Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,240 A→C 13.7% intergenic (+231/+61) PP_mr66 → / ← acsA‑I P6/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,2400AC13.7% 69.6 / 3.6 29intergenic (+231/+61)PP_mr66/acsA‑IP6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (15/8);  new base C (2/2);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCC  >  NC_002947/5097094‑5097385
                                                                                                                                                   |                                                                                                                                                 
tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                 >  7:94792/1‑149 (MQ=255)
    tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTg                                                                                                                                              >  5:9654/1‑148 (MQ=255)
      cGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAc                                                                                                                                           <  6:9654/149‑1 (MQ=255)
            aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                   >  6:270753/1‑135 (MQ=255)
            aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑Ta                                                                                                                                                   <  5:270753/135‑1 (MQ=255)
            aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACg                                                                                                                                      >  5:98638/1‑148 (MQ=255)
              cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAg                                                                                                                                                  >  6:56152/1‑134 (MQ=255)
              cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAg                                                                                                                                                  <  5:56152/134‑1 (MQ=255)
              cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGACCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                                   >  1:74101/1‑149 (MQ=255)
                  aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgc                                                                                                                               <  6:98638/149‑1 (MQ=255)
                        cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCaa                                                                                                                         <  1:284611/149‑1 (MQ=255)
                                          ccccTAACCTGTGCGGGGCGGGTGACAGAACATTACCCTTTGATTGTGGGTAAACAATCAAAACGCGGGCTTGCCCGCTATTGGGTGAGGTCAGGGCACAGCT‑TAGCCTTAACTGACGAATTCGGGGGAAAGCCCGCTCCTGCAggggg                                                                                                       <  2:74101/149‑1 (MQ=255)
                                            ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCCGGCAAGCCCGCTCCTGCAGGGGGTa                                                                                                     >  7:257310/1‑149 (MQ=255)
                                                      ggggggGCGGTGACTTAACTGTACGCTTTGATTGTGGGTTAACTATGTAAACGCGGGTTGGCCCGCTAATGCGTTTGGTCTGGGGAAAGGT‑TAGCGTTAACTTAGGCTTTGGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                           <  8:257310/147‑1 (MQ=255)
                                                                           tAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGt                                                                      >  8:97020/1‑149 (MQ=255)
                                                                              ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCCAATGCGTGAGGTCAGGGGACAGCT‑TTGCCTGAACTGACGCATTACCGGGCAAGCCCGCTCCTGGAGGGGGTTTTGCCGGGAAGGGTTTAAGCCGAAACCACGTTCa                                                                   >  2:27729/1‑149 (MQ=255)
                                                                                                             ggCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                    >  3:316999/1‑149 (MQ=18)
                                                                                                                  gCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACAacac                               >  7:10330/1‑149 (MQ=255)
                                                                                                                      gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgtg                                                         >  7:320806/1‑119 (MQ=14)
                                                                                                                      gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTtgtg                                                         <  8:320806/119‑1 (MQ=14)
                                                                                                                              gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCCAACCCGGGTCAGcc                    >  7:221640/1‑148 (MQ=255)
                                                                                                                                  ggTCAGGGCACAGCT‑TCCCCTGAACTGACGAATTGGCGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGATTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg                <  1:27729/148‑1 (MQ=255)
                                                                                                                                     cAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTc              >  2:241175/1‑147 (MQ=255)
                                                                                                                                      aGGGGACAGGT‑TTGCCTGAACTGACGCATTCGCGGGCAATCCCGCTCCTGCAGGGGGTATTTCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag           <  8:10330/149‑1 (MQ=255)
                                                                                                                                       ggggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga          <  7:97020/149‑1 (MQ=255)
                                                                                                                                        gggACAGTT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGACGAAGCCAGGTTCATAGCCATGTGGGAATCGATCAGGTGCTGTACCACAccc                             >  4:20464/1‑129 (MQ=255)
                                                                                                                                          gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGatat       >  1:316558/1‑149 (MQ=255)
                                                                                                                                               cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGccc  <  1:241175/149‑1 (MQ=255)
                                                                                                                                               cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc   >  5:81295/1‑148 (MQ=255)
                                                                                                                                               cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc   >  2:59926/1‑148 (MQ=255)
                                                                                                                                                t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCAGGAAGGGTTTACGCCAAAGCAAGGTTAATCGCATTGTGGGTATCGATCAGGTGCTGTACAAAACCCGTGTCAGCCAAGGACGAAATAACgaca  >  8:218325/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCC  >  NC_002947/5097094‑5097385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑