Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,162,208	G→C	7.0%	A326P (GCT→CCT) ‡	PP_4544 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,162,208	0	G	C	7.0%	182.5 / 3.9	57	A326P (GCT→CCT) ‡	PP_4544	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/25);  new base C (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTA  >  NC_002947/5162062‑5162353                                                                                                                                                   |                                                                                                                                                   aGTCGTTCGGCAACACCACCAACGAGTTCAATCTTGAGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGCTGTCGGCCTATGGCCAGTACGTCAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctggct                                                                                                                                                  <  1:263294/149‑1 (MQ=255)          gCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaa                                                                                                                                          >  4:314281/1‑148 (MQ=255)           cAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaaaa                                                                                                                                        <  7:33951/149‑1 (MQ=255)                 caccaACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCaaaa                                                                                                                                  >  8:237031/1‑149 (MQ=255)                   ccaAAGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATg                                                                                                                                >  4:345769/1‑149 (MQ=255)                    caACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATgg                                                                                                                               >  8:259886/1‑149 (MQ=255)                    caACGAGTTAAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATgg                                                                                                                               >  6:256000/1‑149 (MQ=255)                        gAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gc                                                                                                                          <  3:314281/149‑1 (MQ=255)                          gTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcct                                                                                                                        >  2:332706/1‑149 (MQ=255)                           ttCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcctg                                                                                                                       >  2:337597/1‑149 (MQ=255)                           ttCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTGat                                                                                                                                          <  1:133387/131‑2 (MQ=255)                           ttCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTGat                                                                                                                                          >  2:133387/1‑130 (MQ=255)                             cAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCt                                                                                                                                             <  4:279268/126‑1 (MQ=255)                                tCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTCGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcctggagcc                                                                                                                  >  3:322356/1‑149 (MQ=255)                                    gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcctggagcctgga                                                                                                              <  2:223947/149‑1 (MQ=255)                                    gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcctggagcctg                                                                                                                <  2:29414/147‑1 (MQ=255)                                    gtggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gcctggagcctg                                                                                                                <  7:237031/147‑1 (MQ=255)                                     tggAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACGGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGAc                                                                                                             <  3:335080/149‑1 (MQ=255)                                       gAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gc                                                                                                                          <  3:222869/134‑1 (MQ=255)                                       gAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑gc                                                                                                                          >  4:222869/1‑134 (MQ=255)                                       gAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTa                                                                                                           <  5:256000/149‑1 (MQ=255)                                       gAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTa                                                                                                           >  2:270561/1‑149 (MQ=255)                                          ggCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACaa                                                                                                        <  1:332706/149‑1 (MQ=255)                                             tttGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGCAAACCCAAATGGGT‑GCCTGGAGCCTGGACTACAACTa                                                                                                     >  6:5166/1‑149 (MQ=255)                                                ggCCAGATCGACTTCCCTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTAcc                                                                                                   <  7:259886/148‑1 (MQ=255)                                                        cGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGAtgtg                                                                                           >  8:203840/1‑148 (MQ=255)                                                          aCTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAg                                                                                        >  6:335091/1‑149 (MQ=255)                                                            ttCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCg                                                                                      >  8:14176/1‑149 (MQ=255)                                                                   gCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACAGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAAcgcg                                                                               >  5:191565/1‑149 (MQ=255)                                                                     ttGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAAc                                                                                  <  6:207672/144‑1 (MQ=255)                                                                     ttGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAAc                                                                                  >  5:207672/1‑144 (MQ=255)                                                                     ttGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggt                                                                             >  3:180113/1‑149 (MQ=255)                                                                          cATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtggt                                                                          >  2:28690/1‑147 (MQ=255)                                                                            ttCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGCCAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTCGTCAGc                                                                      >  7:60651/1‑149 (MQ=255)                                                                            ttCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGc                                                                      >  7:56669/1‑149 (MQ=255)                                                                            ttCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGc                                                                      <  3:345769/149‑1 (MQ=255)                                                                            ttCCGTTGGCGGCCTATGGCCAGTACGTGAATAACACCGACTGCACCGACGGCGAAGAGCAGGCCTGGCTGGCTGGCCGGAAAACCAAAATGGGT‑GCCTGGAGCCTGTACTACAACTTCCGCGATGTGCAGCGTAACGCGGTTGTCAGc                                                                      <  5:5166/149‑1 (MQ=255)                                                                               cGTTGTCGGCCTATGGCCAGTTCGTGAATAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTCTCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCt                                                                    <  1:270561/149‑1 (MQ=255)                                                                               cGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTg                                                                   >  1:36369/1‑149 (MQ=255)                                                                                   gTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTc                                                                <  5:335091/148‑1 (MQ=255)                                                                                      ggCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCAcc                                                             >  3:100724/1‑148 (MQ=255)                                                                                            tGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTc                                                        <  7:203840/147‑1 (MQ=255)                                                                                                  gTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCg                                                <  8:33281/149‑1 (MQ=255)                                                                                                      gTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACCTCGGCaa                                            >  8:6162/1‑149 (MQ=255)                                                                                                         aagaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACg                                          <  4:180113/148‑1 (MQ=255)                                                                                                          agaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGc                                        <  2:36369/149‑1 (MQ=255)                                                                                                              cacCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                            <  2:123526/109‑1 (MQ=255)                                                                                                              cacCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                            >  1:123526/1‑109 (MQ=255)                                                                                                              cacCGACAGCACCGACGGCGAAGATCAGGCCAGGCTGGCTGGCCTGAAAAAAAAAATGGGT‑GCATGGAGACTAGAATACAAATAACGCCATGTGCATAGTAAAACAGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTaca                                    >  6:333290/1‑149 (MQ=255)                                                                                                               acCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTacac                                   <  4:100724/149‑1 (MQ=255)                                                                                                                       cACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgc                           >  3:306035/1‑149 (MQ=255)                                                                                                                                    tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAg                >  7:167264/3‑147 (MQ=255)                                                                                                                                    tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAg                <  8:167264/145‑1 (MQ=255)                                                                                                                                        aGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCgg                       >  8:302365/1‑136 (MQ=255)                                                                                                                                        aGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCaa           <  1:28690/148‑1 (MQ=255)                                                                                                                                          gCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGa                                                     >  5:66289/1‑104 (MQ=255)                                                                                                                                          gCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGa                                                     <  6:66289/104‑1 (MQ=255)                                                                                                                                           ccTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTc       <  5:333290/149‑1 (MQ=255)                                                                                                                                            ctggctggctggCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACCTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCg      <  7:6162/149‑1 (MQ=255)                                                                                                                                               gctggctggcCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGtt   <  4:306035/149‑1 (MQ=255)                                                                                                                                                ctggctggcCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTATAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTa  <  6:191565/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGAAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGT‑GCCTGGAGCCTGGACTACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGGTTA  >  NC_002947/5162062‑5162353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑