Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,097,489 A→T 9.0% S635T (TCG→ACG)  acsA‑I ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,097,4890AT 9.0% 126.2 / 2.3 44S635T (TCG→ACG) acsA‑Iacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (24/16);  new base T (2/2);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAGCCTCGG  >  NC_002947/5097342‑5097635
                                                                                                                                                    |                                                                                                                                                    
ggTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGaa                                                                                                                                                     <  5:160693/149‑1 (MQ=255)
             acCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGAGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGCGCCGa                                                                                                                                        >  2:295493/1‑149 (MQ=255)
                                gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCtt                                                                                                                                 >  4:80179/1‑137 (MQ=255)
                                gagaTATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCtt                                                                                                                                 <  3:80179/137‑1 (MQ=255)
                                     atCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCACATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttctt                                                                                                                  >  7:301377/1‑147 (MQ=255)
                                        gCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCt                                                                                                             >  5:279873/1‑149 (MQ=255)
                                            aGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAg                                                                                                         >  8:257171/1‑149 (MQ=255)
                                                    gtACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAAtt                                                                                                <  7:257171/149‑1 (MQ=255)
                                                       cTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGc                                                                                              <  1:295493/148‑1 (MQ=255)
                                                          aCCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGc                                                                                          >  2:188837/1‑149 (MQ=255)
                                                            cGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTg                                                                                        >  4:137304/1‑149 (MQ=255)
                                                              gTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAGCGAGCAATTGcgcctg                                                                                         >  4:232984/1‑142 (MQ=255)
                                                                   gATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTcaa                                                                                 >  6:199246/1‑147 (MQ=255)
                                                                       ttGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttct                                                                                                                   <  1:19205/112‑1 (MQ=255)
                                                                       ttGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGttct                                                                                                                   >  2:19205/1‑112 (MQ=255)
                                                                             aGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCAtt                                                                       >  7:178310/1‑149 (MQ=255)
                                                                                 tGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAgg                                                                   <  6:279873/149‑1 (MQ=255)
                                                                                   cggcgCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCAATGCCGGCATCGAggc                                                                  <  5:199246/148‑2 (MQ=255)
                                                                                                tGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATa                                                    >  8:321238/1‑148 (MQ=255)
                                                                                                 gCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATaaa                                                  >  7:232962/1‑149 (MQ=255)
                                                                                                   ccGAGCGGGTCTTCGGCAGCCCCGGCGCGCCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAt                                                 >  1:20959/1‑149 (MQ=255)
                                                                                                   ccGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCgg                                                                           <  7:300849/123‑1 (MQ=255)
                                                                                                   ccGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGATGGCCTCGATGCCgg                                                                           >  8:300849/1‑123 (MQ=255)
                                                                                                    cGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAtgc                                               >  5:42376/1‑149 (MQ=255)
                                                                                                          ggTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGTAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAtgccctgcc                                         >  6:118390/1‑149 (MQ=255)
                                                                                                          ggTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAtgccctgcc                                         <  3:137304/149‑1 (MQ=255)
                                                                                                                cGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTg                                    <  6:42376/148‑1 (MQ=255)
                                                                                                                 ggCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGTAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGAt                                  <  5:118390/149‑1 (MQ=255)
                                                                                                                 ggCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGAt                                  >  2:47534/1‑149 (MQ=255)
                                                                                                                    aGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCtttagcct                                >  4:169933/1‑143 (MQ=255)
                                                                                                                      ccccGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAt                                                 >  6:153504/1‑129 (MQ=255)
                                                                                                                      ccccGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAAt                                                 <  5:153504/129‑1 (MQ=255)
                                                                                                                      ccccGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCAt                             >  4:12054/1‑149 (MQ=255)
                                                                                                                       cccGGCGC‑CCACTTGATCACATCCGGCGAATCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTACAT‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGTCGACATACACATAAATGCCCTGCCCTTTGATGTCa                              <  1:47534/148‑1 (MQ=255)
                                                                                                                           gcgc‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGa                                   >  8:36381/1‑138 (MQ=255)
                                                                                                                           gcgc‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGa                                   <  7:36381/138‑1 (MQ=255)
                                                                                                                                  aCTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGc                        <  8:69995/143‑1 (MQ=255)
                                                                                                                                  aCTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGc                        >  7:69995/1‑143 (MQ=255)
                                                                                                                                      gATCACATCCGGCGTAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTg                                                                                        >  5:157013/1‑76 (MQ=255)
                                                                                                                                      gATCACATCCGGCGTAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTg                                                                                        <  6:157013/76‑1 (MQ=255)
                                                                                                                                        tCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACt            >  4:91727/1‑149 (MQ=255)
                                                                                                                                            aTCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAg        >  7:148712/1‑149 (MQ=255)
                                                                                                                                                 gCGAAGCAATCCGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAGCCTCg   <  8:232962/149‑1 (MQ=255)
                                                                                                                                                  cGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAGCCTCgg  <  7:321238/149‑1 (MQ=255)
                                                                                                                                                  cGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAGCCTCgg  <  8:301377/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTACTCACCGGTGGCGATCTTGCGCAGGATGCGGCGCATGATCTTGCCCGAGCGGGTCTTCGGCAGCCCCGGCGC‑CCACTGGATCACATCCGGCGAAGCAATCGGGCCGATCTCCTTGCGCACCCAGTTCTTCAGCTCCAG‑GCGCAATTGCTCGCTGGCCTCGATGCCGGCATTGAGGGT‑GACATACACATAAATGCCCTGCCCTTTGATGTCATGCGGCACGCCAACCACTGCAGCCTCGG  >  NC_002947/5097342‑5097635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑