Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,996,527 A→T 6.7% D306V (GAC→GTC)  lpdV → branched‑chain alpha‑keto acid dehydrogenase complex dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,996,5270AT 6.7% 173.0 / 3.1 60D306V (GAC→GTC) lpdVbranched‑chain alpha‑keto acid dehydrogenase complex dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base T (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01

GGCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTCG  >  NC_002947/4996381‑4996670
                                                                                                                                                  |                                                                                                                                               
ggCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCg                                                                                                                                               >  7:280158/1‑149 (MQ=255)
             cTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGcccaga                                                                                                                                  <  8:280158/149‑6 (MQ=255)
                gtggCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGAt                                                                                                                               >  8:162916/1‑149 (MQ=255)
                     cGTGGGACGCCGGCCACGCACCAAGGGCTTCTACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATg                                                                                                                              >  5:341490/1‑145 (MQ=255)
                     cGTGGGACGCCGGCCACGCACCAAGGGCTTCTACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATg                                                                                                                              <  6:341490/145‑1 (MQ=255)
                     cGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTgg                                                                                                                          >  1:16115/1‑149 (MQ=255)
                       tGGGGCGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGgcg                                                                                                                        >  6:303677/1‑149 (MQ=255)
                          gACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGCACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCAc                                                                                                                     <  5:140852/149‑1 (MQ=255)
                            cGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGccca                                                                                                                                    >  6:115142/1‑129 (MQ=255)
                            cGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCAGCGACGTCGCCGATGGccca                                                                                                                                    <  5:115142/132‑4 (MQ=255)
                                 gCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCa                                                                                                              >  4:163180/1‑149 (MQ=255)
                                      gCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGcc                                                                                                         >  7:213828/1‑149 (MQ=255)
                                        aCCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATAGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGcc                                                                                                         >  5:206518/1‑147 (MQ=255)
                                         ccAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAg                                                                                                      >  2:332491/1‑149 (MQ=255)
                                          cAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAgg                                                                                                     >  6:355955/1‑149 (MQ=255)
                                          cAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAgg                                                                                                     >  5:104238/1‑149 (MQ=255)
                                           aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg                                                                                                    <  7:162916/149‑1 (MQ=255)
                                           aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg                                                                                                    <  5:303677/149‑1 (MQ=255)
                                           aaGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAggg                                                                                                    <  2:16115/149‑1 (MQ=255)
                                            aGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGc                                                                                                   <  2:207004/149‑1 (MQ=255)
                                                cTTCAACCTGGAATGCCTGCACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCgaga                                                                                               <  2:267910/149‑1 (MQ=255)
                                                                cTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAatcat                                                                               <  8:48857/149‑1 (MQ=255)
                                                                cTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAatca                                                                                >  7:48857/1‑148 (MQ=255)
                                                                      cTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTc                                                                                          >  6:333706/1‑132 (MQ=255)
                                                                       tGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCgg                                                                         >  5:340035/1‑148 (MQ=255)
                                                                           gATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAgg                                                                    >  1:309914/1‑149 (MQ=255)
                                                                           gATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAgg                                                                    >  3:57489/1‑149 (MQ=255)
                                                                                 cGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGAc                                                              >  7:67011/1‑149 (MQ=255)
                                                                                    cgccgcCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTgg                                                                                                                                        >  6:236571/1‑72 (MQ=255)
                                                                                    cgccgcCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTgg                                                                                                                                        <  5:236571/72‑1 (MQ=255)
                                                                                     gccgccATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCtt                                                          <  8:213828/149‑1 (MQ=255)
                                                                                              gCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGa                                                  <  5:355955/148‑1 (MQ=255)
                                                                                               ccATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACa                                                <  4:57489/149‑1 (MQ=255)
                                                                                                cATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGGAAGGCCCGACGCTGTGAACCGACAg                                               >  2:284943/1‑149 (MQ=255)
                                                                                                cATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAg                                               >  3:75630/1‑149 (MQ=255)
                                                                                                           gcTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTgccgcc                                    >  5:316899/1‑149 (MQ=255)
                                                                                                            cTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGaa                                                      >  8:103758/1‑130 (MQ=255)
                                                                                                            cTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGaa                                                      <  7:103758/130‑1 (MQ=255)
                                                                                                             tGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt                                  >  8:182813/1‑149 (MQ=255)
                                                                                                             tGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt                                  >  7:247413/1‑149 (MQ=255)
                                                                                                               tCACACCAGCATGCACAACGTCTGGGCCATGGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCgt                                  <  6:206518/147‑1 (MQ=255)
                                                                                                                   acCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGa                                            >  4:40761/1‑133 (MQ=255)
                                                                                                                   acCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGa                                            <  3:40761/133‑1 (MQ=255)
                                                                                                                     cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGc                                                                   >  2:250115/1‑108 (MQ=255)
                                                                                                                     cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGc                                                                   <  1:250115/108‑1 (MQ=255)
                                                                                                                     cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTa                          <  4:75630/149‑1 (MQ=255)
                                                                                                                     cAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTa                          <  1:145173/149‑1 (MQ=255)
                                                                                                                      aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGa                                                       >  6:171719/1‑119 (MQ=255)
                                                                                                                      aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGa                                                       <  5:171719/119‑1 (MQ=255)
                                                                                                                      aGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTAc                         >  8:267515/1‑149 (MQ=255)
                                                                                                                             acaACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg                  >  2:293859/1‑149 (MQ=255)
                                                                                                                             acaACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg                  >  1:181816/1‑149 (MQ=255)
                                                                                                                                aCGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTgccgcc                                    <  2:69273/128‑1 (MQ=255)
                                                                                                                                      gggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtg         <  8:247413/149‑1 (MQ=255)
                                                                                                                                       ggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtgg        >  7:229247/1‑149 (MQ=255)
                                                                                                                                       ggCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAgtggtg         <  1:332491/148‑1 (MQ=255)
                                                                                                                                            tCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTc   <  2:181816/149‑1 (MQ=255)
                                                                                                                                              ggCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTCg  <  6:340035/148‑1 (MQ=255)
                                                                                                                                                 gACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg                  >  1:346856/1‑129 (MQ=255)
                                                                                                                                                 gACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCg                  <  2:346856/129‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGCCGACCAGGTACTGGTGGCCGTGGGACGCCGGCCACGCACCAAGGGCTTCAACCTGGAATGCCTGGACCTGAAGATGAACGGCGCCGCCATTGCCATCGACGAGCGCTGTCACACCAGCATGCACAACGTCTGGGCCATCGGCGACGTCGCTGGCGAACCGATGCTGGCGCACCGGGCCATGGCCCAGGGCGAGATGGTCGCCGAAATCATCGCCGGCAAGGCCCGACGCTTTGAACCGACAGCGATTGCCGCCGTGTGCTTTACCGACCCGGAAGTGGTGGTGGTCG  >  NC_002947/4996381‑4996670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑