCTTCTACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATg > 5:341490/1‑145 (MQ=255) cGTGGGACGCCGGCCACGCACCAAGGGCTTCTACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATg < 6:341490/145‑1 (MQ=255) cGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTgg > 1:16115/1‑149 (MQ=255) tGGGGCGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGgcg > 6:303677/1‑149 (MQ=255) gACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGCACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCAc < 5:140852/149‑1 (MQ=255) cGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGccca > 6:115142/1‑129 (MQ=255) cGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGccca < 5:115142/132‑4 (MQ=255) gCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCa > 4:163180/1‑149 (MQ=255) gCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGcc > 7:213828/1‑149 (MQ=255) aCCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATAGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGcc > 5:206518/1‑147 (MQ=255) ccAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAg > 2:332491/1‑149 (MQ=255) cAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAgg > 6:355955/1‑149 (MQ=255) cAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAgg > 5:104238/1‑149 (MQ=255) aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg < 7:162916/149‑1 (MQ=255) aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg < 5:303677/149‑1 (MQ=255) aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg < 2:16115/149‑1 (MQ=255) aGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGc < 2:207004/149‑1 (MQ=255) cTTCAACCTGGAATGCCTGCACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCgaga < 2:267910/149‑1 (MQ=255) cTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAatcat < 8:48857/149‑1 (MQ=255) cTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAatca > 7:48857/1‑148 (MQ=255) cTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTc > 6:333706/1‑132 (MQ=255) tGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCgg > 5:340035/1‑148 (MQ=255) gATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAgg > 3:57489/1‑149 (MQ=255) gATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAgg > 1:309914/1‑149 (MQ=255) cGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGAc > 7:67011/1‑149 (MQ=255) cgccgcCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTgg > 6:236571/1‑72 (MQ=255) cgccgcCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTgg < 5:236571/72‑1 (MQ=255) gccgccATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCtt < 8:213828/149‑1 (MQ=255) gCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGa < 5:355955/148‑1 (MQ=255) ccATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACa < 4:57489/149‑1 (MQ=255) cATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGGAAGGCCCGACGCTGTGAACCGACAg > 2:284943/1‑149 (MQ=255) cATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAg > 3:75630/1‑149 (MQ=255) gcTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTgccgcc > 5:316899/1‑149 (MQ=255) cTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGaa < 7:103758/130‑1 (MQ=255) cTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGaa > 8:103758/1‑130 (MQ=255) tGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt > 8:182813/1‑149 (MQ=255) tGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt > 7:247413/1‑149 (MQ=255) tCACACCAGCATGCACAACGTCTGGGCCATGGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt < 6:206518/147‑1 (MQ=255) acCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGa < 3:40761/133‑1 (MQ=255) acCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGa > 4:40761/1‑133 (MQ=255) cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGc < 1:250115/108‑1 (MQ=255) cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGc > 2:250115/1‑108 (MQ=255) cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTa < 1:145173/149‑1 (MQ=255) cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTa < 4:75630/149‑1 (MQ=255) aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGa < 5:171719/119‑1 (MQ=255) aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGa > 6:171719/1‑119 (MQ=255) aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTAc > 8:267515/1‑149 (MQ=255) acaACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg > 2:293859/1‑149 (MQ=255) acaACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg > 1:181816/1‑149 (MQ=255) aCGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTgccgcc < 2:69273/128‑1 (MQ=255) gggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtg < 8:247413/149‑1 (MQ=255) ggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtgg > 7:229247/1‑149 (MQ=255) ggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtg < 1:332491/148‑1 (MQ=255) tCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTc < 2:181816/149‑1 (MQ=255) | CTTGAGGCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTC > NC_002947/4996376‑4996669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |