Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,098,621 C→T 7.4% V257V (GTG→GTA)  acsA‑I ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,098,6210CT 7.4% 156.2 / 2.3 54V257V (GTG→GTA) acsA‑Iacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base T (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

TAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCGGGCGA  >  NC_002947/5098475‑5098764
                                                                                                                                                  |                                                                                                                                               
taAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCAc                                                                                                                                               >  5:70833/1‑149 (MQ=255)
taAAGGATGAACAGCGCCTCTTAAGCACCCATTTCTTTGGGGGCGCAGTCGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGACACCGGTGCCCTTGCACACGATGATATTCTGCAc                                                                                                                                               >  2:255306/1‑149 (MQ=255)
  aaGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTTGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACgc                                                                                                                                             <  7:30180/149‑1 (MQ=255)
   aGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACgct                                                                                                                                            >  6:122507/1‑149 (MQ=255)
          aCAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGtt                                                                                                                                     >  7:248612/1‑149 (MQ=255)
             gcgcCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCa                                                                                                                                  <  8:248612/149‑1 (MQ=255)
             gcgcCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCa                                                                                                                                  <  6:70833/149‑1 (MQ=255)
              cgcCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAg                                                                                                                                 <  4:146780/149‑1 (MQ=255)
               gcCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAgg                                                                                                                                <  5:122507/149‑1 (MQ=255)
                      cAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTg                                                                                                                            <  6:64913/146‑1 (MQ=255)
                      cAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGt                                                                                                                          >  1:129278/1‑148 (MQ=255)
                               tttCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAgg                                                                                                                >  5:121543/1‑149 (MQ=255)
                                   tttGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGa                                                                                                            >  8:152672/1‑149 (MQ=255)
                                         ggCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACgctg                                                                                                                                           <  7:307742/112‑1 (MQ=255)
                                         ggCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACgctg                                                                                                                                           >  8:307742/1‑112 (MQ=255)
                                           cgcTGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTTGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGTGTTGGTCAGCGCCAGGTCGACGTTGGcc                                                                                                    <  1:255306/149‑1 (MQ=255)
                                                    tGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGAAGTTGGCCTTGAGCggg                                                                                           >  5:330309/1‑149 (MQ=255)
                                                            aCTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGAc                                                                                                           <  6:278081/125‑1 (MQ=255)
                                                            aCTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGAc                                                                                                           >  5:278081/1‑125 (MQ=255)
                                                            aCTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTAcggcg                                                                                   <  6:121543/149‑1 (MQ=255)
                                                            aCTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTAcggcg                                                                                   >  5:335676/1‑149 (MQ=255)
                                                                  atcaGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGccac                                                                              >  1:187140/1‑148 (MQ=255)
                                                                  atcaGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGccac                                                                              <  2:187140/148‑1 (MQ=255)
                                                                     aGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGt                                                                                         <  1:319742/134‑1 (MQ=255)
                                                                     aGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGt                                                                                         >  2:319742/1‑134 (MQ=255)
                                                                       gTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGTACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACcacgca                                                                        >  5:296048/1‑149 (MQ=255)
                                                                       gTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGTACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACcacgca                                                                        >  3:90286/1‑149 (MQ=255)
                                                                       gTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACcacgca                                                                        >  7:39052/1‑149 (MQ=255)
                                                                       gTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACcacgca                                                                        >  7:105165/1‑149 (MQ=255)
                                                                                ccAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTtcgt                                                               >  5:328209/1‑149 (MQ=255)
                                                                                 cAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTtcgtc                                                              >  4:15910/1‑149 (MQ=255)
                                                                                     atgtgcgGTGCTGGTGCCAGGCAATGTCGGCACCGGTGGGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCa                                                          <  5:93698/145‑1 (MQ=255)
                                                                                          cgGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGTTCGACGTTGGCGTGCAGAGGGGTACGGCGGCCACCACGCACGCCTACGACGGCa                                                          >  6:93698/1‑144 (MQ=255)
                                                                                                   tGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCtt                                            >  8:232001/1‑149 (MQ=255)
                                                                                                   tGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCtt                                            <  7:232001/149‑1 (MQ=255)
                                                                                                      cAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGa                                         <  8:39052/149‑1 (MQ=255)
                                                                                                          cAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTg                                     <  8:105165/149‑1 (MQ=255)
                                                                                                            aTGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGTACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCa                                   <  6:296048/149‑1 (MQ=255)
                                                                                                            aTGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGTACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCa                                   <  4:90286/149‑1 (MQ=255)
                                                                                                               tCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTc                                <  3:15910/149‑1 (MQ=255)
                                                                                                                 gCCACCGGTGCGCTTGCACACGATGATTTTGTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCga                              >  1:29968/1‑149 (MQ=255)
                                                                                                                 gCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCga                              <  2:29968/149‑1 (MQ=255)
                                                                                                                  ccACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCgat                             >  8:275913/1‑149 (MQ=255)
                                                                                                                      cGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATa                         >  7:212511/1‑149 (MQ=255)
                                                                                                                        gTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCgat                             >  3:158589/1‑143 (MQ=255)
                                                                                                                        gTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCgat                             <  4:158589/143‑1 (MQ=255)
                                                                                                                                  acacGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCcagccagc             >  3:288635/1‑149 (MQ=255)
                                                                                                                                  acacGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGCTCGACGTTGGCATTGAGAGGGGTAAGGCGGCAACCACGCACGCCTACGTCGGCAGTGATCACCACCTTGGATTTGAAGTCGATGATACGGCcagccagc             >  7:163605/1‑149 (MQ=255)
                                                                                                                                        tgatTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCg       <  3:84004/149‑1 (MQ=255)
                                                                                                                                           ttttCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGTTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCggg     <  6:335676/148‑1 (MQ=255)
                                                                                                                                           ttttCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCggg     <  8:212511/148‑1 (MQ=255)
                                                                                                                                              tCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCGGGCga  <  7:152672/148‑1 (MQ=255)
                                                                                                                                                tGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCgatga                           >  5:49506/1‑121 (MQ=255)
                                                                                                                                                tGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCgatga                           <  6:49506/121‑1 (MQ=255)
                                                                                                                                                  cACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGccagccag              <  8:270397/132‑1 (MQ=255)
                                                                                                                                                  cACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGccagccag              >  7:270397/1‑132 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TAAAGGATGAACAGCGCCTCTTCAGCACCCATTTCTTTGGGGGCGCAGTGGCTGGAGGCCACTTTCATCAGGTCTTCGTACCAGATGTCGCGGTGCTGGTGCCAGGCAATGTCGCCACCGGTGCGCTTGCACACGATGATTTTCTGCACGCTGCTGGTTTCAGGGTTGGTCAGCGCCAGGTCGACGTTGGCCTTGAGCGGGGTACGGCGGCCACCACGCACGCCTTCGTCGGCAGTGATCACCACCTTGGATTTGCAGTCGATGATACGGCCAGCCAGCGCCTCGGGCGA  >  NC_002947/5098475‑5098764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑