Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,059,505 A→T 13.5% N443K (AAT→AAA)  PP_4458 ← opine ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,059,5050AT13.5% 149.4 / 13.3 59N443K (AAT→AAA) PP_4458opine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base T (3/5);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

AGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTG  >  NC_002947/5059357‑5059648
                                                                                                                                                    |                                                                                                                                                
aGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGaaa                                                                                                                                                  >  6:203039/1‑149 (MQ=255)
     tGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑Ga                                                                                                                                            >  5:34384/1‑149 (MQ=255)
      gTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAAt                                                                                                                                                 >  5:321886/1‑144 (MQ=255)
      gTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAAt                                                                                                                                                 <  6:321886/144‑1 (MQ=255)
       tttCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑Ga                                                                                                                                            <  7:279494/147‑1 (MQ=255)
       tttCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACa                                                                                                                                          >  3:87758/1‑149 (MQ=255)
            gACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGct                                                                                                                                     <  5:138912/149‑2 (MQ=255)
            gACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGct                                                                                                                                     <  3:321043/149‑2 (MQ=255)
                  acaaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGctcagtag                                                                                                                               <  2:32114/149‑8 (MQ=255)
                    aaaGGGATGGCGCATATGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCGCAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGAAGCGGGt                                                                                                                               >  5:261563/1‑147 (MQ=255)
                    aaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATAAGCCTCGACCCTGGATTTCCGGGTGGCATCATCAGATACTGAGCAATGTGCGAATTTG‑GACATTGCAGCGGGTgc                                                                                                                             >  2:327681/1‑149 (MQ=255)
                      aGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgcgc                                                                                                                           <  4:87758/149‑1 (MQ=255)
                       gggATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCgg                                                                                                                                 >  1:276962/1‑142 (MQ=255)
                       gggATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCgg                                                                                                                                 <  2:276962/142‑1 (MQ=255)
                         taTGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        <  7:302584/148‑1 (MQ=255)
                           tGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATg                                                                                                                      <  1:28360/149‑1 (MQ=255)
                              cgcATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCa                                                                                                                   <  7:251710/149‑1 (MQ=255)
                                      tcgtcgtGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCg                                                                                                            >  7:54443/1‑148 (MQ=255)
                                            tGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTa                                                                                                     <  8:54443/149‑1 (MQ=255)
                                                         gcgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        <  5:344361/117‑1 (MQ=255)
                                                         gcgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGa                                                                                                                        >  6:344361/1‑117 (MQ=255)
                                                                tttCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCagtag                                                                                  <  6:34384/148‑1 (MQ=255)
                                                                       aaaTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGcc                                                                          >  6:89450/1‑149 (MQ=255)
                                                                             aGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGata                                                                    <  5:203039/149‑1 (MQ=255)
                                                                              gTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               >  6:346287/1‑137 (MQ=255)
                                                                              gTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  5:346287/137‑1 (MQ=255)
                                                                                   gTCTGCGGATCAGACTCGACCCTGGCTTTACGGATGTCATCATCAGCTACTGAGCAATGTCAGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAATCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCTCCCGGATATCGatc                                                              <  1:327681/149‑3 (MQ=255)
                                                                                              aGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               >  6:361083/1‑121 (MQ=255)
                                                                                              aGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  5:361083/121‑1 (MQ=255)
                                                                                               gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  5:70637/1‑149 (MQ=255)
                                                                                               gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  1:320404/1‑149 (MQ=255)
                                                                                                ccTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGACGCCGAATCGTAAATCTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTaaaa                                                  >  3:296095/1‑148 (MQ=255)
                                                                                                ccTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAac                                                 <  5:89450/149‑1 (MQ=255)
                                                                                                 cTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCg                                                                               <  6:166667/118‑1 (MQ=255)
                                                                                                 cTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAaca                                                >  6:70021/1‑149 (MQ=255)
                                                                                                  tCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAacac                                               >  7:281478/1‑149 (MQ=255)
                                                                                                        cTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATcgc                                         <  8:281478/149‑1 (MQ=255)
                                                                                                            cTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGctc                                     >  7:105046/1‑149 (MQ=255)
                                                                                                               tCCGGATGGAATCATCAGATACTTACCAATGTCCTAAATTC‑GACATTGCAGCGGGTGCGCCTATGGCATAAGCCGAATCGTAAAACTCTGTCAGCCATTAGTCGGCGCCCGTATATCGAGCAGCACCGTAAAACACGATCGCGCTCTg                                   <  6:70637/148‑1 (MQ=255)
                                                                                                                ccGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCTGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATc                                                                  <  3:299727/116‑1 (MQ=255)
                                                                                                                ccGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCTGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATc                                                                  >  4:299727/1‑116 (MQ=255)
                                                                                                                     tGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTa                                                     <  7:292692/124‑1 (MQ=255)
                                                                                                                     tGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTa                                                     >  8:292692/1‑124 (MQ=255)
                                                                                                                        catcatCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTc                                <  7:275595/142‑1 (MQ=255)
                                                                                                                        aatcatcAGCTACTGAGCAATGTCAGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGACGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCTAGCAGAACCGTAAAACACGATCGCGCTCTggac                                >  8:275595/2‑140 (MQ=255)
                                                                                                                         atcatcAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGc                        >  7:10052/1‑149 (MQ=255)
                                                                                                                                    cTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              <  8:105046/148‑1 (MQ=255)
                                                                                                                                      gAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTa           >  5:220070/1‑149 (MQ=255)
                                                                                                                                       tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAAt                                                                                                         <  4:128650/53‑1 (MQ=18)
                                                                                                                                       tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAAt                                                                                                         >  3:128650/2‑54 (MQ=18)
                                                                                                                                       tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTg                   <  5:196205/139‑1 (MQ=255)
                                                                                                                                       tgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTg                   >  6:196205/2‑140 (MQ=255)
                                                                                                                                       aGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          <  6:220070/149‑1 (MQ=255)
                                                                                                                                        gCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                               <  6:339342/95‑1 (MQ=255)
                                                                                                                                        gCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                               >  5:339342/1‑95 (MQ=255)
                                                                                                                                          aaTGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          >  6:16545/1‑146 (MQ=255)
                                                                                                                                          aaTGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTaa          <  5:16545/146‑1 (MQ=255)
                                                                                                                                                cGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTg  <  3:169499/148‑1 (MQ=255)
                                                                                                                                                 gAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              <  2:271029/135‑1 (MQ=255)
                                                                                                                                                 gAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg              >  1:271029/1‑135 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGTG  >  NC_002947/5059357‑5059648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑