Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,112,952	T→C	7.1%	S165G (AGC→GGC)	PP_4497 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,112,952	0	T	C	7.1%	244.4 / 4.6	84	S165G (AGC→GGC)	PP_4497	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (39/39);  new base C (3/3);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTTCGGCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTGTTCACCGCAACGCCCCGCACGTCC  >  NC_002947/5112807‑5113100                                                                                                                                                   |                                                                                                                                                     tctTTCGGCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCtt                                                                                                                                                   >  5:322411/1‑149 (MQ=255)    ttCGGCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAg                                                                                                                                                >  8:147528/1‑149 (MQ=255)     tCGGCTCACGAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAg                                                                                                                                                <  7:68833/148‑1 (MQ=255)     tCGGCTCACCAACCTCCTCGTCCCCAATGCCGGCAGCCGCCAACGCCTGCAGGGGCGCAGGCACCGGCGCATCATCCGGGTCCAGTTGCGGCGCGGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAg                                                                                                                                                <  6:263763/148‑1 (MQ=255)     tCGGCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAg                                                                                                                                                <  8:32150/148‑1 (MQ=255)        gCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGtt                                                                                                                                             >  2:78742/1‑148 (MQ=255)           cACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGaaa                                                                                                                                         >  4:196149/1‑149 (MQ=255)            aCCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTTCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAAt                                                                                                                                        >  3:231375/1‑149 (MQ=255)            aCCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAAt                                                                                                                                        >  1:294979/1‑149 (MQ=255)               aaCCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGtt                                                                                                                                             >  4:126879/1‑141 (MQ=255)               aaCCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGtt                                                                                                                                             <  3:126879/141‑1 (MQ=255)                aCCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATc                                                                                                                                    <  8:153033/149‑1 (MQ=255)                  ctcctcGTCCCCAAGGCTGGCATCCGCCAACGCCTTCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCga                                                                                                                                  <  4:231375/149‑1 (MQ=255)                  ctcctcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATc                                                                                                                                    >  8:42085/1‑147 (MQ=255)                  ctcctcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCga                                                                                                                                  <  2:294979/149‑1 (MQ=255)                  ctcctcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCga                                                                                                                                  <  7:42085/149‑1 (MQ=255)                    cctcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTg                                                                                                                                                  >  6:352357/1‑131 (MQ=255)                    cctcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTg                                                                                                                                                  <  5:352357/131‑1 (MQ=255)                      tcGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGaaa                                                                                                                              >  8:291366/1‑149 (MQ=255)                        gTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGaaa                                                                                                                              <  8:41362/147‑1 (MQ=255)                           cccAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GAGCCTTGAggac                                                                                                                                             <  5:312463/129‑3 (MQ=255)                           cccAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GAGCCTTGAggac                                                                                                                                             >  6:312463/1‑127 (MQ=255)                            ccAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCtt                                                                                                                        >  4:115418/1‑149 (MQ=255)                            ccAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCtt                                                                                                                        >  1:122163/1‑149 (MQ=255)                            ccAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCtt                                                                                                                        >  8:34778/1‑149 (MQ=255)                                   tGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTtggtggtg                                                                                                                 >  2:86042/1‑149 (MQ=255)                                   tGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCCCTTGAGGTTGAAATGATCGAGAAACGCCTtggtggtg                                                                                                                 >  1:306897/1‑149 (MQ=255)                                   tGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GAGCCTTGAgga                                                                                                                                              >  7:103442/1‑119 (MQ=255)                                   tGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GAGCCTTGAgga                                                                                                                                              <  8:103442/120‑2 (MQ=255)                                     gCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGc                                                                                                               <  7:147528/149‑1 (MQ=255)                                     gCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCCCTTGAGGTTGAAATGATCGAGAAACGCCTtggtggtgg                                                                                                                <  2:306897/148‑1 (MQ=255)                                        tCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGaa                                                                                                            <  7:34778/149‑1 (MQ=255)                                          cGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACa                                                                                                          <  2:122163/149‑1 (MQ=255)                                          cGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACa                                                                                                          >  4:48683/1‑149 (MQ=255)                                           gCCAACGCCTGCATGTGCGCATGCAACGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCATCTCGGCCAAGGCCGGGATGTCCTCAATGCCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACa                                                                                                          <  1:78742/149‑1 (MQ=255)                                            ccAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTtggtgg                                                                                                                   <  6:102638/138‑1 (MQ=255)                                            ccAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTtggtgg                                                                                                                   >  5:102638/1‑138 (MQ=255)                                              aaCGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgc                                                                                                      >  8:60286/1‑149 (MQ=255)                                               aCGCCTGCAGGTGCGCAGGGACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCTAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgcc                                                                                                     <  2:74495/149‑1 (MQ=255)                                                cGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgccg                                                                                                    <  3:196149/149‑1 (MQ=255)                                                   cTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGc                                                                                                                           <  1:33325/123‑1 (MQ=255)                                                   cTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGc                                                                                                                           >  2:33325/1‑123 (MQ=255)                                                      cAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgccggccg                                                                                                <  3:48683/147‑1 (MQ=255)                                                             gcAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCAc                                                                                       >  3:15777/1‑149 (MQ=255)                                                               aGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACtt                                                                                     >  5:328867/1‑149 (MQ=255)                                                               aGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACtt                                                                                     >  1:230584/1‑149 (MQ=255)                                                                 gCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTcg                                                                                   >  6:15825/1‑149 (MQ=255)                                                                   aCCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgccggcc                                                                                                 >  6:90380/1‑133 (MQ=255)                                                                   aCCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgccggcc                                                                                                 <  5:90380/133‑1 (MQ=255)                                                                     cGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGc                                                                                                                           >  4:79879/1‑105 (MQ=255)                                                                     cGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGc                                                                                                                           <  3:79879/105‑1 (MQ=255)                                                                      ggCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTa                                                                              <  1:86042/149‑1 (MQ=255)                                                                       gcgcATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAg                                                                             >  1:168014/1‑149 (MQ=255)                                                                         gcATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGcc                                                                           <  7:60286/149‑1 (MQ=255)                                                                             catcCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTc                                                                                    >  6:124429/1‑136 (MQ=255)                                                                             catcCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTc                                                                                    <  5:124429/136‑1 (MQ=255)                                                                               tcCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCAc                                                                     <  4:15777/149‑1 (MQ=255)                                                                                 cGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATc                                                                                                                                    <  1:251264/84‑1 (MQ=255)                                                                                 cGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATc                                                                                                                                    >  2:251264/1‑84 (MQ=255)                                                                                 cGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCAcgc                                                                   <  7:291366/149‑1 (MQ=255)                                                                                  gggTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCgccgg                                                                                                   >  4:74883/1‑116 (MQ=255)                                                                                   ggTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGa                                                                 <  2:230584/149‑1 (MQ=255)                                                                                    gTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGaa                                                                >  4:104766/1‑149 (MQ=255)                                                                                     tCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGACACTTCGCGGTAGCCGACCACGCGAAt                                                               <  6:322411/149‑1 (MQ=255)                                                                                         gTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCAc                                                           <  3:115418/149‑1 (MQ=255)                                                                                             cggcgCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTcg                                                        <  3:104766/148‑1 (MQ=255)                                                                                              ggcgCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTcgcg                                                      >  3:206040/1‑149 (MQ=255)                                                                                                      ttCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTcgc                                                                                  <  3:290850/113‑1 (MQ=255)                                                                                                      ttCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTcgc                                                                                  >  4:290850/1‑113 (MQ=255)                                                                                                      ttCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCcatca                                              >  4:79279/1‑149 (MQ=255)                                                                                                         cATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTc                                                         >  7:249404/1‑135 (MQ=255)                                                                                                         cATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTc                                                         <  8:249404/135‑1 (MQ=255)                                                                                                           tttCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTc                                         <  2:168014/149‑1 (MQ=255)                                                                                                               gcgcAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTtga                                     <  5:15825/149‑1 (MQ=255)                                                                                                                 gcAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTtgat                                    >  7:281113/1‑148 (MQ=255)                                                                                                                      tCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATg                                            <  2:240823/135‑1 (MQ=255)                                                                                                                      tCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATg                                            >  1:240823/1‑135 (MQ=255)                                                                                                                      tCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATAtt                              >  7:323347/1‑149 (MQ=255)                                                                                                                         gCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCt                           <  4:206040/149‑1 (MQ=255)                                                                                                                          ccAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTg                          >  1:249602/1‑149 (MQ=255)                                                                                                                            aaGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTGtt                        <  8:281113/149‑1 (MQ=255)                                                                                                                                 cGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTtgat                                    >  8:13765/1‑132 (MQ=255)                                                                                                                                 cGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTtgat                                    <  7:13765/132‑1 (MQ=255)                                                                                                                                     aGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTGTTCACCGCAAc               >  6:218255/1‑149 (MQ=255)                                                                                                                                                   tctTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTGTTCACCGCAACGCCCCGCACGTcc  >  1:218966/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TCTTTCGGCTCACCAACCTCCTCGTCCCCAAGGCTGGCATCCGCCAACGCCTGCAGGTGCGCAGGCACCGGCGCATCATCCGGGTCAAGTTGCGGCGCTGGTTCCATTTCGCGCAGCTCGGCCAAGGCCGGCAGGTCCTCAA‑GGCTCTTGAGGTTGAAATGATCGAGAAACGCCTTGGTGGTGGCGAACATCGCCGGCCGCCCGGGCACTTCGCGGTAGCCGACCACGCGAATCCACTCGCGCTCCATCATGGTCTTGATGATATTGCTGTTCACCGCAACGCCCCGCACGTCC  >  NC_002947/5112807‑5113100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑