Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,163,869 T→A 7.4% intergenic (‑19/+105) PP_4545 ← / ← hrpA beta‑ketodecanoyl‑ACP synthase/ATP‑dependent helicase HrpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,163,8690TA 7.4% 157.8 / 2.9 54intergenic (‑19/+105)PP_4545/hrpAbeta‑ketodecanoyl‑ACP synthase/ATP‑dependent helicase HrpA
Reads supporting (aligned to +/- strand):  ref base T (21/29);  new base A (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

AAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCTCAATCGCTTGTCA  >  NC_002947/5163724‑5164016
                                                                                                                                                 |                                                                                                                                                   
aagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGt                                                                                                                                                  <  1:149536/149‑1 (MQ=255)
    caccaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCa                                                                                                                                              <  3:42124/149‑1 (MQ=255)
                 ttGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCa                                                                                                                                         >  7:184679/1‑141 (MQ=255)
                 ttGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCa                                                                                                                                         <  8:184679/141‑1 (MQ=255)
                           tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTATTGGCACTAAAGTGCCa                                                                                                                                              >  6:159268/1‑126 (MQ=255)
                           tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTATTGGCACTAAAGTGCCa                                                                                                                                              <  5:159268/126‑1 (MQ=255)
                           tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTAAAGTGCCatt                                                                                                                                            >  6:142626/1‑126 (MQ=255)
                           tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTAAAGTGCCatt                                                                                                                                            <  5:142626/128‑3 (MQ=255)
                                   ggggTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcggc                                                                                                               <  6:253594/149‑1 (MQ=255)
                                   ggggTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcggc                                                                                                               <  8:188964/149‑1 (MQ=255)
                                     ggTGTACAGGGCGGTGCCGCTTATCAGGACGTTATGCACGGTCGTTCCTCTGGTCATAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGcggcgg                                                                                                             <  8:279188/149‑1 (MQ=255)
                                      gtgtACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCggg                                                                                                            <  8:299194/149‑1 (MQ=255)
                                           cAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTAt                                                                                                       >  4:300570/1‑149 (MQ=255)
                                              gCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCg                                                                                                    >  8:25654/1‑149 (MQ=255)
                                              gCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCg                                                                                                    >  7:35084/1‑149 (MQ=255)
                                               ccGGTGCCGCTGAGCACGACGTTATGCACGGTCGTTCCTCTGGTCACAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGc                                                                                                   <  1:116684/149‑1 (MQ=255)
                                                      cGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGc                                                                                            >  1:230329/1‑149 (MQ=255)
                                                      cGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGc                                                                                            <  2:230329/149‑1 (MQ=255)
                                                       gCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGcc                                                                                           <  7:25654/149‑1 (MQ=255)
                                                              acgacgTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGc                                                                                    <  7:250161/149‑1 (MQ=255)
                                                               cgacgTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATc                                                                                                      >  6:283928/1‑130 (MQ=255)
                                                               cgacgTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATc                                                                                                      <  5:283928/130‑1 (MQ=255)
                                                                    ttATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCTTTGCGGccct                                                                              <  3:300570/149‑2 (MQ=255)
                                                                      aTGCACGGTCGTTCCTCTGGTAAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCa                                                                             >  4:59673/1‑148 (MQ=255)
                                                                       tGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCTTTGCGGcccttgg                                                                           <  6:67355/149‑5 (MQ=255)
                                                                          aCGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGc                                                                        >  1:160549/1‑149 (MQ=255)
                                                                                gTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCaaa                                                                  <  2:160549/149‑1 (MQ=255)
                                                                                gTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCa                                                                    >  6:34249/1‑147 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGc                                                                                 >  7:287501/1‑122 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGc                                                                                 <  8:287501/122‑1 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCaaa                                                                  <  7:212617/137‑1 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCaaa                                                                  <  7:207277/137‑1 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCaaa                                                                  >  8:212617/1‑137 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCaaa                                                                  >  8:207277/1‑137 (MQ=255)
                                                                                            aaaGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACaaa                                                      >  4:40370/1‑149 (MQ=255)
                                                                                                gCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAaataat                                                  >  2:152817/1‑149 (MQ=255)
                                                                                                 ccATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGc                                                            <  6:113803/138‑1 (MQ=255)
                                                                                                 ccATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGc                                                            >  5:113803/1‑138 (MQ=255)
                                                                                                   aTCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATg                                                 >  2:319422/1‑147 (MQ=255)
                                                                                                    tCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAg                                              <  3:59673/149‑1 (MQ=255)
                                                                                                       ccACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCGTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGcc                                                           <  4:319076/133‑1 (MQ=255)
                                                                                                       ccACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCGTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGcc                                                           >  3:319076/1‑133 (MQ=255)
                                                                                                        cacaGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGa                                               <  1:82652/144‑1 (MQ=255)
                                                                                                        cacaGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGa                                               >  2:82652/1‑144 (MQ=255)
                                                                                                        cacaGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGt                                            >  5:223336/1‑147 (MQ=255)
                                                                                                        cacaGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGt                                            >  7:61223/1‑147 (MQ=255)
                                                                                                          caGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGc                                        <  8:35084/149‑1 (MQ=255)
                                                                                                                        gAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCAc                                 >  7:328026/1‑142 (MQ=255)
                                                                                                                        gAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCAc                                 <  8:328026/142‑1 (MQ=255)
                                                                                                                        gAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACt                           <  8:61223/148‑1 (MQ=255)
                                                                                                                        gAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACt                           <  6:223336/148‑1 (MQ=255)
                                                                                                                           aCATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCAt                       <  3:40370/149‑1 (MQ=255)
                                                                                                                                  aGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGc                <  5:34249/149‑1 (MQ=255)
                                                                                                                                   ggTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCt               <  4:211119/149‑1 (MQ=255)
                                                                                                                                             aatttAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGCAGGGAATATCCGCAAAGGGCCGCAAAGCGGCACCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATAGTTTCATCAATCGATTg     >  5:81792/3‑149 (MQ=255)
                                                                                                                                                ttAGTGCCAATTCAGGGGCATACGTTTAGCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCTCAATCGCTTGt    >  1:194223/1‑147 (MQ=255)
                                                                                                                                                 tAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCTCAATCGCTTGTc   <  8:143476/147‑1 (MQ=255)
                                                                                                                                                 tAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCTCAATCGCTTGTCa  <  1:319422/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAAGCGGCCCCAAACGCCCCAAAGTTTGCCACAAATAATGGAGGTTAGCCTTCCACCTGACTCCATTGTTTGCTCAATCGCTTGTCA  >  NC_002947/5163724‑5164016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑